Restriction map for
pFN21AE9552
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccI AccII AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI Asp718I AspEI AspHI AspI
AvaI AvaII AvrII BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpmI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I CpoI DraI
DraII DraIII DsaI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HgaI HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MfeI MluI MroI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SpeI Sse8387I SspBI StyI SwaI
TfiI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 298 GACGTC GACGT^C
AcyI 298 GACGTC G[GA]^CG[CT]C
AflIII 1065 ACATGT A^C[GA][CT]GT
Aor51HI 995 AGCGCT AGC^GCT
Asp700I 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 407 TGCGCA TGC^GCA
BcnI 914 CCGGG CC^[GC]GG
BlpI 1147 GCTCAGC GC^T[ACGT]AGC
BmyI 815 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 1147 GCTCAGC GC^T[ACGT]AGC
BsaAI 543 TACGTA [CT]AC^GT[GA]
BsaBI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 298 GACGTC G[GA]^CG[CT]C
BsaI 760 GAGACC GGTCTC(1/5)
BsmAI 760 GAGAC GTCTC(1/5)
Bsp1286I 815 GGGCAC G[AGT]GC[ACT]^C
BspDI 318 ATCGAT AT^CGAT
BspLU11I 1065 ACATGT A^CATGT
BstNI 868 CCTGG CC^[AT]GG
CelII 1147 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1020 GGGCC G^G[ACGT]CC
ClaI 318 ATCGAT AT^CGAT
DrdI 856 GACTACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 995 AGCGCT AGC^GCT
EcoRII 868 CCTGG ^CC[AT]GG
FauI 1119 CCCGC CCCGC(4/6)
FspI 407 TGCGCA TGC^GCA
HaeII 995 AGCGCT [GA]GCGC^[CT]
HapII 914 CCGG C^CGG
Hin1I 298 GACGTC G[GA]^CG[CT]C
HincII 562 GTCAAC GT[CT]^[GA]AC
HindII 562 GTCAAC GT[CT]^[GA]AC
HinfI 1107 GAGTC G^A[ACGT]TC
HpaII 914 CCGG C^CGG
MamI 93 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 786 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 914 CCGG C^CGG
MvaI 868 CCTGG CC^[AT]GG
NciI 914 CCGGG CC^[GC]GG
PflMI 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1107 GAGTC GAGTC(4/5)
Sau96I 1020 GGGCC G^G[ACGT]CC
SfcI 384 CTATAG C^T[GA][CT]AG
SnaBI 543 TACGTA TAC^GTA
SphI 663 GCATGC GCATG^C
SspI 835 AATATT AAT^ATT
StuI 715 AGGCCT AGG^CCT
Van91I 372 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1004 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AfaI 546 GTAC GT^AC
1069 GTAC
AseI 417 ATTAAT AT^TAAT
1051 ATTAAT
AsnI 417 ATTAAT AT^TAAT
1051 ATTAAT
BalI 95 TGGCCA TGG^CCA
1095 TGGCCA
BamHI 843 GGATCC G^GATCC
1057 GGATCC
BanI 229 GGTGCC G^G[CT][GA]CC
816 GGCACC
BbsI 1004 GAAGAC GAAGAC(2/6)
553 GTCTTC
BpuAI 1004 GAAGAC GAAGAC(2/6)
553 GTCTTC
BsaJI 867 CCCTGG C^C[ACGT][ACGT]GG
868 CCTGGG
BsrI 1098 CCAGT ACTGG(1/-1)
1154 CCAGT
BssKI 868 CCTGG ^CC[ACGT]GG
914 CCGGG
BstXI 233 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
833 CCAATATTGTGG
CfoI 408 GCGC GCG^C
996 GCGC
EaeI 95 TGGCCA [CT]^GGCC[GA]
1095 TGGCCA
HhaI 408 GCGC GCG^C
996 GCGC
HinP1I 408 GCGC G^CGC
996 GCGC
MboII 1004 GAAGA GAAGA(8/7)
554 TCTTC
MluNI 95 TGGCCA TGG^CCA
1095 TGGCCA
MscI 95 TGGCCA TGG^CCA
1095 TGGCCA
MseI 418 TTAA T^TAA
1052 TTAA
MwoI 213 GCTGGTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
740 GCACTCATCGC
NspI 663 GCATGC [GA]CATG^[CT]
1065 ACATGT
PshBI 417 ATTAAT AT^TAAT
1051 ATTAAT
RsaI 546 GTAC GT^AC
1069 GTAC
ScrFI 868 CCTGG CC^[ACGT]GG
914 CCGGG
TaqI 79 TCGA T^CGA
319 TCGA
Tru9I 418 TTAA T^TAA
1052 TTAA
TthHB8I 79 TCGA T^CGA
319 TCGA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AlwI BbvI BseRI
BsiYI BslI BsmFI BspMI BstF5I
BstYI Cac8I DdeI DpnII Fnu4HI
FokI HaeIII HphI ItaI MaeII
MaeIII MboI MflI MnlI NdeII
NlaIII NlaIV Sau3AI SfaNI TseI
Tsp45I Tsp509I TspRI XhoII