Restriction map for
pFN21AE1206
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
BspDI BspEI BspLU11I BsrBI BsrDI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DrdI Eam1105I EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
TfiI Tru9I TspRI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcsI 189 GAATTC [GA]^AATT[CT]
AluI 109 AGCT AG^CT
AlwI 282 GATCC GGATC(4/5)
ApoI 189 GAATTC [GA]^AATT[CT]
AvaII 144 GGTCC G^G[AT]CC
BalI 320 TGGCCA TGG^CCA
BanII 39 GGGCTC G[GA]GC[CT]^C
BmyI 39 GGGCTC G[AGT]GC[ACT]^C
BpmI 273 CTCCAG CTGGAG(16/14)
BsiEI 290 CGGCCG CG[GA][CT]^CG
Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 125 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BsrFI 157 ACCGGC [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
BstXI 170 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 281 AGATCC [GA]^GATC[CT]
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
Cfr13I 144 GGTCC G^G[ACGT]CC
DpnII 282 GATC ^GATC
DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCATGG C^C[GA][CT]GG
EagI 290 CGGCCG C^GGCCG
EclXI 290 CGGCCG C^GGCCG
Eco52I 290 CGGCCG C^GGCCG
EcoRI 189 GAATTC G^AATTC
HapII 158 CCGG C^CGG
HpaII 158 CCGG C^CGG
MboI 282 GATC ^GATC
MboII 69 GAAGA GAAGA(8/7)
MflI 281 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MslI 117 CATCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 158 CCGG C^CGG
NcoI 176 CCATGG C^CATGG
NdeII 282 GATC ^GATC
NlaIV 40 GGCTCC GG[ACGT]^[ACGT]CC
RcaI 185 TCATGA T^CATGA
Sau3AI 282 GATC ^GATC
Sau96I 144 GGTCC G^G[ACGT]CC
SspBI 125 TGTACA T^GTACA
Tsp509I 190 AATT ^AATT
XhoII 281 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
Aor51HI 257 AGCGCT AGC^GCT
370 AGCGCT
BspMI 141 GCAGGT ACCTGC(4/8)
223 GCAGGT
EaeI 290 CGGCCG [CT]^GGCC[GA]
320 TGGCCA
Eco47III 257 AGCGCT AGC^GCT
370 AGCGCT
EcoT14I 170 CCAAGG C^C[AT][AT]GG
176 CCATGG
FauI 25 CCCGC CCCGC(4/6)
236 CCCGC
HaeII 257 AGCGCT [GA]GCGC^[CT]
370 AGCGCT
MaeIII 55 GTGAC ^GT[ACGT]AC
355 GTCAC
NlaIII 177 CATG CATG^
186 CATG
SfaNI 182 GCATC GCATC(5/9)
218 GCATC
StyI 170 CCAAGG C^C[AT][AT]GG
176 CCATGG
TaqI 212 TCGA T^CGA
262 TCGA
Tsp45I 55 GTGAC ^GT[GC]AC
355 GTCAC
TthHB8I 212 TCGA T^CGA
262 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AfaI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I CfoI
DdeI EcoRII Fnu4HI HaeIII HhaI
HinP1I HphI ItaI MnlI MvaI
MwoI RsaI ScrFI TseI