Restriction map for
pFN21AE6018
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 571 GTAGAC GT^[AC][GT]AC
AcsI 495 GAATTT [GA]^AATT[CT]
AocI 780 CCTGAGG CC^T[ACGT]AGG
ApaI 800 GGGCCC GGGCC^C
ApoI 495 GAATTT [GA]^AATT[CT]
Asp700I 525 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 441 GAGCTC G[AT]GC[AT]^C
AspI 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 787 CCTAGG C^CTAGG
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BcnI 798 CCGGG CC^[GC]GG
BlnI 787 CCTAGG C^CTAGG
BpmI 427 CTCCAG CTGGAG(16/14)
BsiHKAI 441 GAGCTC G[AT]GC[AT]^C
BsmBI 4 GAGACG CGTCTC(1/5)
BspDI 463 ATCGAT AT^CGAT
BspHI 407 TCATGA T^CATGA
BspMI 721 ACCTGC ACCTGC(4/8)
BstYI 328 GGATCT [GA]^GATC[CT]
Bsu36I 780 CCTGAGG CC^T[ACGT]AGG
ClaI 463 ATCGAT AT^CGAT
DsaI 89 CCGTGG C^C[GA][CT]GG
Eco81I 780 CCTGAGG CC^T[ACGT]AGG
EcoT14I 787 CCTAGG C^C[AT][AT]GG
HapII 798 CCGG C^CGG
HpaII 798 CCGG C^CGG
MaeII 7 ACGT A^CGT
MaeIII 745 GTCAC ^GT[ACGT]AC
MflI 328 GGATCT [GA]^GATC[CT]
MspI 798 CCGG C^CGG
NciI 798 CCGGG CC^[GC]GG
PflFI 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 477 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 350 GACTC GAGTC(4/5)
PshAI 738 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 924 CTGCAG CTGCA^G
PvuII 740 CAGCTG CAG^CTG
RcaI 407 TCATGA T^CATGA
SacI 441 GAGCTC GAGCT^C
ScaI 64 AGTACT AGT^ACT
SfcI 924 CTGCAG C^T[GA][CT]AG
StyI 787 CCTAGG C^C[AT][AT]GG
TaqI 464 TCGA T^CGA
TfiI 411 GAATC G^A[AT]TC
Tsp45I 745 GTCAC ^GT[GC]AC
Tth111I 73 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 464 TCGA T^CGA
Van91I 477 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 275 CCATCTCCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 328 GGATCT [GA]^GATC[CT]
XmnI 525 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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BalI 374 TGGCCA TGG^CCA
716 TGGCCA
BbsI 103 GTCTTC GAAGAC(2/6)
613 GTCTTC
BfaI 667 CTAG C^TAG
788 CTAG
BpuAI 103 GTCTTC GAAGAC(2/6)
613 GTCTTC
BseRI 133 CTCCTC GAGGAG(10/8)
726 CTCCTC
BsmAI 4 GAGAC GTCTC(1/5)
425 GTCTC
BsmFI 348 GGGAC GGGAC(10/14)
670 GGGAC
BsrI 436 ACTGG ACTGG(1/-1)
18 CCAGT
BstF5I 402 CATCC GGATG(2/0)
578 CATCC
BstXI 429 CCAGATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
638 CCATCATCGTGG
DraII 710 AGGCCCT [GA]G^G[ACGT]CC[CT]
800 GGGCCCC
EaeI 374 TGGCCA [CT]^GGCC[GA]
716 TGGCCA
EcoO109I 710 AGGCCCT [GA]G^G[ACGT]CC[CT]
800 GGGCCCC
FokI 402 CATCC GGATG(9/13)
578 CATCC
HinfI 350 GACTC G^A[ACGT]TC
411 GAATC
MaeI 667 CTAG C^TAG
788 CTAG
MluNI 374 TGGCCA TGG^CCA
716 TGGCCA
MscI 374 TGGCCA TGG^CCA
716 TGGCCA
MspA1I 740 CAGCTG C[AC]G^C[GT]G
921 CCGCTG
SfaNI 543 GATGC GCATC(5/9)
50 GCATC
SmlI 513 CTTGAG C^T[CT][GA]AG
876 CTTGAG
StuI 698 AGGCCT AGG^CCT
784 AGGCCT
Tsp509I 303 AATT ^AATT
496 AATT
TspRI 110 CAGTG CA[GC]TG[ACGT][ACGT]^
902 CACTG
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# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI HaeIII HphI ItaI Ksp632I
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI TseI