Restriction map for
pFN21AE1710
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII AccI AccII AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstBI BstEII
BstF5I BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI Ksp632I KspI
MamI MfeI MflI MluI MluNI
MroI MscI MslI MunI MvnI
NaeI NcoI NdeI NgoMIV NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 301 GGTACC G^GTACC
AclI 282 AACGTT AA^CGTT
AfaI 302 GTAC GT^AC
Asp718I 301 GGTACC G^GTACC
AvaI 312 CTCGGG C^[CT]CG[GA]G
BbeI 364 GGCGCC GGCGC^C
BcnI 361 CCCGG CC^[GC]GG
BglI 424 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 304 ACCGGA [AT]^CCGG[AT]
BseRI 156 CTCCTC GAGGAG(10/8)
BsiEI 209 CGGCCG CG[GA][CT]^CG
BsoBI 312 CTCGGG C^[CT]CG[GA]G
BspMI 78 ACCTGC ACCTGC(4/8)
BstAPI 394 GCAACGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 267 CCTGG CC^[AT]GG
Cfr13I 118 GGCCC G^G[ACGT]CC
DpnII 138 GATC ^GATC
EagI 209 CGGCCG C^GGCCG
EclXI 209 CGGCCG C^GGCCG
Eco52I 209 CGGCCG C^GGCCG
EcoRII 267 CCTGG ^CC[AT]GG
HphI 594 GGTGA GGTGA(8/7)
KasI 364 GGCGCC G^GCGCC
KpnI 301 GGTACC GGTAC^C
MaeIII 595 GTGAC ^GT[ACGT]AC
MboI 138 GATC ^GATC
MboII 231 GAAGA GAAGA(8/7)
MspA1I 113 CCGCTG C[AC]G^C[GT]G
MvaI 267 CCTGG CC^[AT]GG
NarI 364 GGCGCC GG^CGCC
NciI 361 CCCGG CC^[GC]GG
NdeII 138 GATC ^GATC
NheI 433 GCTAGC G^CTAGC
NotI 208 GCGGCCGC GC^GGCCGC
PleI 453 GACTC GAGTC(4/5)
Psp1406I 282 AACGTT AA^CGTT
PstI 89 CTGCAG CTGCA^G
RsaI 302 GTAC GT^AC
Sau3AI 138 GATC ^GATC
Sau96I 118 GGCCC G^G[ACGT]CC
SfaNI 403 GCATC GCATC(5/9)
SfcI 89 CTGCAG C^T[GA][CT]AG
SfoI 364 GGCGCC GGC^GCC
TfiI 501 GAATC G^A[AT]TC
Tsp45I 595 GTGAC ^GT[GC]AC
TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 339 GGCGTC G[GA]^CG[CT]C
364 GGCGCC
BfaI 434 CTAG C^TAG
590 CTAG
BsaHI 339 GGCGTC G[GA]^CG[CT]C
364 GGCGCC
BsgI 142 GTGCAG GTGCAG(16/14)
289 GTGCAG
BsiYI 52 CCTTTAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
584 CCAAGGCTAGG
BslI 52 CCTTTAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
584 CCAAGGCTAGG
BssKI 267 CCTGG ^CC[ACGT]GG
361 CCCGG
CfoI 202 GCGC GCG^C
365 GCGC
DsaI 347 CCGTGG C^C[GA][CT]GG
425 CCACGG
EaeI 209 CGGCCG [CT]^GGCC[GA]
581 CGGCCA
EcoT14I 575 CCAAGG C^C[AT][AT]GG
584 CCAAGG
HaeII 201 GGCGCT [GA]GCGC^[CT]
364 GGCGCC
HapII 305 CCGG C^CGG
362 CCGG
HgaI 340 GCGTC GACGC(5/10)
446 GCGTC
HhaI 202 GCGC GCG^C
365 GCGC
Hin1I 339 GGCGTC G[GA]^CG[CT]C
364 GGCGCC
HinP1I 202 GCGC G^CGC
365 GCGC
HinfI 453 GACTC G^A[ACGT]TC
501 GAATC
HpaII 305 CCGG C^CGG
362 CCGG
MaeI 434 CTAG C^TAG
590 CTAG
MaeII 224 ACGT A^CGT
283 ACGT
MspI 305 CCGG C^CGG
362 CCGG
ScrFI 267 CCTGG CC^[ACGT]GG
361 CCCGG
StyI 575 CCAAGG C^C[AT][AT]GG
584 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AciI AluI BanI BbvI BsaJI
Cac8I DdeI FauI Fnu4HI HaeIII
ItaI MnlI MseI MwoI NlaIV
Tru9I TseI