##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstNI       BstUI       BstXI       BstYI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DpnII       DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboI        
MboII       MflI        MluI        MluNI       MroI        
MscI        MseI        MspA1I      MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          196  CCGC                 CCGC(-3/-1)
AocI           85  CCTAAGG              CC^T[ACGT]AGG
BanI           69  GGTGCC               G^G[CT][GA]CC
BbvI          140  GCTGC                GCAGC(8/12)
BcnI           99  CCGGG                CC^[GC]GG
BssKI          99  CCGGG                ^CC[ACGT]GG
BstEII        123  GGTGACC              G^GT[ACGT]ACC
BstF5I         81  CATCC                GGATG(2/0)
BstPI         123  GGTGACC              G^GT[ACGT]ACC
Bsu36I         85  CCTAAGG              CC^T[ACGT]AGG
DdeI           86  CTAAG                C^T[ACGT]AG
DraII          90  GGGTCCT              [GA]G^G[ACGT]CC[CT]
Eco81I         85  CCTAAGG              CC^T[ACGT]AGG
EcoO109I       90  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       123  GGTGACC              G^GT[ACGT]ACC
EcoT14I        73  CCTTGG               C^C[AT][AT]GG
Fnu4HI        140  GCTGC                GC^[ACGT]GC
FokI           81  CATCC                GGATG(9/13)
HapII          99  CCGG                 C^CGG
HpaII          99  CCGG                 C^CGG
HphI          123  GGTGA                GGTGA(8/7)
ItaI          140  GCTGC                GC^[ACGT]GC
MaeIII        124  GTGAC                ^GT[ACGT]AC
MfeI          199  CAATTG               C^AATTG
MspI           99  CCGG                 C^CGG
MunI          199  CAATTG               C^AATTG
NciI           99  CCGGG                CC^[GC]GG
NlaIII        117  CATG                 CATG^
PpuMI          90  GGGTCCT              [GA]G^G[AT]CC[CT]
ScrFI          99  CCGGG                CC^[ACGT]GG
StyI           73  CCTTGG               C^C[AT][AT]GG
TseI          140  GCTGC                G^C[AT]GC
Tsp45I        124  GTGAC                ^GT[GC]AC
TspRI          18  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AvaII          91  GGTCC                G^G[AT]CC
              193  GGACC               
BsaJI          73  CCTTGG               C^C[ACGT][ACGT]GG
               99  CCGGGG              
BsiYI          94  CCTAACCGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              184  CCTCTCTTTGG         
BslI           94  CCTAACCGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              184  CCTCTCTTTGG         
MwoI           53  GCTTCGTCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              163  GCAATTCTGGC         
NlaIV          69  GGTGCC               GG[ACGT]^[ACGT]CC
               90  GGGTCC              
Tsp509I       165  AATT                 ^AATT
              200  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

Cfr13I      HaeIII      MnlI        MslI        Sau96I