Restriction map for
pFN21AE2451
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaII AviII AvrII BalI BamHI
BanI BbeI BcgI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I Cac8I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MscI MunI MvnI MwoI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tsp509I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 723 GTCTAC GT^[AC][GT]AC
AccIII 615 TCCGGA T^CCGGA
AluI 492 AGCT AG^CT
AlwI 774 GGATC GGATC(4/5)
ApaI 661 GGGCCC GGGCC^C
AspHI 976 GTGCTC G[AT]GC[AT]^C
AspI 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 661 GGGCCC G[GA]GC[CT]^C
BbvCI 832 CCTCAGC CCTCAGC(-5/-2)
BclI 950 TGATCA T^GATCA
BcnI 102 CCGGG CC^[GC]GG
BfaI 1054 CTAG C^TAG
BsaBI 948 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 134 GGTCTC GGTCTC(1/5)
BsaJI 965 CCTTGG C^C[ACGT][ACGT]GG
BseAI 615 TCCGGA T^CCGGA
BseRI 830 CTCCTC GAGGAG(10/8)
BsiHKAI 976 GTGCTC G[AT]GC[AT]^C
BsmFI 262 GGGAC GGGAC(10/14)
BspEI 615 TCCGGA T^CCGGA
BsrDI 561 CATTGC GCAATG(2/0)
BstYI 774 GGATCT [GA]^GATC[CT]
DraII 660 AGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 100 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 979 CTCTTC CTCTTC(1/4)
EcoNI 612 CCTTCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 660 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 965 CCTTGG C^C[AT][AT]GG
FbaI 950 TGATCA T^GATCA
HaeII 198 GGCGCT [GA]GCGC^[CT]
Ksp632I 979 CTCTTC CTCTTC(1/4)
MaeI 1054 CTAG C^TAG
MamI 948 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 774 GGATCT [GA]^GATC[CT]
MroI 615 TCCGGA T^CCGGA
MseI 298 TTAA T^TAA
NciI 102 CCGGG CC^[GC]GG
NlaIV 661 GGGCCC GG[ACGT]^[ACGT]CC
NspI 152 GCATGT [GA]CATG^[CT]
PflFI 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 764 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 57 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 674 CTGCAG CTGCA^G
PvuII 491 CAGCTG CAG^CTG
SapI 978 GCTCTTC GCTCTTC(1/4)
SfaNI 844 GCATC GCATC(5/9)
SmlI 231 CTCAAG C^T[CT][GA]AG
StyI 965 CCTTGG C^C[AT][AT]GG
Tru9I 298 TTAA T^TAA
Tth111I 736 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 764 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 774 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 123 GTAC GT^AC
327 GTAC
AflIII 584 ACACGT A^C[GA][CT]GT
794 ACGTGT
AvaI 270 CCCGAG C^[CT]CG[GA]G
643 CCCGAG
BbrPI 585 CACGTG CAC^GTG
793 CACGTG
BsaAI 585 CACGTG [CT]AC^GT[GA]
793 CACGTG
BsgI 494 CTGCAC GTGCAG(16/14)
1093 GTGCAG
BsoBI 270 CCCGAG C^[CT]CG[GA]G
643 CCCGAG
BstEII 97 GGTCACC G^GT[ACGT]ACC
225 GGTGACC
BstPI 97 GGTCACC G^GT[ACGT]ACC
225 GGTGACC
CfoI 199 GCGC GCG^C
381 GCGC
Cfr13I 661 GGGCC G^G[ACGT]CC
662 GGCCC
EcoO65I 97 GGTCACC G^GT[ACGT]ACC
225 GGTGACC
HaeIII 662 GGCC GG^CC
1120 GGCC
HhaI 199 GCGC GCG^C
381 GCGC
HinP1I 199 GCGC G^CGC
381 GCGC
MslI 339 CATAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CATGTCAATG
MspA1I 318 CAGCGG C[AC]G^C[GT]G
491 CAGCTG
PmaCI 585 CACGTG CAC^GTG
793 CACGTG
PmlI 585 CACGTG CAC^GTG
793 CACGTG
RsaI 123 GTAC GT^AC
327 GTAC
Sau96I 661 GGGCC G^G[ACGT]CC
662 GGCCC
SfcI 391 CTACAG C^T[GA][CT]AG
674 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AlwNI BbsI BbvI BmyI
BpmI BpuAI BsaWI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspI MvaI NdeII NlaIII
PleI Sau3AI ScrFI TfiI TseI
Tsp45I TspRI