Restriction map for
pFN21AE0953
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BciVI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaHI
BseAI BsgI BsiEI BsiWI BsiYI
BslI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI FseI FspI HaeII Hin1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 1014 GTCTAC GT^[AC][GT]AC
AvaI 1036 CCCGAG C^[CT]CG[GA]G
BanII 1031 GAGCTC G[GA]GC[CT]^C
BbsI 655 GTCTTC GAAGAC(2/6)
BcgI 292 CGAATCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1019 CCCGG CC^[GC]GG
BpuAI 655 GTCTTC GAAGAC(2/6)
BsaBI 991 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1061 GGTCTC GGTCTC(1/5)
BsaWI 206 ACCGGA [AT]^CCGG[AT]
BsoBI 1036 CCCGAG C^[CT]CG[GA]G
BstAPI 1085 GCATAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 433 GGTCACC G^GT[ACGT]ACC
BstPI 433 GGTCACC G^GT[ACGT]ACC
Cac8I 548 GCATGC GC[ACGT]^[ACGT]GC
CfoI 348 GCGC GCG^C
DraI 145 TTTAAA TTT^AAA
DrdI 892 GACTATGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 433 GGTCACC G^GT[ACGT]ACC
EcoRV 952 GATATC GAT^ATC
EcoT22I 550 ATGCAT ATGCA^T
FauI 284 GCGGG CCCGC(4/6)
HaeIII 583 GGCC GG^CC
HgaI 1286 GACGC GACGC(5/10)
HhaI 348 GCGC GCG^C
HinP1I 348 GCGC G^CGC
MamI 991 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 694 CATGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1019 CCCGG CC^[GC]GG
NsiI 550 ATGCAT ATGCA^T
PleI 1134 GACTC GAGTC(4/5)
PstI 300 CTGCAG CTGCA^G
SacI 1031 GAGCTC GAGCT^C
SapI 384 GAAGAGC GCTCTTC(1/4)
SexAI 1349 ACCTGGT A^CC[AT]GGT
SphI 548 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 822 AAATTT [GA]^AATT[CT]
1306 GAATTT
ApoI 822 AAATTT [GA]^AATT[CT]
1306 GAATTT
BclI 531 TGATCA T^GATCA
687 TGATCA
BseRI 90 GAGGAG GAGGAG(10/8)
427 GAGGAG
BsmBI 455 GAGACG CGTCTC(1/5)
1284 GAGACG
BsmI 6 GCATTC GAATGC(1/-1)
1094 GCATTC
BspHI 1137 TCATGA T^CATGA
1320 TCATGA
BsrDI 1406 CATTGC GCAATG(2/0)
1159 GCAATG
BstBI 381 TTCGAA TT^CGAA
1355 TTCGAA
DraII 178 AGGTCCT [GA]G^G[ACGT]CC[CT]
1179 AGGACCC
EarI 87 GAAGAG CTCTTC(1/4)
384 GAAGAG
EcoO109I 178 AGGTCCT [GA]G^G[ACGT]CC[CT]
1179 AGGACCC
EcoT14I 1113 CCTTGG C^C[AT][AT]GG
1394 CCTTGG
FbaI 531 TGATCA T^GATCA
687 TGATCA
HapII 207 CCGG C^CGG
1020 CCGG
HincII 217 GTTGAC GT[CT]^[GA]AC
964 GTTGAC
HindII 217 GTTGAC GT[CT]^[GA]AC
964 GTTGAC
HpaII 207 CCGG C^CGG
1020 CCGG
Ksp632I 87 GAAGAG CTCTTC(1/4)
384 GAAGAG
MspI 207 CCGG C^CGG
1020 CCGG
NspI 548 GCATGC [GA]CATG^[CT]
552 GCATGT
NspV 381 TTCGAA TT^CGAA
1355 TTCGAA
PpuMI 178 AGGTCCT [GA]G^G[AT]CC[CT]
1179 AGGACCC
RcaI 1137 TCATGA T^CATGA
1320 TCATGA
SfaNI 672 GATGC GCATC(5/9)
1413 GCATC
SfcI 300 CTGCAG C^T[GA][CT]AG
1026 CTACAG
SfuI 381 TTCGAA TT^CGAA
1355 TTCGAA
StyI 1113 CCTTGG C^C[AT][AT]GG
1394 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BfaI BglII BmyI
BsaJI BsiHKAI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScaI ScrFI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII