Restriction map for
pFN21AE5732
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI BamHI
BbvCI BcgI BfrI BsaBI BseAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I HgaI HindIII
HpaI KpnI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SbfI SexAI SfiI SfuI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 778 CGCG CG^CG
Alw44I 1816 GTGCAC G^TGCAC
ApaLI 1816 GTGCAC G^TGCAC
AviII 1823 TGCGCA TGC^GCA
AvrII 306 CCTAGG C^CTAGG
BbrPI 1404 CACGTG CAC^GTG
BciVI 692 GTATCC GTATCC(6/5)
BclI 404 TGATCA T^GATCA
BglI 1777 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 881 AGATCT A^GATCT
BlnI 306 CCTAGG C^CTAGG
BlpI 1603 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1603 GCTGAGC GC^T[ACGT]AGC
BsaI 1213 GAGACC GGTCTC(1/5)
BsaWI 1757 ACCGGA [AT]^CCGG[AT]
BsiEI 1720 CGGCCG CG[GA][CT]^CG
BsmI 1433 GCATTC GAATGC(1/-1)
BspHI 1643 TCATGA T^CATGA
BspLU11I 1040 ACATGT A^CATGT
BsrBI 1699 GAGCGG CCGCTC(-3/-3)
BssSI 226 CTCGTG CACGAG(-5/-1)
BstUI 778 CGCG CG^CG
CelII 1603 GCTGAGC GC^T[ACGT]AGC
DraIII 1401 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1720 CGGCCG C^GGCCG
EclXI 1720 CGGCCG C^GGCCG
Eco52I 1720 CGGCCG C^GGCCG
FauI 1593 CCCGC CCCGC(4/6)
FbaI 404 TGATCA T^GATCA
FseI 1721 GGCCGGCC GGCCGG^CC
FspI 1823 TGCGCA TGC^GCA
HincII 79 GTCGAC GT[CT]^[GA]AC
HindII 79 GTCGAC GT[CT]^[GA]AC
KspI 777 CCGCGG CCGC^GG
MvnI 778 CGCG CG^CG
NcoI 1727 CCATGG C^CATGG
PmaCI 1404 CACGTG CAC^GTG
PmlI 1404 CACGTG CAC^GTG
RcaI 1643 TCATGA T^CATGA
SacII 777 CCGCGG CCGC^GG
SalI 79 GTCGAC G^TCGAC
ScaI 575 AGTACT AGT^ACT
SgrAI 351 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 697 CTCAAG C^T[CT][GA]AG
SphI 1658 GCATGC GCATG^C
StuI 165 AGGCCT AGG^CCT
XcmI 1298 CCATGTACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 79 GTCGAC GT^[AC][GT]AC
1108 GTCTAC
AcyI 217 GGCGCC G[GA]^CG[CT]C
349 GGCGCC
AflIII 938 ACACGT A^C[GA][CT]GT
1040 ACATGT
ApaI 310 GGGCCC GGGCC^C
530 GGGCCC
BanI 217 GGCGCC G^G[CT][GA]CC
349 GGCGCC
BbeI 217 GGCGCC GGCGC^C
349 GGCGCC
BbsI 711 GTCTTC GAAGAC(2/6)
849 GTCTTC
BcnI 108 CCGGG CC^[GC]GG
1480 CCGGG
BfaI 307 CTAG C^TAG
1189 CTAG
BpuAI 711 GTCTTC GAAGAC(2/6)
849 GTCTTC
BsaAI 415 TACGTG [CT]AC^GT[GA]
1404 CACGTG
BsaHI 217 GGCGCC G[GA]^CG[CT]C
349 GGCGCC
BsgI 101 CTGCAC GTGCAG(16/14)
552 CTGCAC
BsmFI 1737 GGGAC GGGAC(10/14)
1515 GTCCC
BstYI 635 AGATCC [GA]^GATC[CT]
881 AGATCT
DsaI 777 CCGCGG C^C[GA][CT]GG
1727 CCATGG
Hin1I 217 GGCGCC G[GA]^CG[CT]C
349 GGCGCC
KasI 217 GGCGCC G^GCGCC
349 GGCGCC
MaeI 307 CTAG C^TAG
1189 CTAG
MflI 635 AGATCC [GA]^GATC[CT]
881 AGATCT
NarI 217 GGCGCC GG^CGCC
349 GGCGCC
NciI 108 CCGGG CC^[GC]GG
1480 CCGGG
PflMI 854 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCATGGACTGG
PvuII 1226 CAGCTG CAG^CTG
1652 CAGCTG
SacI 17 GAGCTC GAGCT^C
280 GAGCTC
SapI 420 GCTCTTC GCTCTTC(1/4)
874 GCTCTTC
SfcI 957 CTATAG C^T[GA][CT]AG
1365 CTACAG
SfoI 217 GGCGCC GGC^GCC
349 GGCGCC
Van91I 854 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCATGGACTGG
XhoII 635 AGATCC [GA]^GATC[CT]
881 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NdeII NgoMIV
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I