Restriction map for
pFN21AE1955
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Aor51HI AscI
AseI AsnI Asp700I Asp718I AvaII
AvrII BalI BamHI BbsI BbvCI
BciVI BclI BfrI BlnI BpuAI
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I BspMI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraIII Eco47III EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
TaqI TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AhdI 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1102 GTGCAC G^TGCAC
AocI 736 CCTGAGG CC^T[ACGT]AGG
ApaI 67 GGGCCC GGGCC^C
ApaLI 1102 GTGCAC G^TGCAC
AspEI 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1102 GTGCAC G[AT]GC[AT]^C
AspI 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1109 TGCGCA TGC^GCA
BbrPI 1105 CACGTG CAC^GTG
BfaI 938 CTAG C^TAG
BglI 1034 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 245 AGATCT A^GATCT
BlpI 984 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 984 GCTGAGC GC^T[ACGT]AGC
BsaAI 1105 CACGTG [CT]AC^GT[GA]
BsaI 1188 GGTCTC GGTCTC(1/5)
BsiHKAI 1102 GTGCAC G[AT]GC[AT]^C
BsmBI 1046 GAGACG CGTCTC(1/5)
Bsp1407I 416 TGTACA T^GTACA
BspHI 644 TCATGA T^CATGA
BsrBI 541 CCGCTC CCGCTC(-3/-3)
BsrDI 1290 CATTGC GCAATG(2/0)
BsrGI 416 TGTACA T^GTACA
BssHII 605 GCGCGC G^CGCGC
Bsu36I 736 CCTGAGG CC^T[ACGT]AGG
CelII 984 GCTGAGC GC^T[ACGT]AGC
DraI 799 TTTAAA TTT^AAA
DraII 67 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 1142 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 101 CCGCGG C^C[GA][CT]GG
Eam1105I 448 GACGGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 400 CTCTTC CTCTTC(1/4)
Eco81I 736 CCTGAGG CC^T[ACGT]AGG
EcoO109I 67 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 763 GAATTC G^AATTC
FspI 1109 TGCGCA TGC^GCA
Ksp632I 400 CTCTTC CTCTTC(1/4)
KspI 101 CCGCGG CCGC^GG
MaeI 938 CTAG C^TAG
MseI 800 TTAA T^TAA
NaeI 1133 GCCGGC GCC^GGC
NgoMIV 1133 GCCGGC G^CCGGC
NotI 103 GCGGCCGC GC^GGCCGC
PflFI 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1105 CACGTG CAC^GTG
PmlI 1105 CACGTG CAC^GTG
PshAI 383 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 644 TCATGA T^CATGA
SacII 101 CCGCGG CCGC^GG
SbfI 1232 CCTGCAGG CCTGCA^GG
SmlI 211 CTCAAG C^T[CT][GA]AG
Sse8387I 1232 CCTGCAGG CCTGCA^GG
SspBI 416 TGTACA T^GTACA
StuI 1032 AGGCCT AGG^CCT
TfiI 732 GATTC G^A[AT]TC
Tru9I 800 TTAA T^TAA
Tth111I 637 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 763 GAATTC [GA]^AATT[CT]
919 GAATTT
ApoI 763 GAATTC [GA]^AATT[CT]
919 GAATTT
BanI 529 GGCGCC G^G[CT][GA]CC
1136 GGCGCC
BbeI 529 GGCGCC GGCGC^C
1136 GGCGCC
BcgI 351 GCAGCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
493 GCAGCCAGCTCG
BpmI 1128 CTCCAG CTGGAG(16/14)
1191 CTCCAG
BseRI 186 CTCCTC GAGGAG(10/8)
331 GAGGAG
BsiEI 104 CGGCCG CG[GA][CT]^CG
150 CGGCCG
BsmI 1330 GAATGC GAATGC(1/-1)
1359 GAATGC
BstAPI 56 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
348 GCAGCAGCTGC
EagI 104 CGGCCG C^GGCCG
150 CGGCCG
EclXI 104 CGGCCG C^GGCCG
150 CGGCCG
Eco52I 104 CGGCCG C^GGCCG
150 CGGCCG
EcoT14I 1298 CCAAGG C^C[AT][AT]GG
1388 CCTTGG
KasI 529 GGCGCC G^GCGCC
1136 GGCGCC
MslI 639 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1354 CACATGAATG
NarI 529 GGCGCC GG^CGCC
1136 GGCGCC
PflMI 1173 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1263 CCAAGACCTGG
SexAI 659 ACCTGGT A^CC[AT]GGT
1117 ACCAGGT
SfoI 529 GGCGCC GGC^GCC
1136 GGCGCC
SmaI 376 CCCGGG CCC^GGG
525 CCCGGG
StyI 1298 CCAAGG C^C[AT][AT]GG
1388 CCTTGG
Van91I 1173 CCAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1263 CCAAGACCTGG
XmaCI 376 CCCGGG C^CCGGG
525 CCCGGG
XmaI 376 CCCGGG C^CCGGG
525 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI BanII BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I Tsp509I TspRI XhoII