Restriction map for
pFN21AE4092
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BcgI BciVI BclI BfaI
BglI BlnI BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrDI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI XbaI XcmI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 406 GACGTC GACGT^C
AccI 384 GTCGAC GT^[AC][GT]AC
AccIII 308 TCCGGA T^CCGGA
AfaI 1275 GTAC GT^AC
AflII 400 CTTAAG C^TTAAG
AflIII 1164 ACACGT A^C[GA][CT]GT
AlwI 138 GATCC GGATC(4/5)
AocI 661 CCTGAGG CC^T[ACGT]AGG
ApaI 824 GGGCCC GGGCC^C
Asp700I 756 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 628 CACGTG CAC^GTG
BfrI 400 CTTAAG C^TTAAG
BlpI 201 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 201 GCTGAGC GC^T[ACGT]AGC
BsaAI 628 CACGTG [CT]AC^GT[GA]
BsaI 355 GAGACC GGTCTC(1/5)
BseAI 308 TCCGGA T^CCGGA
BsgI 475 CTGCAC GTGCAG(16/14)
Bsp1407I 1274 TGTACA T^GTACA
BspEI 308 TCCGGA T^CCGGA
BspMI 1200 GCAGGT ACCTGC(4/8)
BsrFI 285 GCCGGT [GA]^CCGG[CT]
BsrGI 1274 TGTACA T^GTACA
BstAPI 144 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 30 GGTCACC G^GT[ACGT]ACC
BstPI 30 GGTCACC G^GT[ACGT]ACC
Bsu36I 661 CCTGAGG CC^T[ACGT]AGG
CelII 201 GCTGAGC GC^T[ACGT]AGC
Cfr10I 285 GCCGGT [GA]^CCGG[CT]
DraIII 145 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 661 CCTGAGG CC^T[ACGT]AGG
EcoO65I 30 GGTCACC G^GT[ACGT]ACC
EcoT22I 454 ATGCAT ATGCA^T
HaeII 108 AGCGCC [GA]GCGC^[CT]
HinfI 382 GAGTC G^A[ACGT]TC
MroI 308 TCCGGA T^CCGGA
MseI 401 TTAA T^TAA
NsiI 454 ATGCAT ATGCA^T
NspI 800 GCATGC [GA]CATG^[CT]
PaeR7I 982 CTCGAG C^TCGAG
PflFI 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 382 GAGTC GAGTC(4/5)
PmaCI 628 CACGTG CAC^GTG
PmlI 628 CACGTG CAC^GTG
PpuMI 692 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 1275 GTAC GT^AC
SalI 384 GTCGAC G^TCGAC
SphI 800 GCATGC GCATG^C
SspBI 1274 TGTACA T^GTACA
StuI 677 AGGCCT AGG^CCT
Tru9I 401 TTAA T^TAA
Tth111I 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 982 CTCGAG C^TCGAG
XmnI 756 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 859 CGCG CG^CG
1036 CGCG
AcsI 1008 GAATTT [GA]^AATT[CT]
1180 AAATTC
AcyI 406 GACGTC G[GA]^CG[CT]C
1252 GACGCC
ApoI 1008 GAATTT [GA]^AATT[CT]
1180 AAATTC
BbsI 942 GAAGAC GAAGAC(2/6)
1392 GAAGAC
BbvCI 45 CCTCAGC CCTCAGC(-5/-2)
116 CCTCAGC
BglII 794 AGATCT A^GATCT
1375 AGATCT
BpuAI 942 GAAGAC GAAGAC(2/6)
1392 GAAGAC
BsaHI 406 GACGTC G[GA]^CG[CT]C
1252 GACGCC
BsaWI 308 TCCGGA [AT]^CCGG[AT]
443 ACCGGA
BseRI 379 GAGGAG GAGGAG(10/8)
664 GAGGAG
BsmAI 355 GAGAC GTCTC(1/5)
928 GTCTC
BsmFI 368 GGGAC GGGAC(10/14)
1238 GTCCC
BsrBI 1050 CCGCTC CCGCTC(-3/-3)
640 GAGCGG
BsrI 609 ACTGG ACTGG(1/-1)
636 ACTGG
BstUI 859 CGCG CG^CG
1036 CGCG
BstXI 734 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCAAGCAGGTGG
BstYI 794 AGATCT [GA]^GATC[CT]
1375 AGATCT
DraII 692 AGGACCC [GA]G^G[ACGT]CC[CT]
823 AGGGCCC
EarI 214 CTCTTC CTCTTC(1/4)
447 GAAGAG
EcoNI 359 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1128 CCTGAATGAGG
EcoO109I 692 AGGACCC [GA]G^G[ACGT]CC[CT]
823 AGGGCCC
EcoT14I 364 CCAAGG C^C[AT][AT]GG
582 CCTTGG
FauI 857 CCCGC CCCGC(4/6)
1037 GCGGG
HgaI 9 GACGC GACGC(5/10)
1252 GACGC
Hin1I 406 GACGTC G[GA]^CG[CT]C
1252 GACGCC
HincII 384 GTCGAC GT[CT]^[GA]AC
1168 GTCAAC
HindII 384 GTCGAC GT[CT]^[GA]AC
1168 GTCAAC
Ksp632I 214 CTCTTC CTCTTC(1/4)
447 GAAGAG
MflI 794 AGATCT [GA]^GATC[CT]
1375 AGATCT
MslI 846 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
912 CATGAACATG
MvnI 859 CGCG CG^CG
1036 CGCG
PflMI 141 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
480 CCAGAAGCTGG
PstI 331 CTGCAG CTGCA^G
591 CTGCAG
SexAI 146 ACCTGGT A^CC[AT]GGT
779 ACCTGGT
SmlI 400 CTTAAG C^T[CT][GA]AG
982 CTCGAG
StyI 364 CCAAGG C^C[AT][AT]GG
582 CCTTGG
Tsp509I 1009 AATT ^AATT
1181 AATT
Van91I 141 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
480 CCAGAAGCTGG
XhoII 794 AGATCT [GA]^GATC[CT]
1375 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI