Restriction map for
pFN21AE1214
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AclI AcsI AcyI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI KasI KpnI
Ksp632I MaeI MaeII MamI MfeI
MseI MslI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI Tru9I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 171 GTCTAC GT^[AC][GT]AC
AccIII 188 TCCGGA T^CCGGA
AfaI 8 GTAC GT^AC
AflIII 348 ACGCGT A^C[GA][CT]GT
AlwI 153 GATCC GGATC(4/5)
AlwNI 205 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 85 AGCGCT AGC^GCT
BalI 62 TGGCCA TGG^CCA
BanI 133 GGCACC G^G[CT][GA]CC
BcgI 15 GCAGACGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 188 TCCGGA [AT]^CCGG[AT]
BseAI 188 TCCGGA T^CCGGA
BsiYI 91 CCGGCCACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 91 CCGGCCACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 389 GCATTC GAATGC(1/-1)
BspEI 188 TCCGGA T^CCGGA
BspMI 245 ACCTGC ACCTGC(4/8)
BstF5I 372 CATCC GGATG(2/0)
BstYI 152 AGATCC [GA]^GATC[CT]
DpnII 153 GATC ^GATC
Eco47III 85 AGCGCT AGC^GCT
FauI 383 CCCGC CCCGC(4/6)
FokI 372 CATCC GGATG(9/13)
HphI 267 GGTGA GGTGA(8/7)
KspI 263 CCGCGG CCGC^GG
MaeIII 352 GTGAC ^GT[ACGT]AC
MboI 153 GATC ^GATC
MboII 108 GAAGA GAAGA(8/7)
MflI 152 AGATCC [GA]^GATC[CT]
MluI 348 ACGCGT A^CGCGT
MluNI 62 TGGCCA TGG^CCA
MroI 188 TCCGGA T^CCGGA
MscI 62 TGGCCA TGG^CCA
NdeII 153 GATC ^GATC
NlaIV 133 GGCACC GG[ACGT]^[ACGT]CC
PstI 277 CTGCAG CTGCA^G
PvuII 58 CAGCTG CAG^CTG
RsaI 8 GTAC GT^AC
SacII 263 CCGCGG CCGC^GG
Sau3AI 153 GATC ^GATC
SfcI 277 CTGCAG C^T[GA][CT]AG
SfiI 285 GGCCTGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 314 TCGA T^CGA
Tsp45I 352 GTGAC ^GT[GC]AC
TthHB8I 314 TCGA T^CGA
XhoII 152 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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BglI 64 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
286 GCCTGCGAGGC
BssKI 130 CCTGG ^CC[ACGT]GG
210 CCTGG
BstNI 130 CCTGG CC^[AT]GG
210 CCTGG
EaeI 62 TGGCCA [CT]^GGCC[GA]
92 CGGCCA
EcoRII 130 CCTGG ^CC[AT]GG
210 CCTGG
HapII 91 CCGG C^CGG
189 CCGG
HpaII 91 CCGG C^CGG
189 CCGG
MspA1I 58 CAGCTG C[AC]G^C[GT]G
263 CCGCGG
MspI 91 CCGG C^CGG
189 CCGG
MvaI 130 CCTGG CC^[AT]GG
210 CCTGG
NlaIII 340 CATG CATG^
360 CATG
ScrFI 130 CCTGG CC^[ACGT]GG
210 CCTGG
StuI 284 AGGCCT AGG^CCT
293 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AccII AciI AluI BbvI BsaJI
BstUI Cac8I CfoI DdeI DsaI
Fnu4HI HaeII HaeIII HhaI HinP1I
ItaI MnlI MvnI MwoI TseI