Restriction map for
pFN21AB5846
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KspI MaeII MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 883 GGTACC G^GTACC
AccII 190 CGCG CG^CG
AciI 204 CCGC CCGC(-3/-1)
AflII 127 CTTAAG C^TTAAG
AseI 1449 ATTAAT AT^TAAT
AsnI 1449 ATTAAT AT^TAAT
Asp718I 883 GGTACC G^GTACC
BanI 883 GGTACC G^G[CT][GA]CC
BciVI 640 GGATAC GTATCC(6/5)
BcnI 210 CCGGG CC^[GC]GG
BfrI 127 CTTAAG C^TTAAG
BglII 1155 AGATCT A^GATCT
BsaBI 641 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 499 GAGACC GGTCTC(1/5)
BsmFI 542 GGGAC GGGAC(10/14)
BstUI 190 CGCG CG^CG
BstXI 420 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 90 CCATGG C^C[GA][CT]GG
EarI 1385 GAAGAG CTCTTC(1/4)
EcoNI 126 CCTTAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 210 CCGG C^CGG
HincII 1415 GTCAAC GT[CT]^[GA]AC
HindII 1415 GTCAAC GT[CT]^[GA]AC
HpaII 210 CCGG C^CGG
KpnI 883 GGTACC GGTAC^C
Ksp632I 1385 GAAGAG CTCTTC(1/4)
MamI 641 GATACATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 210 CCGG C^CGG
MvnI 190 CGCG CG^CG
NciI 210 CCGGG CC^[GC]GG
NcoI 90 CCATGG C^CATGG
NlaIV 883 GGTACC GG[ACGT]^[ACGT]CC
NruI 189 TCGCGA TCG^CGA
NspI 999 ACATGC [GA]CATG^[CT]
PflMI 340 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 737 GACTCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1449 ATTAAT AT^TAAT
SapI 1385 GAAGAGC GCTCTTC(1/4)
ScaI 402 AGTACT AGT^ACT
SspI 438 AATATT AAT^ATT
StuI 317 AGGCCT AGG^CCT
TaqI 1332 TCGA T^CGA
TthHB8I 1332 TCGA T^CGA
Van91I 340 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AlwI 243 GATCC GGATC(4/5)
572 GATCC
AvaII 184 GGACC G^G[AT]CC
466 GGACC
BseRI 352 GAGGAG GAGGAG(10/8)
828 GAGGAG
BsmI 1369 GAATGC GAATGC(1/-1)
1222 GCATTC
HaeIII 318 GGCC GG^CC
507 GGCC
HindIII 1052 AAGCTT A^AGCTT
1137 AAGCTT
MslI 421 CATGAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
958 CACCAATATG
SmlI 127 CTTAAG C^T[CT][GA]AG
668 CTCAAG
XcmI 171 CCAGGATTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
575 CCATGCCAAAGCTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
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AcsI AfaI AluI AlwNI ApoI
Asp700I AspHI BanII BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRI EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII XmnI