Restriction map for
pFN21AE6478
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# NO CUT ENZYMES ( 98 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseRI
BsgI BsiEI BsiWI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
EagI EclXI Eco52I EcoNI EcoRI
EcoRV FseI FspI HpaI KasI
KpnI KspI MfeI MluI MspA1I
MunI NarI NdeI NheI NotI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI TaqI TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AccIII 653 TCCGGA T^CCGGA
AcyI 1330 GACGCC G[GA]^CG[CT]C
AhdI 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1053 GTGCAC G^TGCAC
AlwNI 1381 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 660 CCTCAGG CC^T[ACGT]AGG
Aor51HI 329 AGCGCT AGC^GCT
ApaI 258 GGGCCC GGGCC^C
ApaLI 1053 GTGCAC G^TGCAC
AspEI 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1053 GTGCAC G[AT]GC[AT]^C
AspI 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1112 CCCGGG C^[CT]CG[GA]G
BamHI 212 GGATCC G^GATCC
BanI 1243 GGCACC G^G[CT][GA]CC
BclI 1337 TGATCA T^GATCA
BfaI 391 CTAG C^TAG
BsaBI 1338 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1330 GACGCC G[GA]^CG[CT]C
BsaWI 653 TCCGGA [AT]^CCGG[AT]
BseAI 653 TCCGGA T^CCGGA
BsiHKAI 1053 GTGCAC G[AT]GC[AT]^C
BsmAI 571 GAGAC GTCTC(1/5)
BsmBI 571 GAGACG CGTCTC(1/5)
BsmI 870 GAATGC GAATGC(1/-1)
BsoBI 1112 CCCGGG C^[CT]CG[GA]G
Bsp1407I 491 TGTACA T^GTACA
BspEI 653 TCCGGA T^CCGGA
BsrBI 1270 CCGCTC CCGCTC(-3/-3)
BsrGI 491 TGTACA T^GTACA
BstAPI 1046 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1281 GGTCACC G^GT[ACGT]ACC
BstPI 1281 GGTCACC G^GT[ACGT]ACC
Bsu36I 660 CCTCAGG CC^T[ACGT]AGG
CfoI 330 GCGC GCG^C
DraI 516 TTTAAA TTT^AAA
DraIII 184 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 593 CCATGG C^C[GA][CT]GG
Eam1105I 55 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 799 CTCTTC CTCTTC(1/4)
Eco47III 329 AGCGCT AGC^GCT
Eco81I 660 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1281 GGTCACC G^GT[ACGT]ACC
EcoT22I 382 ATGCAT ATGCA^T
FbaI 1337 TGATCA T^GATCA
HaeII 329 AGCGCT [GA]GCGC^[CT]
HgaI 1330 GACGC GACGC(5/10)
HhaI 330 GCGC GCG^C
Hin1I 1330 GACGCC G[GA]^CG[CT]C
HinP1I 330 GCGC G^CGC
HincII 1119 GTCAAC GT[CT]^[GA]AC
HindII 1119 GTCAAC GT[CT]^[GA]AC
HindIII 1027 AAGCTT A^AGCTT
Ksp632I 799 CTCTTC CTCTTC(1/4)
MaeI 391 CTAG C^TAG
MamI 1338 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 653 TCCGGA T^CCGGA
NaeI 1042 GCCGGC GCC^GGC
NcoI 593 CCATGG C^CATGG
NgoMIV 1042 GCCGGC G^CCGGC
NruI 601 TCGCGA TCG^CGA
NsiI 382 ATGCAT ATGCA^T
PflFI 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 16 AGTACT AGT^ACT
SmaI 1112 CCCGGG CCC^GGG
SmlI 1399 CTCAAG C^T[CT][GA]AG
SphI 701 GCATGC GCATG^C
SspBI 491 TGTACA T^GTACA
StuI 173 AGGCCT AGG^CCT
Tth111I 1210 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 890 CCATCCTGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1112 CCCGGG C^CCGGG
XmaI 1112 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 243 CGCG CG^CG
602 CGCG
BalI 689 TGGCCA TGG^CCA
1187 TGGCCA
BbvI 639 GCTGC GCAGC(8/12)
1395 GCTGC
BcnI 1112 CCCGG CC^[GC]GG
1113 CCGGG
BpmI 415 CTCCAG CTGGAG(16/14)
1276 CTGGAG
BspMI 953 ACCTGC ACCTGC(4/8)
995 ACCTGC
BsrDI 288 CATTGC GCAATG(2/0)
1038 CATTGC
BsrFI 761 GCCGGT [GA]^CCGG[CT]
1042 GCCGGC
BstUI 243 CGCG CG^CG
602 CGCG
BstXI 44 CCATCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
80 CCAAGAAGTTGG
Cfr10I 761 GCCGGT [GA]^CCGG[CT]
1042 GCCGGC
EaeI 689 TGGCCA [CT]^GGCC[GA]
1187 TGGCCA
EcoT14I 152 CCAAGG C^C[AT][AT]GG
593 CCATGG
MaeII 1076 ACGT A^CGT
1288 ACGT
MluNI 689 TGGCCA TGG^CCA
1187 TGGCCA
MscI 689 TGGCCA TGG^CCA
1187 TGGCCA
MvnI 243 CGCG CG^CG
602 CGCG
NciI 1112 CCCGG CC^[GC]GG
1113 CCGGG
PflMI 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCAGGCCCTGG
PpuMI 24 GGGTCCC [GA]G^G[AT]CC[CT]
1372 GGGACCT
PstI 1066 CTGCAG CTGCA^G
1082 CTGCAG
SexAI 185 ACCTGGT A^CC[AT]GGT
1211 ACCTGGT
SfcI 1066 CTGCAG C^T[GA][CT]AG
1082 CTGCAG
StyI 152 CCAAGG C^C[AT][AT]GG
593 CCATGG
TseI 639 GCTGC G^C[AT]GC
1395 GCTGC
Tsp509I 50 AATT ^AATT
514 AATT
Van91I 404 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1380 CCAGGCCCTGG
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BmyI BpuAI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I Tsp45I TspRI XhoII