##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        ItaI        
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AluI          140  AGCT                 AG^CT
AlwI          562  GATCC                GGATC(4/5)
AseI          472  ATTAAT               AT^TAAT
AsnI          472  ATTAAT               AT^TAAT
AvaII         580  GGTCC                G^G[AT]CC
BcnI          243  CCGGG                CC^[GC]GG
BseRI         455  GAGGAG               GAGGAG(10/8)
BsiYI         202  CCTCTTTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          202  CCTCTTTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         246  GGGAC                GGGAC(10/14)
BstAPI        460  GCATTAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr13I        580  GGTCC                G^G[ACGT]CC
EarI           31  GAAGAG               CTCTTC(1/4)
HapII         243  CCGG                 C^CGG
HinfI         309  GAATC                G^A[ACGT]TC
HpaII         243  CCGG                 C^CGG
Ksp632I        31  GAAGAG               CTCTTC(1/4)
MaeII         642  ACGT                 A^CGT
MspI          243  CCGG                 C^CGG
MwoI          460  GCATTAGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          243  CCGGG                CC^[GC]GG
NlaIV         349  GGAACC               GG[ACGT]^[ACGT]CC
NspI          600  ACATGC               [GA]CATG^[CT]
PshBI         472  ATTAAT               AT^TAAT
Sau96I        580  GGTCC                G^G[ACGT]CC
SfaNI         661  GATGC                GCATC(5/9)
SmlI          649  CTTGAG               C^T[CT][GA]AG
SspI          424  AATATT               AAT^ATT
TfiI          309  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AciI          209  GCGG                 CCGC(-3/-1)
              217  GCGG                
BciVI          42  GGATAC               GTATCC(6/5)
              284  GGATAC              
BstF5I        574  CATCC                GGATG(2/0)
                3  GGATG               
FokI          574  CATCC                GGATG(9/13)
                3  GGATG               
NlaIII        441  CATG                 CATG^
              601  CATG                
ScaI           35  AGTACT               AGT^ACT
               66  AGTACT              
TspRI         252  CAGTG                CA[GC]TG[ACGT][ACGT]^
              273  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AcsI        AfaI        ApoI        BsaJI       BssKI       
BstNI       DdeI        DpnII       EcoRII      HphI        
MboI        MboII       MnlI        MseI        MvaI        
NdeII       RsaI        Sau3AI      ScrFI       Tru9I       
Tsp509I