Restriction map for
pFN21AE9604
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstYI BstZ17I
CelII ClaI CpoI DpnII DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MaeIII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MunI NcoI NdeI
NdeII NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI Sau3AI SbfI ScaI SexAI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcyI 549 GGCGCC G[GA]^CG[CT]C
AocI 352 CCTAAGG CC^T[ACGT]AGG
Aor51HI 192 AGCGCT AGC^GCT
ApaI 109 GGGCCC GGGCC^C
AspHI 124 GAGCTC G[AT]GC[AT]^C
BbeI 549 GGCGCC GGCGC^C
BcnI 114 CCCGG CC^[GC]GG
BpmI 494 CTGGAG CTGGAG(16/14)
BsaHI 549 GGCGCC G[GA]^CG[CT]C
BsaI 247 GGTCTC GGTCTC(1/5)
BsgI 280 GTGCAG GTGCAG(16/14)
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
BspMI 82 GCAGGT ACCTGC(4/8)
Bsu36I 352 CCTAAGG CC^T[ACGT]AGG
DdeI 353 CTAAG C^T[ACGT]AG
DraII 109 GGGCCCC [GA]G^G[ACGT]CC[CT]
EarI 444 GAAGAG CTCTTC(1/4)
Eco47III 192 AGCGCT AGC^GCT
Eco81I 352 CCTAAGG CC^T[ACGT]AGG
EcoO109I 109 GGGCCCC [GA]G^G[ACGT]CC[CT]
Hin1I 549 GGCGCC G[GA]^CG[CT]C
HinfI 51 GACTC G^A[ACGT]TC
HphI 261 GGTGA GGTGA(8/7)
KasI 549 GGCGCC G^GCGCC
Ksp632I 444 GAAGAG CTCTTC(1/4)
MaeII 215 ACGT A^CGT
MseI 581 TTAA T^TAA
MslI 273 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 480 GCCGGC GCC^GGC
NarI 549 GGCGCC GG^CGCC
NciI 114 CCCGG CC^[GC]GG
NgoMIV 480 GCCGGC G^CCGGC
PleI 51 GACTC GAGTC(4/5)
PvuII 488 CAGCTG CAG^CTG
SacI 124 GAGCTC GAGCT^C
SapI 444 GAAGAGC GCTCTTC(1/4)
SfaNI 236 GCATC GCATC(5/9)
SfoI 549 GGCGCC GGC^GCC
SgrAI 294 CACCGGCG C[GA]^CCGG[CT]G
Tru9I 581 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 200 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
488 CAGCTGCTG
BbsI 48 GAAGAC GAAGAC(2/6)
456 GAAGAC
BpuAI 48 GAAGAC GAAGAC(2/6)
456 GAAGAC
BsmAI 7 GAGAC GTCTC(1/5)
248 GTCTC
BsrBI 179 CCGCTC CCGCTC(-3/-3)
398 GAGCGG
BsrFI 295 ACCGGC [GA]^CCGG[CT]
480 GCCGGC
BstNI 258 CCTGG CC^[AT]GG
276 CCTGG
Cfr10I 295 ACCGGC [GA]^CCGG[CT]
480 GCCGGC
EcoRII 258 CCTGG ^CC[AT]GG
276 CCTGG
FauI 25 CCCGC CCCGC(4/6)
71 CCCGC
HgaI 103 GCGTC GACGC(5/10)
301 GCGTC
MnlI 149 CCTC CCTC(7/6)
335 CCTC
MvaI 258 CCTGG CC^[AT]GG
276 CCTGG
PstI 80 CTGCAG CTGCA^G
485 CTGCAG
SfcI 80 CTGCAG C^T[GA][CT]AG
485 CTGCAG
SmlI 184 CTCAAG C^T[CT][GA]AG
250 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AccII AciI AluI BanI BanII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstUI Cac8I CfoI
Cfr13I EaeI EcoT14I Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI MboII MspA1I MspI MvnI
MwoI NlaIV Sau96I ScrFI StyI
TseI