Restriction map for
pFN21AE9418
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssSI BstBI BstUI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoT22I FauI FbaI FseI FspI
HapII HincII HindII HpaI HpaII
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 328 GTATAC GT^[AC][GT]AC
AcsI 14 AAATTT [GA]^AATT[CT]
AcyI 145 GGCGTC G[GA]^CG[CT]C
AfaI 540 GTAC GT^AC
AflIII 648 ACATGT A^C[GA][CT]GT
AlwNI 211 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 827 GGGCCC GGGCC^C
ApoI 14 AAATTT [GA]^AATT[CT]
AspHI 1111 GAGCAC G[AT]GC[AT]^C
BanI 846 GGCACC G^G[CT][GA]CC
BbsI 286 GTCTTC GAAGAC(2/6)
BglI 235 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 800 AGATCT A^GATCT
BpuAI 286 GTCTTC GAAGAC(2/6)
BsaHI 145 GGCGTC G[GA]^CG[CT]C
BsaI 252 GGTCTC GGTCTC(1/5)
BsiHKAI 1111 GAGCAC G[AT]GC[AT]^C
BsmBI 163 GAGACG CGTCTC(1/5)
BsmFI 204 GGGAC GGGAC(10/14)
BspLU11I 648 ACATGT A^CATGT
BsrBI 508 GAGCGG CCGCTC(-3/-3)
BsrDI 231 CATTGC GCAATG(2/0)
BsrI 641 ACTGG ACTGG(1/-1)
Bst1107I 328 GTATAC GTA^TAC
BstAPI 210 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 100 GGTGACC G^GT[ACGT]ACC
BstPI 100 GGTGACC G^GT[ACGT]ACC
BstXI 30 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 800 AGATCT [GA]^GATC[CT]
BstZ17I 328 GTATAC GTA^TAC
CfoI 923 GCGC GCG^C
EcoO65I 100 GGTGACC G^GT[ACGT]ACC
EcoRV 66 GATATC GAT^ATC
EcoT14I 239 CCAAGG C^C[AT][AT]GG
HaeII 922 AGCGCC [GA]GCGC^[CT]
HgaI 146 GCGTC GACGC(5/10)
HhaI 923 GCGC GCG^C
Hin1I 145 GGCGTC G[GA]^CG[CT]C
HinP1I 923 GCGC G^CGC
HindIII 676 AAGCTT A^AGCTT
MaeII 356 ACGT A^CGT
MflI 800 AGATCT [GA]^GATC[CT]
MseI 892 TTAA T^TAA
MspA1I 1046 CAGCTG C[AC]G^C[GT]G
NdeI 181 CATATG CA^TATG
PvuII 1046 CAGCTG CAG^CTG
RsaI 540 GTAC GT^AC
SapI 687 GCTCTTC GCTCTTC(1/4)
StyI 239 CCAAGG C^C[AT][AT]GG
Tru9I 892 TTAA T^TAA
XcmI 239 CCAAGGCTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 800 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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BalI 446 TGGCCA TGG^CCA
494 TGGCCA
BanII 685 GGGCTC G[GA]GC[CT]^C
827 GGGCCC
BsgI 579 CTGCAC GTGCAG(16/14)
796 GTGCAG
BstF5I 333 CATCC GGATG(2/0)
40 GGATG
DpnII 360 GATC ^GATC
801 GATC
EaeI 446 TGGCCA [CT]^GGCC[GA]
494 TGGCCA
EarI 526 CTCTTC CTCTTC(1/4)
688 CTCTTC
FokI 333 CATCC GGATG(9/13)
40 GGATG
Ksp632I 526 CTCTTC CTCTTC(1/4)
688 CTCTTC
MboI 360 GATC ^GATC
801 GATC
MluNI 446 TGGCCA TGG^CCA
494 TGGCCA
MscI 446 TGGCCA TGG^CCA
494 TGGCCA
NdeII 360 GATC ^GATC
801 GATC
Sau3AI 360 GATC ^GATC
801 GATC
SexAI 125 ACCTGGT A^CC[AT]GGT
341 ACCTGGT
Tsp509I 15 AATT ^AATT
894 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII HinfI
HphI ItaI MaeIII MboII MnlI
MslI MvaI MwoI NlaIII NlaIV
NspI PleI Sau96I ScrFI SfaNI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I