Restriction map for
pFN21AE6558
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 27 CCGC CCGC(-3/-1)
AfaI 408 GTAC GT^AC
AluI 324 AGCT AG^CT
Alw44I 349 GTGCAC G^TGCAC
AlwNI 440 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 349 GTGCAC G^TGCAC
AspHI 349 GTGCAC G[AT]GC[AT]^C
AvaI 44 CCCGAG C^[CT]CG[GA]G
AvaII 318 GGTCC G^G[AT]CC
BanII 126 GGGCTC G[GA]GC[CT]^C
BbsI 274 GAAGAC GAAGAC(2/6)
BcgI 369 CGAGGTAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 124 CCGGG CC^[GC]GG
BfaI 208 CTAG C^TAG
BpuAI 274 GAAGAC GAAGAC(2/6)
BsaI 132 GGTCTC GGTCTC(1/5)
BsiHKAI 349 GTGCAC G[AT]GC[AT]^C
BsiYI 411 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 411 CCTGCAGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 133 GTCTC GTCTC(1/5)
BsmFI 319 GTCCC GGGAC(10/14)
BsoBI 44 CCCGAG C^[CT]CG[GA]G
BspMI 410 ACCTGC ACCTGC(4/8)
BsrDI 380 CATTGC GCAATG(2/0)
BstAPI 414 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 185 CCAGGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 233 CTCAG C^T[ACGT]AG
EaeI 365 TGGCCG [CT]^GGCC[GA]
FauI 26 CCCGC CCCGC(4/6)
HgaI 218 GCGTC GACGC(5/10)
HincII 220 GTCAAC GT[CT]^[GA]AC
HindII 220 GTCAAC GT[CT]^[GA]AC
MaeI 208 CTAG C^TAG
MaeII 119 ACGT A^CGT
MseI 299 TTAA T^TAA
NciI 124 CCGGG CC^[GC]GG
NcoI 59 CCATGG C^CATGG
NlaIII 60 CATG CATG^
NlaIV 318 GGTCCC GG[ACGT]^[ACGT]CC
RsaI 408 GTAC GT^AC
TaqI 269 TCGA T^CGA
TfiI 103 GATTC G^A[AT]TC
Tru9I 299 TTAA T^TAA
Tsp45I 430 GTCAC ^GT[GC]AC
Tsp509I 375 AATT ^AATT
TthHB8I 269 TCGA T^CGA
XcmI 185 CCAGGGCCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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BmyI 126 GGGCTC G[AGT]GC[ACT]^C
349 GTGCAC
Bsp1286I 126 GGGCTC G[AGT]GC[ACT]^C
349 GTGCAC
BsrFI 3 GCCGGC [GA]^CCGG[CT]
93 GCCGGC
BstF5I 228 CATCC GGATG(2/0)
242 CATCC
BstNI 185 CCAGG CC^[AT]GG
426 CCAGG
Cfr10I 3 GCCGGC [GA]^CCGG[CT]
93 GCCGGC
Cfr13I 188 GGGCC G^G[ACGT]CC
318 GGTCC
DpnII 180 GATC ^GATC
267 GATC
DsaI 59 CCATGG C^C[GA][CT]GG
191 CCGTGG
EcoRII 185 CCAGG ^CC[AT]GG
426 CCAGG
FokI 228 CATCC GGATG(9/13)
242 CATCC
HaeII 6 GGCGCT [GA]GCGC^[CT]
203 AGCGCC
MaeIII 148 GTTAC ^GT[ACGT]AC
430 GTCAC
MboI 180 GATC ^GATC
267 GATC
MboII 264 GAAGA GAAGA(8/7)
274 GAAGA
MvaI 185 CCAGG CC^[AT]GG
426 CCAGG
MwoI 3 GCCGGCGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
414 GCAGTGGGTGC
NaeI 3 GCCGGC GCC^GGC
93 GCCGGC
NdeII 180 GATC ^GATC
267 GATC
NgoMIV 3 GCCGGC G^CCGGC
93 GCCGGC
PstI 137 CTGCAG CTGCA^G
412 CTGCAG
Sau3AI 180 GATC ^GATC
267 GATC
Sau96I 188 GGGCC G^G[ACGT]CC
318 GGTCC
SfcI 137 CTGCAG C^T[GA][CT]AG
412 CTGCAG
TspRI 279 CAGTG CA[GC]TG[ACGT][ACGT]^
415 CAGTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
BbvI BsaJI BssKI Cac8I CfoI
EcoT14I Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MnlI
MspI PleI ScrFI StyI TseI