Restriction map for
pFN21AE5443
##################################
# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII HphI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 249 AAATTT [GA]^AATT[CT]
AlwI 207 GGATC GGATC(4/5)
ApoI 249 AAATTT [GA]^AATT[CT]
Asp700I 331 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 610 GAGCTC G[AT]GC[AT]^C
AvaII 804 GGACC G^G[AT]CC
BanII 610 GAGCTC G[GA]GC[CT]^C
BciVI 680 GGATAC GTATCC(6/5)
BglI 316 GCCTTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 610 GAGCTC G[AGT]GC[ACT]^C
BsgI 545 CTGCAC GTGCAG(16/14)
BsiHKAI 610 GAGCTC G[AT]GC[AT]^C
BsmBI 765 CGTCTC CGTCTC(1/5)
Bsp1286I 610 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 226 TGTACA T^GTACA
BspMI 776 ACCTGC ACCTGC(4/8)
BsrDI 840 CATTGC GCAATG(2/0)
BsrGI 226 TGTACA T^GTACA
BstYI 207 GGATCT [GA]^GATC[CT]
Cfr13I 804 GGACC G^G[ACGT]CC
DraI 901 TTTAAA TTT^AAA
DraIII 500 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 344 TGGCCG [CT]^GGCC[GA]
EcoNI 356 CCTTTGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 698 GTCGAC GT[CT]^[GA]AC
HindII 698 GTCGAC GT[CT]^[GA]AC
MflI 207 GGATCT [GA]^GATC[CT]
NdeI 650 CATATG CA^TATG
NlaIV 724 GGTTCC GG[ACGT]^[ACGT]CC
SacI 610 GAGCTC GAGCT^C
SalI 698 GTCGAC G^TCGAC
SapI 612 GCTCTTC GCTCTTC(1/4)
Sau96I 804 GGACC G^G[ACGT]CC
SmlI 553 CTCAAG C^T[CT][GA]AG
SspBI 226 TGTACA T^GTACA
XhoII 207 GGATCT [GA]^GATC[CT]
XmnI 331 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 477 GTCTAC GT^[AC][GT]AC
698 GTCGAC
AflIII 223 ACATGT A^C[GA][CT]GT
718 ACATGT
BbsI 88 GTCTTC GAAGAC(2/6)
922 GTCTTC
BfaI 139 CTAG C^TAG
244 CTAG
BpuAI 88 GTCTTC GAAGAC(2/6)
922 GTCTTC
BsiYI 356 CCTTTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
423 CCTCCAGTTGG
BslI 356 CCTTTGATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
423 CCTCCAGTTGG
BspLU11I 223 ACATGT A^CATGT
718 ACATGT
BsrI 451 ACTGG ACTGG(1/-1)
426 CCAGT
BstF5I 440 CATCC GGATG(2/0)
639 CATCC
EarI 613 CTCTTC CTCTTC(1/4)
826 CTCTTC
FokI 440 CATCC GGATG(9/13)
639 CATCC
Ksp632I 613 CTCTTC CTCTTC(1/4)
826 CTCTTC
MaeI 139 CTAG C^TAG
244 CTAG
MseI 902 TTAA T^TAA
928 TTAA
MspA1I 434 CAGCTG C[AC]G^C[GT]G
493 CAGCTG
MwoI 316 GCCTTGGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
580 GCAAATGAGGC
NspI 223 ACATGT [GA]CATG^[CT]
718 ACATGT
PvuII 434 CAGCTG CAG^CTG
493 CAGCTG
TaqI 57 TCGA T^CGA
699 TCGA
TfiI 836 GAATC G^A[AT]TC
855 GAATC
Tru9I 902 TTAA T^TAA
928 TTAA
TthHB8I 57 TCGA T^CGA
699 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AfaI AluI BbvI BpmI BsaJI
BsmAI BsmFI BssKI BstNI DdeI
DpnII EcoRII EcoT14I Fnu4HI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MvaI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI StyI
TseI Tsp45I Tsp509I TspRI