Restriction map for
pFN21AE1400
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccIII AcsI
AfaI AflIII AgeI AhdI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BclI BfaI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr13I ClaI CpoI DpnII DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeIII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MboI MboII MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI TseI
Tsp509I TspRI Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AclI 193 AACGTT AA^CGTT
AflII 175 CTTAAG C^TTAAG
AspI 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 164 CTCGAG C^[CT]CG[GA]G
BcnI 69 CCGGG CC^[GC]GG
BfrI 175 CTTAAG C^TTAAG
BlpI 46 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 46 GCTAAGC GC^T[ACGT]AGC
BsaWI 142 TCCGGT [AT]^CCGG[AT]
BseRI 157 GAGGAG GAGGAG(10/8)
BsiYI 54 CCATCGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 54 CCATCGTAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 203 GAGACG CGTCTC(1/5)
BsoBI 164 CTCGAG C^[CT]CG[GA]G
BsrFI 96 ACCGGC [GA]^CCGG[CT]
BstNI 81 CCAGG CC^[AT]GG
Cac8I 225 GCACGC GC[ACGT]^[ACGT]GC
CelII 46 GCTAAGC GC^T[ACGT]AGC
Cfr10I 96 ACCGGC [GA]^CCGG[CT]
DsaI 251 CCATGG C^C[GA][CT]GG
EcoRII 81 CCAGG ^CC[AT]GG
EcoT14I 251 CCATGG C^C[AT][AT]GG
HaeII 50 AGCGCC [GA]GCGC^[CT]
HinfI 162 GACTC G^A[ACGT]TC
HphI 212 TCACC GGTGA(8/7)
MseI 176 TTAA T^TAA
MslI 246 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 81 CCAGG CC^[AT]GG
NciI 69 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
NlaIII 252 CATG CATG^
PaeR7I 164 CTCGAG C^TCGAG
PflFI 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 162 GACTC GAGTC(4/5)
Psp1406I 193 AACGTT AA^CGTT
StyI 251 CCATGG C^C[AT][AT]GG
Tru9I 176 TTAA T^TAA
Tth111I 205 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 164 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 10 CGCG CG^CG
121 CGCG
AcyI 127 GGCGTC G[GA]^CG[CT]C
205 GACGCC
AspHI 64 GTGCTC G[AT]GC[AT]^C
223 GAGCAC
BmyI 64 GTGCTC G[AGT]GC[ACT]^C
223 GAGCAC
BsaHI 127 GGCGTC G[GA]^CG[CT]C
205 GACGCC
BsaJI 81 CCAGGG C^C[ACGT][ACGT]GG
251 CCATGG
BsiHKAI 64 GTGCTC G[AT]GC[AT]^C
223 GAGCAC
BsmAI 160 GAGAC GTCTC(1/5)
203 GAGAC
Bsp1286I 64 GTGCTC G[AGT]GC[ACT]^C
223 GAGCAC
BssKI 69 CCGGG ^CC[ACGT]GG
81 CCAGG
BstF5I 78 CATCC GGATG(2/0)
255 GGATG
BstUI 10 CGCG CG^CG
121 CGCG
DdeI 47 CTAAG C^T[ACGT]AG
186 CTTAG
Fnu4HI 11 GCGGC GC^[ACGT]GC
305 GCGGC
FokI 78 CATCC GGATG(9/13)
255 GGATG
Hin1I 127 GGCGTC G[GA]^CG[CT]C
205 GACGCC
ItaI 11 GCGGC GC^[ACGT]GC
305 GCGGC
MaeII 194 ACGT A^CGT
276 ACGT
MaeIII 211 GTCAC ^GT[ACGT]AC
244 GTCAC
MnlI 157 GAGG CCTC(7/6)
167 GAGG
MvnI 10 CGCG CG^CG
121 CGCG
ScrFI 69 CCGGG CC^[ACGT]GG
81 CCAGG
SmlI 164 CTCGAG C^T[CT][GA]AG
175 CTTAAG
TaqI 165 TCGA T^CGA
201 TCGA
Tsp45I 211 GTCAC ^GT[GC]AC
244 GTCAC
TthHB8I 165 TCGA T^CGA
201 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
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AciI CfoI HapII HgaI HhaI
HinP1I HpaII MspI MwoI