Restriction map for
pFN21AE2284
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflIII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
BbrPI BbsI BbvCI BclI BglII
BpuAI BsaAI BsaBI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflII 2050 CTTAAG C^TTAAG
Aor51HI 610 AGCGCT AGC^GCT
Asp700I 769 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1822 CCTAGG C^CTAGG
BalI 1726 TGGCCA TGG^CCA
BamHI 1004 GGATCC G^GATCC
BbeI 1674 GGCGCC GGCGC^C
BcgI 690 CGATCCCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2050 CTTAAG C^TTAAG
BlnI 1822 CCTAGG C^CTAGG
BlpI 1847 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1847 GCTCAGC GC^T[ACGT]AGC
BsaI 824 GAGACC GGTCTC(1/5)
BsaWI 632 TCCGGT [AT]^CCGG[AT]
BsrBI 1845 CCGCTC CCGCTC(-3/-3)
CelII 1847 GCTCAGC GC^T[ACGT]AGC
Eco47III 610 AGCGCT AGC^GCT
EcoRV 1459 GATATC GAT^ATC
HgaI 2225 GCGTC GACGC(5/10)
HincII 489 GTCAAC GT[CT]^[GA]AC
HindII 489 GTCAAC GT[CT]^[GA]AC
KasI 1674 GGCGCC G^GCGCC
KspI 7 CCGCGG CCGC^GG
MaeII 258 ACGT A^CGT
MluNI 1726 TGGCCA TGG^CCA
MscI 1726 TGGCCA TGG^CCA
MslI 2001 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1025 GCCGGC GCC^GGC
NarI 1674 GGCGCC GG^CGCC
NcoI 2159 CCATGG C^CATGG
NgoMIV 1025 GCCGGC G^CCGGC
PaeR7I 1832 CTCGAG C^TCGAG
PflMI 1288 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 7 CCGCGG CCGC^GG
ScaI 1657 AGTACT AGT^ACT
SfiI 97 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1674 GGCGCC GGC^GCC
TfiI 433 GAATC G^A[AT]TC
Van91I 1288 CCATACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1832 CTCGAG C^TCGAG
XmnI 769 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 8 CGCG CG^CG
1009 CGCG
AcyI 1674 GGCGCC G[GA]^CG[CT]C
2224 GGCGTC
AocI 511 CCTGAGG CC^T[ACGT]AGG
2246 CCTCAGG
ApaI 96 GGGCCC GGGCC^C
983 GGGCCC
BsaHI 1674 GGCGCC G[GA]^CG[CT]C
2224 GGCGTC
BssSI 33 CACGAG CACGAG(-5/-1)
1201 CTCGTG
BstEII 951 GGTGACC G^GT[ACGT]ACC
1837 GGTCACC
BstPI 951 GGTGACC G^GT[ACGT]ACC
1837 GGTCACC
BstUI 8 CGCG CG^CG
1009 CGCG
BstYI 1004 GGATCC [GA]^GATC[CT]
1921 GGATCT
Bsu36I 511 CCTGAGG CC^T[ACGT]AGG
2246 CCTCAGG
CfoI 611 GCGC GCG^C
1675 GCGC
Eco81I 511 CCTGAGG CC^T[ACGT]AGG
2246 CCTCAGG
EcoO65I 951 GGTGACC G^GT[ACGT]ACC
1837 GGTCACC
HaeII 610 AGCGCT [GA]GCGC^[CT]
1674 GGCGCC
HhaI 611 GCGC GCG^C
1675 GCGC
Hin1I 1674 GGCGCC G[GA]^CG[CT]C
2224 GGCGTC
HinP1I 611 GCGC G^CGC
1675 GCGC
MflI 1004 GGATCC [GA]^GATC[CT]
1921 GGATCT
MseI 881 TTAA T^TAA
2051 TTAA
MvnI 8 CGCG CG^CG
1009 CGCG
TaqI 222 TCGA T^CGA
1833 TCGA
Tru9I 881 TTAA T^TAA
2051 TTAA
TthHB8I 222 TCGA T^CGA
1833 TCGA
XcmI 707 CCACGGATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1337 CCAAGGATGAGTTGG
XhoII 1004 GGATCC [GA]^GATC[CT]
1921 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BanI BanII BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TseI Tsp45I Tsp509I TspRI XmaCI
XmaI