Restriction map for
pFN21AE5452
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI AlwI
AocI Aor51HI ApoI AscI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AgeI 260 ACCGGT A^CCGGT
Alw44I 172 GTGCAC G^TGCAC
ApaI 25 GGGCCC GGGCC^C
ApaLI 172 GTGCAC G^TGCAC
AseI 433 ATTAAT AT^TAAT
AsnI 433 ATTAAT AT^TAAT
AvaI 213 CTCGGG C^[CT]CG[GA]G
BanI 944 GGCACC G^G[CT][GA]CC
BanII 25 GGGCCC G[GA]GC[CT]^C
BbsI 241 GTCTTC GAAGAC(2/6)
BbvCI 624 CCTCAGC CCTCAGC(-5/-2)
BfaI 208 CTAG C^TAG
BglI 41 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 241 GTCTTC GAAGAC(2/6)
BsaAI 100 TACGTG [CT]AC^GT[GA]
BsaWI 260 ACCGGT [AT]^CCGG[AT]
BsiYI 207 CCTAGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 207 CCTAGCCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 213 CTCGGG C^[CT]CG[GA]G
BsrBI 45 GAGCGG CCGCTC(-3/-3)
BsrFI 260 ACCGGT [GA]^CCGG[CT]
BstEII 492 GGTGACC G^GT[ACGT]ACC
BstPI 492 GGTGACC G^GT[ACGT]ACC
Cfr10I 260 ACCGGT [GA]^CCGG[CT]
DpnII 859 GATC ^GATC
DraIII 175 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 259 GACCGGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 233 CGGCCA [CT]^GGCC[GA]
EarI 532 GAAGAG CTCTTC(1/4)
EcoO65I 492 GGTGACC G^GT[ACGT]ACC
EcoT22I 399 ATGCAT ATGCA^T
FauI 10 CCCGC CCCGC(4/6)
HaeII 163 AGCGCC [GA]GCGC^[CT]
HincII 553 GTCAAC GT[CT]^[GA]AC
HindII 553 GTCAAC GT[CT]^[GA]AC
HphI 492 GGTGA GGTGA(8/7)
Ksp632I 532 GAAGAG CTCTTC(1/4)
MaeI 208 CTAG C^TAG
MaeII 101 ACGT A^CGT
MboI 859 GATC ^GATC
MseI 434 TTAA T^TAA
NdeII 859 GATC ^GATC
NsiI 399 ATGCAT ATGCA^T
PinAI 260 ACCGGT A^CCGGT
PpuMI 217 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 196 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 433 ATTAAT AT^TAAT
PstI 14 CTGCAG CTGCA^G
SapI 532 GAAGAGC GCTCTTC(1/4)
Sau3AI 859 GATC ^GATC
ScaI 710 AGTACT AGT^ACT
SfiI 40 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 955 AGGCCT AGG^CCT
TfiI 1048 GATTC G^A[AT]TC
Tru9I 434 TTAA T^TAA
Tsp45I 493 GTGAC ^GT[GC]AC
Tsp509I 280 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AfaI 711 GTAC GT^AC
910 GTAC
AlwNI 115 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
744 CAGCCTCTG
AspHI 72 GTGCTC G[AT]GC[AT]^C
172 GTGCAC
BsgI 649 GTGCAG GTGCAG(16/14)
728 GTGCAG
BsiHKAI 72 GTGCTC G[AT]GC[AT]^C
172 GTGCAC
BsmAI 886 GAGAC GTCTC(1/5)
921 GTCTC
MaeIII 346 GTTAC ^GT[ACGT]AC
493 GTGAC
MspA1I 11 CCGCTG C[AC]G^C[GT]G
84 CAGCGG
NcoI 20 CCATGG C^CATGG
701 CCATGG
PleI 447 GAGTC GAGTC(4/5)
919 GAGTC
RsaI 711 GTAC GT^AC
910 GTAC
SfcI 14 CTGCAG C^T[GA][CT]AG
470 CTGTAG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsmFI Bsp1286I BsrDI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII ItaI MboII MnlI
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV Sau96I ScrFI SfaNI
StyI TaqI TseI TspRI TthHB8I