Restriction map for
pFN21AE6606
##################################
# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT14I FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 509 GGTACC G^GTACC
Alw44I 68 GTGCAC G^TGCAC
AlwNI 8 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 100 GGGCCC GGGCC^C
ApaLI 68 GTGCAC G^TGCAC
Asp700I 916 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 509 GGTACC G^GTACC
AvaI 163 CCCGAG C^[CT]CG[GA]G
BalI 119 TGGCCA TGG^CCA
BbsI 469 GAAGAC GAAGAC(2/6)
BcgI 165 CGAGAAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 620 TGATCA T^GATCA
BcnI 29 CCCGG CC^[GC]GG
BlpI 979 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTGAGC GC^T[ACGT]AGC
BpuAI 469 GAAGAC GAAGAC(2/6)
BsaWI 200 TCCGGT [AT]^CCGG[AT]
BsmFI 536 GGGAC GGGAC(10/14)
BsoBI 163 CCCGAG C^[CT]CG[GA]G
BspLU11I 647 ACATGT A^CATGT
BspMI 389 GCAGGT ACCTGC(4/8)
BsrDI 783 CATTGC GCAATG(2/0)
BstEII 606 GGTCACC G^GT[ACGT]ACC
BstPI 606 GGTCACC G^GT[ACGT]ACC
CelII 979 GCTGAGC GC^T[ACGT]AGC
CfoI 556 GCGC GCG^C
DraIII 525 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 598 CTCTTC CTCTTC(1/4)
EcoNI 564 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 606 GGTCACC G^GT[ACGT]ACC
EcoT22I 792 ATGCAT ATGCA^T
FauI 217 CCCGC CCCGC(4/6)
FbaI 620 TGATCA T^GATCA
HhaI 556 GCGC GCG^C
HinP1I 556 GCGC G^CGC
KpnI 509 GGTACC GGTAC^C
Ksp632I 598 CTCTTC CTCTTC(1/4)
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
NciI 29 CCCGG CC^[GC]GG
NsiI 792 ATGCAT ATGCA^T
PvuII 8 CAGCTG CAG^CTG
SacI 982 GAGCTC GAGCT^C
XbaI 499 TCTAGA T^CTAGA
XmnI 916 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 273 GTAC GT^AC
510 GTAC
AflIII 647 ACATGT A^C[GA][CT]GT
900 ACGTGT
AluI 9 AGCT AG^CT
983 AGCT
AlwI 109 GATCC GGATC(4/5)
803 GGATC
AspHI 68 GTGCAC G[AT]GC[AT]^C
982 GAGCTC
AvaII 392 GGTCC G^G[AT]CC
537 GGACC
BanI 361 GGCACC G^G[CT][GA]CC
509 GGTACC
BanII 100 GGGCCC G[GA]GC[CT]^C
982 GAGCTC
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
175 GCTGAGG
BfaI 433 CTAG C^TAG
500 CTAG
BpmI 487 CTGGAG CTGGAG(16/14)
889 CTGGAG
BsiHKAI 68 GTGCAC G[AT]GC[AT]^C
982 GAGCTC
BstF5I 408 CATCC GGATG(2/0)
480 CATCC
BstNI 327 CCTGG CC^[AT]GG
351 CCAGG
Cac8I 33 GCCAGC GC[ACGT]^[ACGT]GC
558 GCCTGC
DsaI 122 CCACGG C^C[GA][CT]GG
611 CCACGG
EcoRII 327 CCTGG ^CC[AT]GG
351 CCAGG
FokI 408 CATCC GGATG(9/13)
480 CATCC
HphI 160 TCACC GGTGA(8/7)
608 TCACC
MaeI 433 CTAG C^TAG
500 CTAG
MaeII 142 ACGT A^CGT
900 ACGT
MspA1I 8 CAGCTG C[AC]G^C[GT]G
369 CCGCTG
MvaI 327 CCTGG CC^[AT]GG
351 CCAGG
MwoI 311 GCAGGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
362 GCACCTACCGC
NspI 647 ACATGT [GA]CATG^[CT]
975 ACATGC
PpuMI 391 AGGTCCT [GA]G^G[AT]CC[CT]
536 GGGACCC
RsaI 273 GTAC GT^AC
510 GTAC
Tsp45I 159 GTCAC ^GT[GC]AC
607 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI BbvI BmyI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI Cfr13I DdeI
DpnII DraII EaeI EcoO109I Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MaeIII MboI MboII MnlI MslI
MspI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
Tsp509I TspRI