Restriction map for
pFN21AE4079
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseRI BsgI BsiWI BsmAI BsmBI
BsmI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstAPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoRI EcoRV EcoT14I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MluI
MluNI MscI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 414 TCCGGA T^CCGGA
AcsI 854 AAATTC [GA]^AATT[CT]
AflIII 376 ACACGT A^C[GA][CT]GT
AgeI 550 ACCGGT A^CCGGT
ApoI 854 AAATTC [GA]^AATT[CT]
BanII 721 GAGCTC G[GA]GC[CT]^C
BciVI 417 GGATAC GTATCC(6/5)
BclI 705 TGATCA T^GATCA
BfaI 142 CTAG C^TAG
BseAI 414 TCCGGA T^CCGGA
BsiEI 1008 CGGCCG CG[GA][CT]^CG
BsiYI 829 CCACCAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 829 CCACCAATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 146 GTCCC GGGAC(10/14)
Bsp1407I 1035 TGTACA T^GTACA
BspEI 414 TCCGGA T^CCGGA
BsrGI 1035 TGTACA T^GTACA
BstBI 1109 TTCGAA TT^CGAA
BstEII 967 GGTTACC G^GT[ACGT]ACC
BstPI 967 GGTTACC G^GT[ACGT]ACC
BstXI 734 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 12 CCGTGG C^C[GA][CT]GG
EaeI 1008 CGGCCG [CT]^GGCC[GA]
EagI 1008 CGGCCG C^GGCCG
EclXI 1008 CGGCCG C^GGCCG
Eco52I 1008 CGGCCG C^GGCCG
EcoO65I 967 GGTTACC G^GT[ACGT]ACC
EcoT22I 1177 ATGCAT ATGCA^T
FbaI 705 TGATCA T^GATCA
HgaI 357 GACGC GACGC(5/10)
MaeI 142 CTAG C^TAG
MaeII 378 ACGT A^CGT
MfeI 561 CAATTG C^AATTG
MroI 414 TCCGGA T^CCGGA
MspA1I 51 CAGCTG C[AC]G^C[GT]G
MunI 561 CAATTG C^AATTG
MwoI 723 GCTCATCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1177 ATGCAT ATGCA^T
NspV 1109 TTCGAA TT^CGAA
PinAI 550 ACCGGT A^CCGGT
PleI 1083 GAGTC GAGTC(4/5)
PpuMI 144 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 51 CAGCTG CAG^CTG
SacI 721 GAGCTC GAGCT^C
SapI 718 GAAGAGC GCTCTTC(1/4)
SfuI 1109 TTCGAA TT^CGAA
SspBI 1035 TGTACA T^GTACA
SspI 845 AATATT AAT^ATT
StuI 1000 AGGCCT AGG^CCT
XcmI 50 CCAGCTGCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 39 GCGG CCGC(-3/-1)
1007 GCGG
AfaI 456 GTAC GT^AC
1036 GTAC
AseI 619 ATTAAT AT^TAAT
1173 ATTAAT
AsnI 619 ATTAAT AT^TAAT
1173 ATTAAT
AspHI 689 GTGCTC G[AT]GC[AT]^C
721 GAGCTC
BbvI 53 GCTGC GCAGC(8/12)
1138 GCTGC
BglII 588 AGATCT A^GATCT
651 AGATCT
BmyI 689 GTGCTC G[AGT]GC[ACT]^C
721 GAGCTC
BpmI 287 CTCCAG CTGGAG(16/14)
1142 CTGGAG
BsaJI 12 CCGTGG C^C[ACGT][ACGT]GG
148 CCCAGG
BsaWI 414 TCCGGA [AT]^CCGG[AT]
550 ACCGGT
BsiHKAI 689 GTGCTC G[AT]GC[AT]^C
721 GAGCTC
Bsp1286I 689 GTGCTC G[AGT]GC[ACT]^C
721 GAGCTC
BsrI 809 ACTGG ACTGG(1/-1)
1101 CCAGT
BstYI 588 AGATCT [GA]^GATC[CT]
651 AGATCT
Cac8I 49 GCCAGC GC[ACGT]^[ACGT]GC
186 GCCAGC
DraII 144 AGGTCCC [GA]G^G[ACGT]CC[CT]
935 GGGGCCT
EcoO109I 144 AGGTCCC [GA]G^G[ACGT]CC[CT]
935 GGGGCCT
HphI 9 TCACC GGTGA(8/7)
1182 TCACC
MflI 588 AGATCT [GA]^GATC[CT]
651 AGATCT
PshBI 619 ATTAAT AT^TAAT
1173 ATTAAT
RsaI 456 GTAC GT^AC
1036 GTAC
SfcI 77 CTGTAG C^T[GA][CT]AG
876 CTACAG
TaqI 716 TCGA T^CGA
1110 TCGA
TseI 53 GCTGC G^C[AT]GC
1138 GCTGC
TthHB8I 716 TCGA T^CGA
1110 TCGA
XhoII 588 AGATCT [GA]^GATC[CT]
651 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AvaII BbsI BpuAI BsrFI
BssKI BstF5I BstNI Cfr10I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I Tsp45I
Tsp509I TspRI