Restriction map for
pFN21AE4134
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# NO CUT ENZYMES ( 106 enzymes) #
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AccIII AclI AflII AflIII AgeI
Alw44I AlwI Aor51HI ApaI ApaLI
AscI AseI AsnI AspI AviII
AvrII BamHI BbrPI BbvCI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstYI BstZ17I
ClaI CpoI DpnII DraI DraIII
DrdI Eco47III EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KspI MamI MboI MfeI
MflI MluI MroI MslI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StyI SwaI Tth111I XbaI
XhoII
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 124 GACGTC GACGT^C
Acc65I 971 GGTACC G^GTACC
AccI 1102 GTCTAC GT^[AC][GT]AC
AccII 528 CGCG CG^CG
AhdI 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 589 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 798 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 971 GGTACC G^GTACC
AspEI 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1559 GGCGCC GGCGC^C
BbsI 100 GTCTTC GAAGAC(2/6)
BciVI 789 GTATCC GTATCC(6/5)
BfaI 1325 CTAG C^TAG
BglI 697 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1432 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1432 GCTGAGC GC^T[ACGT]AGC
BpuAI 100 GTCTTC GAAGAC(2/6)
BsaI 1349 GAGACC GGTCTC(1/5)
BsaWI 847 ACCGGA [AT]^CCGG[AT]
BsiEI 401 CGGCCG CG[GA][CT]^CG
Bsp1407I 1019 TGTACA T^GTACA
BsrDI 822 CATTGC GCAATG(2/0)
BsrFI 1201 GCCGGC [GA]^CCGG[CT]
BsrGI 1019 TGTACA T^GTACA
BssSI 768 CACGAG CACGAG(-5/-1)
BstUI 528 CGCG CG^CG
BstXI 215 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1432 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1201 GCCGGC [GA]^CCGG[CT]
DsaI 793 CCACGG C^C[GA][CT]GG
EagI 401 CGGCCG C^GGCCG
Eam1105I 952 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 401 CGGCCG C^GGCCG
Eco52I 401 CGGCCG C^GGCCG
EcoNI 27 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 517 GATATC GAT^ATC
KasI 1559 GGCGCC G^GCGCC
KpnI 971 GGTACC GGTAC^C
MaeI 1325 CTAG C^TAG
MvnI 528 CGCG CG^CG
NaeI 1201 GCCGGC GCC^GGC
NarI 1559 GGCGCC GG^CGCC
NgoMIV 1201 GCCGGC G^CCGGC
PaeR7I 827 CTCGAG C^TCGAG
PflMI 1366 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 71 GGGACCC [GA]G^G[AT]CC[CT]
SfoI 1559 GGCGCC GGC^GCC
SspBI 1019 TGTACA T^GTACA
StuI 885 AGGCCT AGG^CCT
TaqI 828 TCGA T^CGA
TfiI 1249 GAATC G^A[AT]TC
TthHB8I 828 TCGA T^CGA
Van91I 1366 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 821 CCATTGCTCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 827 CTCGAG C^TCGAG
XmnI 798 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanII 646 GAGCTC G[GA]GC[CT]^C
754 GAGCTC
BsrBI 235 GAGCGG CCGCTC(-3/-3)
601 GAGCGG
BstEII 39 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
BstPI 39 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
DraII 71 GGGACCC [GA]G^G[ACGT]CC[CT]
391 AGGCCCC
EcoO109I 71 GGGACCC [GA]G^G[ACGT]CC[CT]
391 AGGCCCC
EcoO65I 39 GGTGACC G^GT[ACGT]ACC
273 GGTGACC
EcoRI 853 GAATTC G^AATTC
895 GAATTC
FauI 69 GCGGG CCCGC(4/6)
603 GCGGG
HaeII 1383 GGCGCT [GA]GCGC^[CT]
1559 GGCGCC
HgaI 683 GCGTC GACGC(5/10)
941 GCGTC
MseI 179 TTAA T^TAA
836 TTAA
PstI 749 CTGCAG CTGCA^G
976 CTGCAG
SacI 646 GAGCTC GAGCT^C
754 GAGCTC
SfcI 749 CTGCAG C^T[GA][CT]AG
976 CTGCAG
SmaI 278 CCCGGG CCC^GGG
380 CCCGGG
SmlI 827 CTCGAG C^T[CT][GA]AG
1582 CTCAAG
Tru9I 179 TTAA T^TAA
836 TTAA
XmaCI 278 CCCGGG C^CCGGG
380 CCCGGG
XmaI 278 CCCGGG C^CCGGG
380 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AocI ApoI AspHI AvaI AvaII
BalI BanI BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI EaeI EarI Eco81I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MluNI MnlI MscI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PvuII RsaI Sau96I
ScrFI SfaNI TseI Tsp45I Tsp509I
TspRI