Restriction map for
pFN21AB9605
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AvrII BamHI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EarI Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI HphI
KpnI Ksp632I MaeII MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AhdI 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 323 GTGCAC G^TGCAC
AocI 855 CCTCAGG CC^T[ACGT]AGG
ApaI 953 GGGCCC GGGCC^C
ApaLI 323 GTGCAC G^TGCAC
AspEI 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 323 GTGCAC G[AT]GC[AT]^C
AspI 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 663 GGTCC G^G[AT]CC
AviII 830 TGCGCA TGC^GCA
BalI 389 TGGCCA TGG^CCA
BbsI 622 GTCTTC GAAGAC(2/6)
BbvCI 645 CCTCAGC CCTCAGC(-5/-2)
BcgI 192 CGAACAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1204 CTAG C^TAG
BpuAI 622 GTCTTC GAAGAC(2/6)
BsgI 135 CTGCAC GTGCAG(16/14)
BsiHKAI 323 GTGCAC G[AT]GC[AT]^C
BsiWI 974 CGTACG C^GTACG
BspMI 1178 ACCTGC ACCTGC(4/8)
BsrI 578 ACTGG ACTGG(1/-1)
BstYI 174 AGATCC [GA]^GATC[CT]
Bsu36I 855 CCTCAGG CC^T[ACGT]AGG
DrdI 523 GACTGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 855 CCTCAGG CC^T[ACGT]AGG
EcoT14I 386 CCATGG C^C[AT][AT]GG
FspI 830 TGCGCA TGC^GCA
MaeI 1204 CTAG C^TAG
MaeIII 53 GTGAC ^GT[ACGT]AC
MboII 623 TCTTC GAAGA(8/7)
MflI 174 AGATCC [GA]^GATC[CT]
MluNI 389 TGGCCA TGG^CCA
MscI 389 TGGCCA TGG^CCA
MslI 381 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 386 CCATGG C^CATGG
NspI 749 GCATGC [GA]CATG^[CT]
PflFI 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 242 CAGCTG CAG^CTG
SphI 749 GCATGC GCATG^C
StyI 386 CCATGG C^C[AT][AT]GG
TaqI 818 TCGA T^CGA
Tsp45I 53 GTGAC ^GT[GC]AC
Tth111I 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 818 TCGA T^CGA
XhoII 174 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwI 175 GATCC GGATC(4/5)
231 GATCC
AlwNI 239 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
242 CAGCTGCTG
BanII 120 GGGCTC G[GA]GC[CT]^C
953 GGGCCC
BpmI 801 CTCCAG CTGGAG(16/14)
58 CTGGAG
BsmAI 107 GAGAC GTCTC(1/5)
1195 GAGAC
BsmBI 107 GAGACG CGTCTC(1/5)
1195 GAGACG
BstAPI 238 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
241 GCAGCTGCTGC
EagI 152 CGGCCG C^GGCCG
1097 CGGCCG
EclXI 152 CGGCCG C^GGCCG
1097 CGGCCG
Eco52I 152 CGGCCG C^GGCCG
1097 CGGCCG
HinfI 112 GACTC G^A[ACGT]TC
400 GACTC
NaeI 990 GCCGGC GCC^GGC
1099 GCCGGC
NgoMIV 990 GCCGGC G^CCGGC
1099 GCCGGC
PleI 112 GACTC GAGTC(4/5)
400 GACTC
SbfI 947 CCTGCAGG CCTGCA^GG
1143 CCTGCAGG
SfaNI 582 GATGC GCATC(5/9)
824 GCATC
Sse8387I 947 CCTGCAGG CCTGCA^GG
1143 CCTGCAGG
TspRI 51 CAGTG CA[GC]TG[ACGT][ACGT]^
886 CAGTG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI BanI BbeI BbvI BcnI
BglI BmyI BsaHI BsaJI BseRI
BsiEI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
ItaI KasI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PstI RsaI SacII Sau3AI Sau96I
ScrFI SfcI SfoI SmaI TseI
XmaCI XmaI