Restriction map for
pFN21AB7036
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstYI Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MaeII MflI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 541 GTATAC GT^[AC][GT]AC
AccII 756 CGCG CG^CG
AgeI 344 ACCGGT A^CCGGT
AspHI 685 GAGCAC G[AT]GC[AT]^C
AvaI 626 CCCGGG C^[CT]CG[GA]G
BalI 920 TGGCCA TGG^CCA
BbsI 265 GTCTTC GAAGAC(2/6)
BcgI 104 GCAATGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 685 GAGCAC G[AGT]GC[ACT]^C
BpmI 1024 CTCCAG CTGGAG(16/14)
BpuAI 265 GTCTTC GAAGAC(2/6)
BsaBI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 487 GAGACC GGTCTC(1/5)
BsaWI 344 ACCGGT [AT]^CCGG[AT]
BseRI 196 CTCCTC GAGGAG(10/8)
BsgI 303 CTGCAC GTGCAG(16/14)
BsiEI 342 CGACCG CG[GA][CT]^CG
BsiHKAI 685 GAGCAC G[AT]GC[AT]^C
BsmFI 1039 GGGAC GGGAC(10/14)
BsmI 1096 GAATGC GAATGC(1/-1)
BsoBI 626 CCCGGG C^[CT]CG[GA]G
Bsp1286I 685 GAGCAC G[AGT]GC[ACT]^C
BspHI 329 TCATGA T^CATGA
BspMI 191 ACCTGC ACCTGC(4/8)
BsrDI 104 GCAATG GCAATG(2/0)
Bst1107I 541 GTATAC GTA^TAC
BstUI 756 CGCG CG^CG
BstZ17I 541 GTATAC GTA^TAC
FauI 579 CCCGC CCCGC(4/6)
HinfI 37 GACTC G^A[ACGT]TC
MamI 54 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 159 CAATTG C^AATTG
MluNI 920 TGGCCA TGG^CCA
MscI 920 TGGCCA TGG^CCA
MslI 60 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 159 CAATTG C^AATTG
MvnI 756 CGCG CG^CG
NaeI 140 GCCGGC GCC^GGC
NcoI 1078 CCATGG C^CATGG
NgoMIV 140 GCCGGC G^CCGGC
PinAI 344 ACCGGT A^CCGGT
PleI 37 GACTC GAGTC(4/5)
PpuMI 976 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1041 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 912 CTGCAG CTGCA^G
RcaI 329 TCATGA T^CATGA
SapI 903 GCTCTTC GCTCTTC(1/4)
SfcI 912 CTGCAG C^T[GA][CT]AG
SmaI 626 CCCGGG CCC^GGG
SphI 46 GCATGC GCATG^C
TaqI 341 TCGA T^CGA
TthHB8I 341 TCGA T^CGA
XmaCI 626 CCCGGG C^CCGGG
XmaI 626 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Acc65I 374 GGTACC G^GTACC
893 GGTACC
AlwI 630 GGATC GGATC(4/5)
644 GGATC
Asp718I 374 GGTACC G^GTACC
893 GGTACC
BclI 332 TGATCA T^GATCA
587 TGATCA
BsmAI 487 GAGAC GTCTC(1/5)
878 GAGAC
BsrFI 140 GCCGGC [GA]^CCGG[CT]
344 ACCGGT
BsrI 705 ACTGG ACTGG(1/-1)
394 CCAGT
BstEII 387 GGTCACC G^GT[ACGT]ACC
564 GGTCACC
BstPI 387 GGTCACC G^GT[ACGT]ACC
564 GGTCACC
CfoI 353 GCGC GCG^C
868 GCGC
Cfr10I 140 GCCGGC [GA]^CCGG[CT]
344 ACCGGT
DraII 721 GGGGCCT [GA]G^G[ACGT]CC[CT]
976 AGGACCC
EcoO109I 721 GGGGCCT [GA]G^G[ACGT]CC[CT]
976 AGGACCC
EcoO65I 387 GGTCACC G^GT[ACGT]ACC
564 GGTCACC
EcoT14I 176 CCTTGG C^C[AT][AT]GG
1078 CCATGG
FbaI 332 TGATCA T^GATCA
587 TGATCA
HhaI 353 GCGC GCG^C
868 GCGC
HinP1I 353 GCGC G^CGC
868 GCGC
KpnI 374 GGTACC GGTAC^C
893 GGTACC
MseI 320 TTAA T^TAA
421 TTAA
MspA1I 235 CAGCTG C[AC]G^C[GT]G
967 CAGCTG
NspI 46 GCATGC [GA]CATG^[CT]
1177 ACATGC
PvuII 235 CAGCTG CAG^CTG
967 CAGCTG
StyI 176 CCTTGG C^C[AT][AT]GG
1078 CCATGG
Tru9I 320 TTAA T^TAA
421 TTAA
XcmI 695 CCACAGTGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1026 CCAGGTGTGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BcnI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TseI Tsp45I Tsp509I TspRI