Restriction map for
pFN21AE6178
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# NO CUT ENZYMES ( 179 enzymes) #
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Acc65I AccI AccII AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbrPI BbsI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
HphI KpnI Ksp632I KspI MaeI
MaeIII MamI MboI MboII MfeI
MflI MluI MroI MseI MslI
MunI MvnI NaeI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tru9I Tsp45I TspRI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 313 GACGTC GACGT^C
AcsI 160 GAATTC [GA]^AATT[CT]
AlwNI 28 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 160 GAATTC [GA]^AATT[CT]
AvaII 327 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 233 GGCGCC G^G[CT][GA]CC
BbeI 233 GGCGCC GGCGC^C
BbvCI 211 GCTGAGG CCTCAGC(-5/-2)
BciVI 20 GTATCC GTATCC(6/5)
BcnI 89 CCCGG CC^[GC]GG
BsaWI 341 ACCGGA [AT]^CCGG[AT]
BseRI 112 GAGGAG GAGGAG(10/8)
BsgI 241 GTGCAG GTGCAG(16/14)
BsmFI 12 GTCCC GGGAC(10/14)
BsrI 5 CCAGT ACTGG(1/-1)
BstXI 291 CCAGCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 234 GCGC GCG^C
DraII 319 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 185 CCATGG C^C[GA][CT]GG
EcoO109I 319 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 160 GAATTC G^AATTC
FauI 48 GCGGG CCCGC(4/6)
HaeII 233 GGCGCC [GA]GCGC^[CT]
HhaI 234 GCGC GCG^C
HinP1I 234 GCGC G^CGC
KasI 233 GGCGCC G^GCGCC
MaeII 314 ACGT A^CGT
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspA1I 53 CAGCTG C[AC]G^C[GT]G
NarI 233 GGCGCC GG^CGCC
NciI 89 CCCGG CC^[GC]GG
NcoI 185 CCATGG C^CATGG
NspI 248 GCATGC [GA]CATG^[CT]
PvuII 53 CAGCTG CAG^CTG
SacI 255 GAGCTC GAGCT^C
SfaNI 70 GATGC GCATC(5/9)
SfoI 233 GGCGCC GGC^GCC
SphI 248 GCATGC GCATG^C
Tsp509I 161 AATT ^AATT
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcyI 233 GGCGCC G[GA]^CG[CT]C
313 GACGTC
AspHI 154 GAGCAC G[AT]GC[AT]^C
255 GAGCTC
BanII 189 GGGCTC G[GA]GC[CT]^C
255 GAGCTC
BsaHI 233 GGCGCC G[GA]^CG[CT]C
313 GACGTC
BsiHKAI 154 GAGCAC G[AT]GC[AT]^C
255 GAGCTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
182 CGGCCA
EcoT14I 15 CCAAGG C^C[AT][AT]GG
185 CCATGG
HapII 90 CCGG C^CGG
342 CCGG
Hin1I 233 GGCGCC G[GA]^CG[CT]C
313 GACGTC
HpaII 90 CCGG C^CGG
342 CCGG
MspI 90 CCGG C^CGG
342 CCGG
StyI 15 CCAAGG C^C[AT][AT]GG
185 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI Bsp1286I BssKI BstAPI
BstNI Cac8I Cfr13I DdeI EcoRII
Fnu4HI HaeIII ItaI MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI