Restriction map for
pFN21AE1858
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BglII BpmI BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
HphI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 432 TCCGGA T^CCGGA
AflII 634 CTTAAG C^TTAAG
AlwI 882 GGATC GGATC(4/5)
AlwNI 211 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1097 CCTCAGG CC^T[ACGT]AGG
Asp700I 190 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 870 CCTAGG C^CTAGG
BbsI 387 GAAGAC GAAGAC(2/6)
BfrI 634 CTTAAG C^TTAAG
BlnI 870 CCTAGG C^CTAGG
BpuAI 387 GAAGAC GAAGAC(2/6)
BsaWI 432 TCCGGA [AT]^CCGG[AT]
BseAI 432 TCCGGA T^CCGGA
BseRI 670 GAGGAG GAGGAG(10/8)
BsgI 877 GTGCAG GTGCAG(16/14)
BsmFI 133 GGGAC GGGAC(10/14)
BspEI 432 TCCGGA T^CCGGA
BspHI 524 TCATGA T^CATGA
BstYI 882 GGATCT [GA]^GATC[CT]
Bsu36I 1097 CCTCAGG CC^T[ACGT]AGG
DraII 629 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 1097 CCTCAGG CC^T[ACGT]AGG
EcoNI 658 CCTTACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 629 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 226 GATATC GAT^ATC
HgaI 244 GCGTC GACGC(5/10)
HincII 747 GTTGAC GT[CT]^[GA]AC
HindII 747 GTTGAC GT[CT]^[GA]AC
MaeII 237 ACGT A^CGT
MflI 882 GGATCT [GA]^GATC[CT]
MroI 432 TCCGGA T^CCGGA
MslI 1032 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 119 CCATGG C^CATGG
PpuMI 629 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 524 TCATGA T^CATGA
SmlI 634 CTTAAG C^T[CT][GA]AG
SphI 366 GCATGC GCATG^C
XhoII 882 GGATCT [GA]^GATC[CT]
XmnI 190 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 12 GAGCTC G[AT]GC[AT]^C
673 GAGCTC
BalI 116 TGGCCA TGG^CCA
347 TGGCCA
BanII 12 GAGCTC G[GA]GC[CT]^C
673 GAGCTC
BfaI 481 CTAG C^TAG
871 CTAG
BmyI 12 GAGCTC G[AGT]GC[ACT]^C
673 GAGCTC
BsiHKAI 12 GAGCTC G[AT]GC[AT]^C
673 GAGCTC
BsmI 219 GAATGC GAATGC(1/-1)
543 GAATGC
Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C
673 GAGCTC
DpnII 883 GATC ^GATC
907 GATC
DsaI 119 CCATGG C^C[GA][CT]GG
719 CCACGG
EaeI 116 TGGCCA [CT]^GGCC[GA]
347 TGGCCA
FauI 26 CCCGC CCCGC(4/6)
866 CCCGC
MaeI 481 CTAG C^TAG
871 CTAG
MaeIII 143 GTAAC ^GT[ACGT]AC
842 GTTAC
MboI 883 GATC ^GATC
907 GATC
MluNI 116 TGGCCA TGG^CCA
347 TGGCCA
MscI 116 TGGCCA TGG^CCA
347 TGGCCA
NdeI 332 CATATG CA^TATG
614 CATATG
NdeII 883 GATC ^GATC
907 GATC
NlaIV 133 GGGACC GG[ACGT]^[ACGT]CC
925 GGAGCC
SacI 12 GAGCTC GAGCT^C
673 GAGCTC
Sau3AI 883 GATC ^GATC
907 GATC
TfiI 206 GATTC G^A[AT]TC
786 GAATC
XcmI 119 CCATGGTTTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
821 CCAGGTTCAAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BlpI Bpu1102I BsaJI BsiYI
BslI BsrI BssKI BstF5I BstNI
Cac8I CelII Cfr13I DdeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MboII MnlI MseI MspA1I MspI
MvaI MwoI NlaIII NspI PleI
PvuII Sau96I ScrFI SfaNI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I