##################################
# NO CUT ENZYMES (  216 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MamI        MfeI        MflI        MluI        MnlI        
MroI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AlwNI          59  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI          122  TGGCCA               TGG^CCA
BciVI         111  GTATCC               GTATCC(6/5)
BfaI           68  CTAG                 C^TAG
BsaI           16  GGTCTC               GGTCTC(1/5)
BsiYI         151  CCAGTTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          151  CCAGTTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I       31  TGTACA               T^GTACA
BsrGI          31  TGTACA               T^GTACA
BstF5I        126  CATCC                GGATG(2/0)
DdeI           57  CTCAG                C^T[ACGT]AG
DpnII           6  GATC                 ^GATC
EaeI          122  TGGCCA               [CT]^GGCC[GA]
EarI          224  GAAGAG               CTCTTC(1/4)
FokI          126  CATCC                GGATG(9/13)
HaeIII        123  GGCC                 GG^CC
HphI          173  TCACC                GGTGA(8/7)
Ksp632I       224  GAAGAG               CTCTTC(1/4)
MaeI           68  CTAG                 C^TAG
MaeIII        191  GTCAC                ^GT[ACGT]AC
MboI            6  GATC                 ^GATC
MluNI         122  TGGCCA               TGG^CCA
MscI          122  TGGCCA               TGG^CCA
NdeII           6  GATC                 ^GATC
PflMI         151  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           93  CTGCAG               CTGCA^G
Sau3AI          6  GATC                 ^GATC
SspBI          31  TGTACA               T^GTACA
Tsp45I        191  GTCAC                ^GT[GC]AC
Van91I        151  CCAGTTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

AfaI           32  GTAC                 GT^AC
              200  GTAC                
BsmAI          17  GTCTC                GTCTC(1/5)
               75  GTCTC               
RsaI           32  GTAC                 GT^AC
              200  GTAC                
SfcI           48  CTGTAG               C^T[GA][CT]AG
               93  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AluI        BbvI        BsrI        Fnu4HI      ItaI        
MboII       TseI        TspRI