Restriction map for
pFN21AE2148
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflIII 195 ACGTGT A^C[GA][CT]GT
AluI 317 AGCT AG^CT
ApaI 353 GGGCCC GGGCC^C
AvaII 291 GGTCC G^G[AT]CC
BanII 353 GGGCCC G[GA]GC[CT]^C
BbsI 325 GAAGAC GAAGAC(2/6)
BbvI 366 GCTGC GCAGC(8/12)
BclI 200 TGATCA T^GATCA
BglI 419 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 353 GGGCCC G[AGT]GC[ACT]^C
BpmI 331 CTGGAG CTGGAG(16/14)
BpuAI 325 GAAGAC GAAGAC(2/6)
BsmAI 3 GTCTC GTCTC(1/5)
Bsp1286I 353 GGGCCC G[AGT]GC[ACT]^C
BspDI 58 ATCGAT AT^CGAT
BstAPI 30 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 299 CCAATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 233 AGATCC [GA]^GATC[CT]
Cac8I 398 GCCAGC GC[ACGT]^[ACGT]GC
ClaI 58 ATCGAT AT^CGAT
DdeI 179 CTGAG C^T[ACGT]AG
FauI 166 GCGGG CCCGC(4/6)
FbaI 200 TGATCA T^GATCA
HgaI 369 GCGTC GACGC(5/10)
MaeII 195 ACGT A^CGT
MaeIII 371 GTCAC ^GT[ACGT]AC
MflI 233 AGATCC [GA]^GATC[CT]
MseI 98 TTAA T^TAA
MslI 204 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 124 CATG CATG^
PpuMI 290 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 42 AGTACT AGT^ACT
SfiI 418 GGCCGCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 59 TCGA T^CGA
Tru9I 98 TTAA T^TAA
TseI 366 GCTGC G^C[AT]GC
Tsp45I 371 GTCAC ^GT[GC]AC
TthHB8I 59 TCGA T^CGA
XcmI 95 CCATTAAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 233 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 43 GTAC GT^AC
228 GTAC
AlwI 12 GATCC GGATC(4/5)
234 GATCC
BfaI 7 CTAG C^TAG
295 CTAG
BsiYI 356 CCCATAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
429 CCGCACCGTGG
BslI 356 CCCATAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
429 CCGCACCGTGG
BssKI 288 CCAGG ^CC[ACGT]GG
330 CCTGG
BstNI 288 CCAGG CC^[AT]GG
330 CCTGG
DraII 290 AGGTCCT [GA]G^G[ACGT]CC[CT]
380 GGGGCCT
DsaI 423 CCGTGG C^C[GA][CT]GG
434 CCGTGG
EaeI 417 TGGCCG [CT]^GGCC[GA]
426 TGGCCG
EcoO109I 290 AGGTCCT [GA]G^G[ACGT]CC[CT]
380 GGGGCCT
EcoRII 288 CCAGG ^CC[AT]GG
330 CCTGG
HinfI 216 GAATC G^A[ACGT]TC
348 GATTC
HphI 155 TCACC GGTGA(8/7)
203 TCACC
MaeI 7 CTAG C^TAG
295 CTAG
MvaI 288 CCAGG CC^[AT]GG
330 CCTGG
NlaIV 353 GGGCCC GG[ACGT]^[ACGT]CC
380 GGGGCC
RsaI 43 GTAC GT^AC
228 GTAC
ScrFI 288 CCAGG CC^[ACGT]GG
330 CCTGG
TfiI 216 GAATC G^A[AT]TC
348 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI BsaJI BsrI BstF5I Cfr13I
DpnII Fnu4HI FokI HaeIII ItaI
MboI MboII MnlI MwoI NdeII
Sau3AI Sau96I TspRI