Restriction map for
pFN21AE2066
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BbrPI BbvCI BcgI BclI
BcnI BglI BlnI BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRV EcoT22I FauI
FbaI FseI FspI HapII HpaII
KpnI KspI MamI MluI MluNI
MroI MscI MspI MvnI NaeI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Alw44I 50 GTGCAC G^TGCAC
AlwNI 319 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 10 CCTAAGG CC^T[ACGT]AGG
ApaLI 50 GTGCAC G^TGCAC
AvaII 100 GGACC G^G[AT]CC
BamHI 314 GGATCC G^GATCC
BbeI 15 GGCGCC GGCGC^C
BciVI 106 GTATCC GTATCC(6/5)
BlpI 559 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 559 GCTAAGC GC^T[ACGT]AGC
BsmBI 1741 CGTCTC CGTCTC(1/5)
BspHI 2079 TCATGA T^CATGA
BspMI 588 GCAGGT ACCTGC(4/8)
BstAPI 394 GCAAATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 10 CCTAAGG CC^T[ACGT]AGG
CelII 559 GCTAAGC GC^T[ACGT]AGC
CfoI 16 GCGC GCG^C
Cfr13I 100 GGACC G^G[ACGT]CC
DraI 130 TTTAAA TTT^AAA
EaeI 1454 CGGCCA [CT]^GGCC[GA]
Eco81I 10 CCTAAGG CC^T[ACGT]AGG
EcoRI 494 GAATTC G^AATTC
EcoT14I 1457 CCAAGG C^C[AT][AT]GG
HaeII 15 GGCGCC [GA]GCGC^[CT]
HgaI 1740 GCGTC GACGC(5/10)
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
HpaI 1395 GTTAAC GTT^AAC
HphI 593 TCACC GGTGA(8/7)
KasI 15 GGCGCC G^GCGCC
MaeII 294 ACGT A^CGT
MfeI 1204 CAATTG C^AATTG
MslI 573 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1204 CAATTG C^AATTG
NarI 15 GGCGCC GG^CGCC
NdeI 1874 CATATG CA^TATG
NspI 1920 GCATGT [GA]CATG^[CT]
PflMI 318 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2079 TCATGA T^CATGA
Sau96I 100 GGACC G^G[ACGT]CC
SfoI 15 GGCGCC GGC^GCC
SspI 835 AATATT AAT^ATT
StyI 1457 CCAAGG C^C[AT][AT]GG
Tsp45I 592 GTCAC ^GT[GC]AC
Van91I 318 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 15 GGCGCC G[GA]^CG[CT]C
1739 GGCGTC
AflII 814 CTTAAG C^TTAAG
1420 CTTAAG
Asp700I 24 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1000 GAAAAGCTTC
AspI 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1551 GACCAAGTC
BanI 15 GGCGCC G^G[CT][GA]CC
298 GGTGCC
BanII 1509 GAGCTC G[GA]GC[CT]^C
2144 GAGCTC
BfrI 814 CTTAAG C^TTAAG
1420 CTTAAG
BsaHI 15 GGCGCC G[GA]^CG[CT]C
1739 GGCGTC
BseRI 1744 CTCCTC GAGGAG(10/8)
829 GAGGAG
BsgI 365 CTGCAC GTGCAG(16/14)
1838 GTGCAG
BstEII 475 GGTAACC G^GT[ACGT]ACC
591 GGTCACC
BstPI 475 GGTAACC G^GT[ACGT]ACC
591 GGTCACC
Cac8I 186 GCCTGC GC[ACGT]^[ACGT]GC
1986 GCTTGC
EarI 610 GAAGAG CTCTTC(1/4)
1210 GAAGAG
EcoO65I 475 GGTAACC G^GT[ACGT]ACC
591 GGTCACC
HaeIII 1311 GGCC GG^CC
1455 GGCC
Hin1I 15 GGCGCC G[GA]^CG[CT]C
1739 GGCGTC
HindIII 501 AAGCTT A^AGCTT
1003 AAGCTT
Ksp632I 610 GAAGAG CTCTTC(1/4)
1210 GAAGAG
PflFI 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1551 GACCAAGTC
PstI 586 CTGCAG CTGCA^G
1546 CTGCAG
PvuII 883 CAGCTG CAG^CTG
1663 CAGCTG
SacI 1509 GAGCTC GAGCT^C
2144 GAGCTC
Tth111I 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1551 GACCAAGTC
XbaI 1225 TCTAGA T^CTAGA
1539 TCTAGA
XmnI 24 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1000 GAAAAGCTTC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI DdeI
DpnII EcoRII Fnu4HI FokI HincII
HindII HinfI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII