Restriction map for
pFN21AE9691
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstXI BstZ17I Bsu36I
CelII CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FokI
FseI FspI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
MluNI MroI MscI MslI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI Van91I
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 445 CGCG CG^CG
AflII 1632 CTTAAG C^TTAAG
AflIII 459 ACGTGT A^C[GA][CT]GT
AspI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1417 CTCGGG C^[CT]CG[GA]G
AvaII 720 GGACC G^G[AT]CC
BamHI 744 GGATCC G^GATCC
BanII 1205 GGGCTC G[GA]GC[CT]^C
BbvCI 1624 GCTGAGG CCTCAGC(-5/-2)
BfrI 1632 CTTAAG C^TTAAG
BglII 702 AGATCT A^GATCT
BsiYI 303 CCATGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 303 CCATGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 1417 CTCGGG C^[CT]CG[GA]G
BspDI 1433 ATCGAT AT^CGAT
BsrDI 799 GCAATG GCAATG(2/0)
BsrFI 617 ACCGGC [GA]^CCGG[CT]
BssSI 686 CTCGTG CACGAG(-5/-1)
BstUI 445 CGCG CG^CG
Cfr10I 617 ACCGGC [GA]^CCGG[CT]
Cfr13I 720 GGACC G^G[ACGT]CC
ClaI 1433 ATCGAT AT^CGAT
EcoT22I 1394 ATGCAT ATGCA^T
HaeIII 1629 GGCC GG^CC
HapII 618 CCGG C^CGG
HgaI 1621 GACGC GACGC(5/10)
HindIII 1687 AAGCTT A^AGCTT
HpaII 618 CCGG C^CGG
MfeI 118 CAATTG C^AATTG
MspI 618 CCGG C^CGG
MunI 118 CAATTG C^AATTG
MvnI 445 CGCG CG^CG
NcoI 303 CCATGG C^CATGG
NlaIV 744 GGATCC GG[ACGT]^[ACGT]CC
NsiI 1394 ATGCAT ATGCA^T
PflFI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 1066 GTCGAC G^TCGAC
Sau96I 720 GGACC G^G[ACGT]CC
SfcI 910 CTACAG C^T[GA][CT]AG
SpeI 87 ACTAGT A^CTAGT
StuI 1628 AGGCCT AGG^CCT
Tsp45I 840 GTCAC ^GT[GC]AC
Tth111I 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1209 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 613 GTAGAC GT^[AC][GT]AC
1066 GTCGAC
AlwI 745 GATCC GGATC(4/5)
744 GGATC
AspHI 1363 GTGCTC G[AT]GC[AT]^C
1412 GTGCTC
BsaI 1795 GAGACC GGTCTC(1/5)
923 GGTCTC
BsgI 68 CTGCAC GTGCAG(16/14)
463 GTGCAG
BsiHKAI 1363 GTGCTC G[AT]GC[AT]^C
1412 GTGCTC
BsrI 1528 CCAGT ACTGG(1/-1)
1800 CCAGT
BstYI 702 AGATCT [GA]^GATC[CT]
744 GGATCC
CfoI 444 GCGC GCG^C
606 GCGC
DsaI 303 CCATGG C^C[GA][CT]GG
1562 CCGTGG
EcoT14I 303 CCATGG C^C[AT][AT]GG
740 CCTTGG
FauI 1615 CCCGC CCCGC(4/6)
1203 GCGGG
HhaI 444 GCGC GCG^C
606 GCGC
HinP1I 444 GCGC G^CGC
606 GCGC
HincII 1066 GTCGAC GT[CT]^[GA]AC
1402 GTCAAC
HindII 1066 GTCGAC GT[CT]^[GA]AC
1402 GTCAAC
MaeIII 840 GTCAC ^GT[ACGT]AC
891 GTTAC
MflI 702 AGATCT [GA]^GATC[CT]
744 GGATCC
NspI 473 GCATGT [GA]CATG^[CT]
1396 GCATGT
PvuII 385 CAGCTG CAG^CTG
1573 CAGCTG
SapI 52 GAAGAGC GCTCTTC(1/4)
493 GCTCTTC
SmlI 202 CTCAAG C^T[CT][GA]AG
1632 CTTAAG
StyI 303 CCATGG C^C[AT][AT]GG
740 CCTTGG
TspRI 1410 CAGTG CA[GC]TG[ACGT][ACGT]^
1801 CAGTG
XhoII 702 AGATCT [GA]^GATC[CT]
744 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsmAI Bsp1286I
BssKI BstNI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI HinfI HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp509I TthHB8I