Restriction map for
pFN21AE6533
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MluI MroI
MseI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 162 CCGC CCGC(-3/-1)
AlwNI 50 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 62 CCCGGG C^[CT]CG[GA]G
BalI 176 TGGCCA TGG^CCA
BpmI 82 CTGGAG CTGGAG(16/14)
BsgI 165 CTGCAC GTGCAG(16/14)
BsiYI 33 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 33 CCTCTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 263 GAGAC GTCTC(1/5)
BsmFI 233 GTCCC GGGAC(10/14)
BsmI 138 GCATTC GAATGC(1/-1)
BsoBI 62 CCCGGG C^[CT]CG[GA]G
BsrI 114 CCAGT ACTGG(1/-1)
BstAPI 125 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 170 CATCC GGATG(2/0)
BstXI 312 CCATGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 8 AGATCC [GA]^GATC[CT]
CfoI 148 GCGC GCG^C
DsaI 173 CCATGG C^C[GA][CT]GG
EaeI 176 TGGCCA [CT]^GGCC[GA]
EcoT14I 173 CCATGG C^C[AT][AT]GG
FauI 161 CCCGC CCCGC(4/6)
FokI 170 CATCC GGATG(9/13)
HapII 63 CCGG C^CGG
HhaI 148 GCGC GCG^C
HinP1I 148 GCGC G^CGC
HpaII 63 CCGG C^CGG
MboII 6 GAAGA GAAGA(8/7)
MfeI 126 CAATTG C^AATTG
MflI 8 AGATCC [GA]^GATC[CT]
MluNI 176 TGGCCA TGG^CCA
MscI 176 TGGCCA TGG^CCA
MspA1I 162 CCGCTG C[AC]G^C[GT]G
MspI 63 CCGG C^CGG
MunI 126 CAATTG C^AATTG
NcoI 173 CCATGG C^CATGG
SacI 94 GAGCTC GAGCT^C
SfaNI 305 GCATC GCATC(5/9)
SmaI 62 CCCGGG CCC^GGG
StyI 173 CCATGG C^C[AT][AT]GG
Tsp509I 127 AATT ^AATT
TspRI 115 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 8 AGATCC [GA]^GATC[CT]
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 51 AGCT AG^CT
95 AGCT
AspHI 94 GAGCTC G[AT]GC[AT]^C
195 GTGCTC
BanII 94 GAGCTC G[GA]GC[CT]^C
279 GGGCTC
BcnI 62 CCCGG CC^[GC]GG
63 CCGGG
BsaBI 9 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
69 GATCATTATC
BsiHKAI 94 GAGCTC G[AT]GC[AT]^C
195 GTGCTC
HphI 91 GGTGA GGTGA(8/7)
287 TCACC
MamI 9 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
69 GATCATTATC
MslI 139 CATTCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
168 CACATCCATG
NciI 62 CCCGG CC^[GC]GG
63 CCGGG
NlaIV 58 GGCCCC GG[ACGT]^[ACGT]CC
280 GGCTCC
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AlwI BbvI BmyI BsaJI Bsp1286I
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII HgaI ItaI
MboI MnlI MvaI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI TseI