Restriction map for
pFN21AB7037
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BbrPI BbsI BbvCI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstBI BstZ17I CelII CpoI
DraI DraII DraIII DrdI Eam1105I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I RsrII SacI SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp509I Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AocI 756 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1193 AGCGCT AGC^GCT
ApaI 1410 GGGCCC GGGCC^C
AscI 1300 GGCGCGCC GG^CGCGCC
AspHI 319 GTGCTC G[AT]GC[AT]^C
AviII 1079 TGCGCA TGC^GCA
BamHI 761 GGATCC G^GATCC
BcgI 890 GCATTGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 456 GTATCC GTATCC(6/5)
BseRI 618 CTCCTC GAGGAG(10/8)
BsgI 384 CTGCAC GTGCAG(16/14)
BsiHKAI 319 GTGCTC G[AT]GC[AT]^C
BsmFI 1456 GGGAC GGGAC(10/14)
BspDI 421 ATCGAT AT^CGAT
BspHI 722 TCATGA T^CATGA
BssSI 781 CACGAG CACGAG(-5/-1)
BstXI 134 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 756 CCTGAGG CC^T[ACGT]AGG
ClaI 421 ATCGAT AT^CGAT
Eco47III 1193 AGCGCT AGC^GCT
Eco81I 756 CCTGAGG CC^T[ACGT]AGG
FspI 1079 TGCGCA TGC^GCA
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
MseI 851 TTAA T^TAA
NaeI 558 GCCGGC GCC^GGC
NcoI 263 CCATGG C^CATGG
NgoMIV 558 GCCGGC G^CCGGC
NspI 1535 ACATGC [GA]CATG^[CT]
PaeR7I 93 CTCGAG C^TCGAG
PstI 451 CTGCAG CTGCA^G
PvuI 423 CGATCG CGAT^CG
RcaI 722 TCATGA T^CATGA
SapI 1416 GCTCTTC GCTCTTC(1/4)
SfaNI 1082 GCATC GCATC(5/9)
SfcI 451 CTGCAG C^T[GA][CT]AG
SmlI 93 CTCGAG C^T[CT][GA]AG
TfiI 3 GAATC G^A[AT]TC
Tru9I 851 TTAA T^TAA
XhoI 93 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 666 GTAC GT^AC
800 GTAC
AlwNI 35 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
501 CAGTGTCTG
AvaII 1256 GGACC G^G[AT]CC
1457 GGACC
BsrBI 1414 CCGCTC CCGCTC(-3/-3)
1242 GAGCGG
BssHII 1068 GCGCGC G^CGCGC
1301 GCGCGC
BstEII 468 GGTCACC G^GT[ACGT]ACC
588 GGTCACC
BstPI 468 GGTCACC G^GT[ACGT]ACC
588 GGTCACC
BstYI 344 GGATCT [GA]^GATC[CT]
761 GGATCC
EarI 613 CTCTTC CTCTTC(1/4)
1417 CTCTTC
EcoO65I 468 GGTCACC G^GT[ACGT]ACC
588 GGTCACC
Ksp632I 613 CTCTTC CTCTTC(1/4)
1417 CTCTTC
MaeII 883 ACGT A^CGT
1502 ACGT
MflI 344 GGATCT [GA]^GATC[CT]
761 GGATCC
MslI 135 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CATCGGGGTG
RsaI 666 GTAC GT^AC
800 GTAC
SfiI 1387 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1497 GGCCGACGTGGCC
XhoII 344 GGATCT [GA]^GATC[CT]
761 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsiEI BsiYI
BslI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EagI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NotI PleI PvuII SacII
Sau3AI Sau96I ScrFI SfoI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI