Restriction map for
pFN21AE1215
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AfaI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BclI
BfaI BfrI BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BsrI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I MaeI
MaeII MamI MfeI MluI MroI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SapI ScaI
SexAI SfaNI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccI 171 GTCGAC GT^[AC][GT]AC
AgeI 34 ACCGGT A^CCGGT
AlwI 186 GATCC GGATC(4/5)
BalI 62 TGGCCA TGG^CCA
BanI 133 GGCACC G^G[CT][GA]CC
BanII 307 GGGCTC G[GA]GC[CT]^C
BcgI 173 CGACTGAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 318 GGATAC GTATCC(6/5)
BcnI 130 CCCGG CC^[GC]GG
BglII 243 AGATCT A^GATCT
BlpI 343 GCTAAGC GC^T[ACGT]AGC
BmyI 307 GGGCTC G[AGT]GC[ACT]^C
Bpu1102I 343 GCTAAGC GC^T[ACGT]AGC
BsaWI 34 ACCGGT [AT]^CCGG[AT]
BsgI 20 CTGCAC GTGCAG(16/14)
BsmFI 128 GTCCC GGGAC(10/14)
BsmI 389 GCATTC GAATGC(1/-1)
Bsp1286I 307 GGGCTC G[AGT]GC[ACT]^C
BssHII 86 GCGCGC G^CGCGC
BstF5I 336 CATCC GGATG(2/0)
BstNI 210 CCTGG CC^[AT]GG
BstYI 243 AGATCT [GA]^GATC[CT]
CelII 343 GCTAAGC GC^T[ACGT]AGC
EcoNI 276 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 210 CCTGG ^CC[AT]GG
EcoT14I 365 CCAAGG C^C[AT][AT]GG
FauI 5 CCCGC CCCGC(4/6)
FokI 336 CATCC GGATG(9/13)
HaeII 45 AGCGCC [GA]GCGC^[CT]
HincII 171 GTCGAC GT[CT]^[GA]AC
HindII 171 GTCGAC GT[CT]^[GA]AC
HinfI 153 GATTC G^A[ACGT]TC
HphI 267 GGTGA GGTGA(8/7)
KspI 263 CCGCGG CCGC^GG
MaeIII 352 GTGAC ^GT[ACGT]AC
MboII 108 GAAGA GAAGA(8/7)
MflI 243 AGATCT [GA]^GATC[CT]
MluNI 62 TGGCCA TGG^CCA
MscI 62 TGGCCA TGG^CCA
MvaI 210 CCTGG CC^[AT]GG
NaeI 90 GCCGGC GCC^GGC
NciI 130 CCCGG CC^[GC]GG
NgoMIV 90 GCCGGC G^CCGGC
NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC
PflMI 204 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 34 ACCGGT A^CCGGT
PstI 277 CTGCAG CTGCA^G
PvuII 58 CAGCTG CAG^CTG
SacII 263 CCGCGG CCGC^GG
SalI 171 GTCGAC G^TCGAC
SbfI 276 CCTGCAGG CCTGCA^GG
SfcI 277 CTGCAG C^T[GA][CT]AG
SfiI 285 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 276 CCTGCAGG CCTGCA^GG
StyI 365 CCAAGG C^C[AT][AT]GG
TaqI 172 TCGA T^CGA
TfiI 153 GATTC G^A[AT]TC
Tsp45I 352 GTGAC ^GT[GC]AC
TthHB8I 172 TCGA T^CGA
Van91I 204 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 243 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvI 57 GCAGC GCAGC(8/12)
180 GCTGC
BglI 64 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
286 GCCTGCGAGGC
BssKI 130 CCCGG ^CC[ACGT]GG
210 CCTGG
Cfr13I 3 GGCCC G^G[ACGT]CC
273 GGCCC
DpnII 186 GATC ^GATC
244 GATC
DsaI 137 CCGTGG C^C[GA][CT]GG
263 CCGCGG
EaeI 62 TGGCCA [CT]^GGCC[GA]
92 CGGCCA
MboI 186 GATC ^GATC
244 GATC
MspA1I 58 CAGCTG C[AC]G^C[GT]G
263 CCGCGG
NdeII 186 GATC ^GATC
244 GATC
NlaIII 340 CATG CATG^
360 CATG
Sau3AI 186 GATC ^GATC
244 GATC
Sau96I 3 GGCCC G^G[ACGT]CC
273 GGCCC
ScrFI 130 CCCGG CC^[ACGT]GG
210 CCTGG
SgrAI 33 CACCGGTG C[GA]^CCGG[CT]G
96 CACCGGCG
StuI 284 AGGCCT AGG^CCT
293 AGGCCT
TseI 57 GCAGC G^C[AT]GC
180 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI AluI BsaJI BsiYI
BslI BsrFI BstUI Cac8I CfoI
Cfr10I DdeI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MnlI
MspI MvnI MwoI