##################################
# NO CUT ENZYMES (  192 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MfeI        MflI        MluI        MroI        
MseI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     Tth111I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

ApaI          293  GGGCCC               GGGCC^C
BalI          140  TGGCCA               TGG^CCA
BanI          154  GGCACC               G^G[CT][GA]CC
BpmI          106  CTGGAG               CTGGAG(16/14)
BstF5I        195  CATCC                GGATG(2/0)
BstXI          77  CCAAGCCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I           6  GCTCGC               GC[ACGT]^[ACGT]GC
CfoI          103  GCGC                 GCG^C
DsaI          257  CCGTGG               C^C[GA][CT]GG
EcoNI         210  CCTCATAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          165  CCCGC                CCCGC(4/6)
FokI          195  CATCC                GGATG(9/13)
HhaI          103  GCGC                 GCG^C
HinP1I        103  GCGC                 G^CGC
HinfI         109  GAGTC                G^A[ACGT]TC
MluNI         140  TGGCCA               TGG^CCA
MscI          140  TGGCCA               TGG^CCA
MslI          195  CATCCCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflMI         252  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          109  GAGTC                GAGTC(4/5)
PstI           42  CTGCAG               CTGCA^G
SfaNI         194  GCATC                GCATC(5/9)
SfcI           42  CTGCAG               C^T[GA][CT]AG
TspRI         302  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        252  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII         134  GGACC                G^G[AT]CC
              247  GGTCC               
BcnI          125  CCGGG                CC^[GC]GG
              131  CCGGG               
BlpI          180  GCTGAGC              GC^T[ACGT]AGC
              238  GCTGAGC             
Bpu1102I      180  GCTGAGC              GC^T[ACGT]AGC
              238  GCTGAGC             
BsmFI         133  GGGAC                GGGAC(10/14)
              248  GTCCC               
CelII         180  GCTGAGC              GC^T[ACGT]AGC
              238  GCTGAGC             
EaeI           74  CGGCCA               [CT]^GGCC[GA]
              140  TGGCCA              
HapII         125  CCGG                 C^CGG
              131  CCGG                
HpaII         125  CCGG                 C^CGG
              131  CCGG                
MspA1I         50  CCGCTG               C[AC]G^C[GT]G
              166  CCGCTG              
MspI          125  CCGG                 C^CGG
              131  CCGG                
NciI          125  CCGGG                CC^[GC]GG
              131  CCGGG               
PpuMI         133  GGGACCC              [GA]G^G[AT]CC[CT]
              246  GGGTCCC             


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BglI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cfr13I      DdeI        DraII       
EcoO109I    EcoRII      Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        MwoI        NlaIV       Sau96I      
ScrFI       SfiI        TseI