Restriction map for
pFN21AE6663
##################################
# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MroI MseI
MunI NaeI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tru9I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 771 GGTACC G^GTACC
AfaI 772 GTAC GT^AC
AflIII 461 ACACGT A^C[GA][CT]GT
AlwNI 647 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 193 CCTGAGG CC^T[ACGT]AGG
Asp718I 771 GGTACC G^GTACC
AvaI 498 CTCGGG C^[CT]CG[GA]G
BalI 113 TGGCCA TGG^CCA
BbeI 805 GGCGCC GGCGC^C
BfaI 181 CTAG C^TAG
BglI 574 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 520 GGTCTC GGTCTC(1/5)
BsgI 703 CTGCAC GTGCAG(16/14)
BsoBI 498 CTCGGG C^[CT]CG[GA]G
BspMI 512 ACCTGC ACCTGC(4/8)
BsrFI 394 ACCGGC [GA]^CCGG[CT]
BstAPI 132 GCACGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 372 GGATG GGATG(2/0)
Bsu36I 193 CCTGAGG CC^T[ACGT]AGG
Cfr10I 394 ACCGGC [GA]^CCGG[CT]
DraIII 133 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 472 GACTTCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 708 CCGTGG C^C[GA][CT]GG
EarI 388 CTCTTC CTCTTC(1/4)
Eco81I 193 CCTGAGG CC^T[ACGT]AGG
EcoT14I 243 CCAAGG C^C[AT][AT]GG
FokI 372 GGATG GGATG(9/13)
HaeII 805 GGCGCC [GA]GCGC^[CT]
KasI 805 GGCGCC G^GCGCC
KpnI 771 GGTACC GGTAC^C
Ksp632I 388 CTCTTC CTCTTC(1/4)
MaeI 181 CTAG C^TAG
MaeIII 763 GTCAC ^GT[ACGT]AC
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
NarI 805 GGCGCC GG^CGCC
NdeI 617 CATATG CA^TATG
PstI 745 CTGCAG CTGCA^G
RsaI 772 GTAC GT^AC
SfoI 805 GGCGCC GGC^GCC
StuI 414 AGGCCT AGG^CCT
StyI 243 CCAAGG C^C[AT][AT]GG
TaqI 299 TCGA T^CGA
Tsp45I 763 GTCAC ^GT[GC]AC
Tsp509I 339 AATT ^AATT
TthHB8I 299 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 130 GAGCAC G[AT]GC[AT]^C
832 GTGCTC
BanI 771 GGTACC G^G[CT][GA]CC
805 GGCGCC
BlpI 813 GCTCAGC GC^T[ACGT]AGC
834 GCTCAGC
BpmI 273 CTGGAG CTGGAG(16/14)
793 CTGGAG
Bpu1102I 813 GCTCAGC GC^T[ACGT]AGC
834 GCTCAGC
BseRI 196 GAGGAG GAGGAG(10/8)
440 GAGGAG
BsiEI 233 CGGCCG CG[GA][CT]^CG
396 CGGCCG
BsiHKAI 130 GAGCAC G[AT]GC[AT]^C
832 GTGCTC
BsmBI 480 CGTCTC CGTCTC(1/5)
353 GAGACG
BsrBI 101 CCGCTC CCGCTC(-3/-3)
281 CCGCTC
BsrDI 203 CATTGC GCAATG(2/0)
592 GCAATG
BstYI 108 GGATCT [GA]^GATC[CT]
720 GGATCT
CelII 813 GCTCAGC GC^T[ACGT]AGC
834 GCTCAGC
CfoI 516 GCGC GCG^C
806 GCGC
DraII 501 GGGTCCC [GA]G^G[ACGT]CC[CT]
537 GGGACCT
EagI 233 CGGCCG C^GGCCG
396 CGGCCG
EclXI 233 CGGCCG C^GGCCG
396 CGGCCG
Eco52I 233 CGGCCG C^GGCCG
396 CGGCCG
EcoO109I 501 GGGTCCC [GA]G^G[ACGT]CC[CT]
537 GGGACCT
HhaI 516 GCGC GCG^C
806 GCGC
HinP1I 516 GCGC G^CGC
806 GCGC
MaeII 383 ACGT A^CGT
463 ACGT
MboII 389 TCTTC GAAGA(8/7)
666 TCTTC
MflI 108 GGATCT [GA]^GATC[CT]
720 GGATCT
NlaIII 37 CATG CATG^
531 CATG
PpuMI 501 GGGTCCC [GA]G^G[AT]CC[CT]
537 GGGACCT
PvuII 175 CAGCTG CAG^CTG
647 CAGCTG
SfcI 172 CTACAG C^T[GA][CT]AG
745 CTGCAG
XhoII 108 GGATCT [GA]^GATC[CT]
720 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanII BbvI BcnI BmyI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI MboI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIV PleI Sau3AI
Sau96I ScrFI TseI TspRI