################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BseRI BsgI BsiEI BsiWI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI CpoI DrdI EagI EclXI Eco52I EcoNI EcoRI EcoRV FseI FspI HpaI KasI KpnI KspI MfeI MluI MspA1I MunI NarI NdeI NheI NotI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TaqI TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 73 enzymes) # ################################### AccIII 653 TCCGGA T^CCGGA AcyI 1330 GACGCC G[GA]^CG[CT]C AhdI 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1053 GTGCAC G^TGCAC AlwNI 1381 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 660 CCTCAGG CC^T[ACGT]AGG Aor51HI 329 AGCGCT AGC^GCT ApaI 258 GGGCCC GGGCC^C ApaLI 1053 GTGCAC G^TGCAC AspEI 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1053 GTGCAC G[AT]GC[AT]^C AspI 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1112 CCCGGG C^[CT]CG[GA]G BamHI 212 GGATCC G^GATCC BanI 1243 GGCACC G^G[CT][GA]CC BclI 1337 TGATCA T^GATCA BfaI 391 CTAG C^TAG BsaBI 1338 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1330 GACGCC G[GA]^CG[CT]C BsaWI 653 TCCGGA [AT]^CCGG[AT] BseAI 653 TCCGGA T^CCGGA BsiHKAI 1053 GTGCAC G[AT]GC[AT]^C BsmAI 571 GAGAC GTCTC(1/5) BsmBI 571 GAGACG CGTCTC(1/5) BsmI 870 GAATGC GAATGC(1/-1) BsoBI 1112 CCCGGG C^[CT]CG[GA]G Bsp1407I 491 TGTACA T^GTACA BspEI 653 TCCGGA T^CCGGA BsrBI 1270 CCGCTC CCGCTC(-3/-3) BsrGI 491 TGTACA T^GTACA BstAPI 1046 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1281 GGTCACC G^GT[ACGT]ACC BstPI 1281 GGTCACC G^GT[ACGT]ACC Bsu36I 660 CCTCAGG CC^T[ACGT]AGG CfoI 330 GCGC GCG^C DraI 516 TTTAAA TTT^AAA DraIII 184 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 593 CCATGG C^C[GA][CT]GG Eam1105I 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 799 CTCTTC CTCTTC(1/4) Eco47III 329 AGCGCT AGC^GCT Eco81I 660 CCTCAGG CC^T[ACGT]AGG EcoO65I 1281 GGTCACC G^GT[ACGT]ACC EcoT22I 382 ATGCAT ATGCA^T FbaI 1337 TGATCA T^GATCA HaeII 329 AGCGCT [GA]GCGC^[CT] HgaI 1330 GACGC GACGC(5/10) HhaI 330 GCGC GCG^C Hin1I 1330 GACGCC G[GA]^CG[CT]C HinP1I 330 GCGC G^CGC HincII 1119 GTCAAC GT[CT]^[GA]AC HindII 1119 GTCAAC GT[CT]^[GA]AC HindIII 1027 AAGCTT A^AGCTT Ksp632I 799 CTCTTC CTCTTC(1/4) MaeI 391 CTAG C^TAG MamI 1338 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 653 TCCGGA T^CCGGA NaeI 1042 GCCGGC GCC^GGC NcoI 593 CCATGG C^CATGG NgoMIV 1042 GCCGGC G^CCGGC NruI 601 TCGCGA TCG^CGA NsiI 382 ATGCAT ATGCA^T PflFI 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ScaI 16 AGTACT AGT^ACT SmaI 1112 CCCGGG CCC^GGG SmlI 1399 CTCAAG C^T[CT][GA]AG SphI 701 GCATGC GCATG^C SspBI 491 TGTACA T^GTACA StuI 173 AGGCCT AGG^CCT Tth111I 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 890 CCATCCTGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1112 CCCGGG C^CCGGG XmaI 1112 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 243 CGCG CG^CG 602 CGCG BalI 689 TGGCCA TGG^CCA 1187 TGGCCA BbvI 639 GCTGC GCAGC(8/12) 1395 GCTGC BcnI 1112 CCCGG CC^[GC]GG 1113 CCGGG BpmI 415 CTCCAG CTGGAG(16/14) 1276 CTGGAG BspMI 953 ACCTGC ACCTGC(4/8) 995 ACCTGC BsrDI 288 CATTGC GCAATG(2/0) 1038 CATTGC BsrFI 761 GCCGGT [GA]^CCGG[CT] 1042 GCCGGC BstUI 243 CGCG CG^CG 602 CGCG BstXI 44 CCATCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 80 CCAAGAAGTTGG Cfr10I 761 GCCGGT [GA]^CCGG[CT] 1042 GCCGGC EaeI 689 TGGCCA [CT]^GGCC[GA] 1187 TGGCCA EcoT14I 152 CCAAGG C^C[AT][AT]GG 593 CCATGG MaeII 1076 ACGT A^CGT 1288 ACGT MluNI 689 TGGCCA TGG^CCA 1187 TGGCCA MscI 689 TGGCCA TGG^CCA 1187 TGGCCA MvnI 243 CGCG CG^CG 602 CGCG NciI 1112 CCCGG CC^[GC]GG 1113 CCGGG PflMI 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1380 CCAGGCCCTGG PpuMI 24 GGGTCCC [GA]G^G[AT]CC[CT] 1372 GGGACCT PstI 1066 CTGCAG CTGCA^G 1082 CTGCAG SexAI 185 ACCTGGT A^CC[AT]GGT 1211 ACCTGGT SfcI 1066 CTGCAG C^T[GA][CT]AG 1082 CTGCAG StyI 152 CCAAGG C^C[AT][AT]GG 593 CCATGG TseI 639 GCTGC G^C[AT]GC 1395 GCTGC Tsp509I 50 AATT ^AATT 514 AATT Van91I 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1380 CCAGGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanII BbsI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I Tsp45I TspRI XhoII