Restriction map for
pFN21AE5363
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AviII 386 TGCGCA TGC^GCA
BbeI 281 GGCGCC GGCGC^C
BbrPI 591 CACGTG CAC^GTG
BbsI 129 GAAGAC GAAGAC(2/6)
BfaI 167 CTAG C^TAG
BlpI 563 GCTCAGC GC^T[ACGT]AGC
BpmI 91 CTCCAG CTGGAG(16/14)
Bpu1102I 563 GCTCAGC GC^T[ACGT]AGC
BpuAI 129 GAAGAC GAAGAC(2/6)
BsaAI 591 CACGTG [CT]AC^GT[GA]
BsiEI 795 CGACCG CG[GA][CT]^CG
BspMI 752 ACCTGC ACCTGC(4/8)
BsrBI 681 CCGCTC CCGCTC(-3/-3)
BstAPI 462 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 134 CCAGCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 266 GGATCT [GA]^GATC[CT]
CelII 563 GCTCAGC GC^T[ACGT]AGC
DrdI 244 GACCAGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 371 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 386 TGCGCA TGC^GCA
HincII 580 GTCAAC GT[CT]^[GA]AC
HindII 580 GTCAAC GT[CT]^[GA]AC
KasI 281 GGCGCC G^GCGCC
MaeI 167 CTAG C^TAG
MflI 266 GGATCT [GA]^GATC[CT]
MslI 591 CACGTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 86 GCCGGC GCC^GGC
NarI 281 GGCGCC GG^CGCC
NgoMIV 86 GCCGGC G^CCGGC
NspI 596 GCATGT [GA]CATG^[CT]
PmaCI 591 CACGTG CAC^GTG
PmlI 591 CACGTG CAC^GTG
PshAI 573 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 122 ACCAGGT A^CC[AT]GGT
SfoI 281 GGCGCC GGC^GCC
SmaI 200 CCCGGG CCC^GGG
SmlI 427 CTCAAG C^T[CT][GA]AG
XcmI 38 CCAACTCAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 266 GGATCT [GA]^GATC[CT]
XmaCI 200 CCCGGG C^CCGGG
XmaI 200 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 228 GACGCC G[GA]^CG[CT]C
281 GGCGCC
AlwI 12 GATCC GGATC(4/5)
266 GGATC
AlwNI 415 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
571 CAGACTCTG
ApaI 209 GGGCCC GGGCC^C
432 GGGCCC
AspHI 561 GTGCTC G[AT]GC[AT]^C
716 GTGCTC
AvaI 200 CCCGGG C^[CT]CG[GA]G
448 CCCGAG
BanI 281 GGCGCC G^G[CT][GA]CC
345 GGCACC
BsaHI 228 GACGCC G[GA]^CG[CT]C
281 GGCGCC
BsgI 627 CTGCAC GTGCAG(16/14)
607 GTGCAG
BsiHKAI 561 GTGCTC G[AT]GC[AT]^C
716 GTGCTC
BsmFI 78 GTCCC GGGAC(10/14)
442 GTCCC
BsoBI 200 CCCGGG C^[CT]CG[GA]G
448 CCCGAG
DpnII 12 GATC ^GATC
267 GATC
DsaI 356 CCGTGG C^C[GA][CT]GG
725 CCGTGG
EcoT14I 376 CCAAGG C^C[AT][AT]GG
398 CCAAGG
HaeII 22 AGCGCC [GA]GCGC^[CT]
281 GGCGCC
HgaI 228 GACGC GACGC(5/10)
839 GCGTC
Hin1I 228 GACGCC G[GA]^CG[CT]C
281 GGCGCC
HinfI 33 GACTC G^A[ACGT]TC
573 GACTC
MaeII 523 ACGT A^CGT
592 ACGT
MaeIII 242 GTGAC ^GT[ACGT]AC
253 GTCAC
MboI 12 GATC ^GATC
267 GATC
MboII 129 GAAGA GAAGA(8/7)
617 TCTTC
NdeII 12 GATC ^GATC
267 GATC
PflMI 414 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGACTCTGG
PleI 33 GACTC GAGTC(4/5)
573 GACTC
Sau3AI 12 GATC ^GATC
267 GATC
StuI 368 AGGCCT AGG^CCT
479 AGGCCT
StyI 376 CCAAGG C^C[AT][AT]GG
398 CCAAGG
Tsp45I 242 GTGAC ^GT[GC]AC
253 GTCAC
TspRI 409 CACTG CA[GC]TG[ACGT][ACGT]^
625 CACTG
Van91I 414 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
570 CCAGACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BsrFI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI