Restriction map for
pFN21AE2683
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BsrDI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MfeI MluI MroI
MslI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AgeI 2843 ACCGGT A^CCGGT
AspI 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1851 GGATCC G^GATCC
BglII 553 AGATCT A^GATCT
BlpI 2919 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2919 GCTGAGC GC^T[ACGT]AGC
BsaWI 2843 ACCGGT [AT]^CCGG[AT]
BsgI 398 CTGCAC GTGCAG(16/14)
BsmBI 356 GAGACG CGTCTC(1/5)
BssHII 20 GCGCGC G^CGCGC
BstAPI 3057 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2591 GGTCACC G^GT[ACGT]ACC
BstPI 2591 GGTCACC G^GT[ACGT]ACC
BstXI 290 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2919 GCTGAGC GC^T[ACGT]AGC
DrdI 2470 GACTCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2591 GGTCACC G^GT[ACGT]ACC
HincII 563 GTCAAC GT[CT]^[GA]AC
HindII 563 GTCAAC GT[CT]^[GA]AC
MseI 578 TTAA T^TAA
NaeI 1262 GCCGGC GCC^GGC
NgoMIV 1262 GCCGGC G^CCGGC
PflFI 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1747 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2843 ACCGGT A^CCGGT
PshAI 319 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 678 CCTGCAGG CCTGCA^GG
SmaI 791 CCCGGG CCC^GGG
Sse8387I 678 CCTGCAGG CCTGCA^GG
TaqI 2604 TCGA T^CGA
TfiI 2971 GATTC G^A[AT]TC
Tru9I 578 TTAA T^TAA
Tsp509I 2977 AATT ^AATT
Tth111I 2365 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2604 TCGA T^CGA
Van91I 1747 CCAACTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 791 CCCGGG C^CCGGG
XmaI 791 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
ApaI 1210 GGGCCC GGGCC^C
1458 GGGCCC
BbeI 536 GGCGCC GGCGC^C
2236 GGCGCC
BfaI 802 CTAG C^TAG
1882 CTAG
Bsp1407I 637 TGTACA T^GTACA
2711 TGTACA
BsrBI 2556 CCGCTC CCGCTC(-3/-3)
1555 GAGCGG
BsrGI 637 TGTACA T^GTACA
2711 TGTACA
BsrI 2162 ACTGG ACTGG(1/-1)
219 CCAGT
KasI 536 GGCGCC G^GCGCC
2236 GGCGCC
MaeI 802 CTAG C^TAG
1882 CTAG
MaeIII 1734 GTCAC ^GT[ACGT]AC
2592 GTCAC
NarI 536 GGCGCC GG^CGCC
2236 GGCGCC
NcoI 1381 CCATGG C^CATGG
3023 CCATGG
SfoI 536 GGCGCC GGC^GCC
2236 GGCGCC
SspBI 637 TGTACA T^GTACA
2711 TGTACA
Tsp45I 1734 GTCAC ^GT[GC]AC
2592 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TseI TspRI
XhoII