Restriction map for
pFN21AE5840
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AgeI Aor51HI ApaI AscI Asp700I
AviII AvrII BbeI BlnI BlpI
Bpu1102I BsiWI BsmBI Bsp1407I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I CelII Cfr10I CpoI
DrdI Eco47III EcoNI EcoRV FseI
FspI KasI KspI MluI NaeI
NarI NgoMIV NotI NruI NspV
PacI PinAI PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI SwaI XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 3398 GACGTC GACGT^C
AccIII 515 TCCGGA T^CCGGA
AcyI 3398 GACGTC G[GA]^CG[CT]C
AflII 2401 CTTAAG C^TTAAG
AspI 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2034 TGGCCA TGG^CCA
BbrPI 2147 CACGTG CAC^GTG
BcgI 5592 GCAACAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2401 CTTAAG C^TTAAG
BglII 333 AGATCT A^GATCT
BsaAI 2147 CACGTG [CT]AC^GT[GA]
BsaBI 4542 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3398 GACGTC G[GA]^CG[CT]C
BseAI 515 TCCGGA T^CCGGA
BspDI 3108 ATCGAT AT^CGAT
BspEI 515 TCCGGA T^CCGGA
BssSI 6704 CACGAG CACGAG(-5/-1)
BstEII 6851 GGTTACC G^GT[ACGT]ACC
BstPI 6851 GGTTACC G^GT[ACGT]ACC
ClaI 3108 ATCGAT AT^CGAT
DraIII 3051 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 7651 CGGCCG C^GGCCG
EclXI 7651 CGGCCG C^GGCCG
Eco52I 7651 CGGCCG C^GGCCG
EcoO65I 6851 GGTTACC G^GT[ACGT]ACC
Hin1I 3398 GACGTC G[GA]^CG[CT]C
HpaI 1879 GTTAAC GTT^AAC
MamI 4542 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 7100 CAATTG C^AATTG
MluNI 2034 TGGCCA TGG^CCA
MroI 515 TCCGGA T^CCGGA
MscI 2034 TGGCCA TGG^CCA
MunI 7100 CAATTG C^AATTG
NdeI 4693 CATATG CA^TATG
NheI 1329 GCTAGC G^CTAGC
PflFI 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2147 CACGTG CAC^GTG
PmlI 2147 CACGTG CAC^GTG
PshAI 3901 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 6179 GAGCTC GAGCT^C
SapI 2473 GAAGAGC GCTCTTC(1/4)
SpeI 6838 ACTAGT A^CTAGT
StuI 1232 AGGCCT AGG^CCT
Tth111I 4986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 32 enzymes) #
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Acc65I 5389 GGTACC G^GTACC
6438 GGTACC
AccI 3412 GTAGAC GT^[AC][GT]AC
5735 GTCTAC
AccII 62 CGCG CG^CG
216 CGCG
AclI 5460 AACGTT AA^CGTT
7044 AACGTT
AhdI 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
Alw44I 542 GTGCAC G^TGCAC
1842 GTGCAC
ApaLI 542 GTGCAC G^TGCAC
1842 GTGCAC
AseI 3541 ATTAAT AT^TAAT
3646 ATTAAT
AsnI 3541 ATTAAT AT^TAAT
3646 ATTAAT
Asp718I 5389 GGTACC G^GTACC
6438 GGTACC
AspEI 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
BanII 3944 GAGCCC G[GA]GC[CT]^C
6179 GAGCTC
BbsI 5311 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BpuAI 5311 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BsaI 3661 GAGACC GGTCTC(1/5)
5679 GAGACC
BsaWI 515 TCCGGA [AT]^CCGG[AT]
4067 ACCGGA
BsiEI 7651 CGGCCG CG[GA][CT]^CG
7696 CGACCG
BstUI 62 CGCG CG^CG
216 CGCG
DraI 2710 TTTAAA TTT^AAA
4293 TTTAAA
EaeI 2034 TGGCCA [CT]^GGCC[GA]
7651 CGGCCG
Eam1105I 292 GACGAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACACAGTGTC
HindIII 491 AAGCTT A^AGCTT
632 AAGCTT
KpnI 5389 GGTACC GGTAC^C
6438 GGTACC
MvnI 62 CGCG CG^CG
216 CGCG
PaeR7I 3428 CTCGAG C^TCGAG
6392 CTCGAG
PflMI 2316 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6105 CCAGCCCATGG
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
2288 AGGACCT
PshBI 3541 ATTAAT AT^TAAT
3646 ATTAAT
Psp1406I 5460 AACGTT AA^CGTT
7044 AACGTT
SphI 780 GCATGC GCATG^C
1683 GCATGC
Van91I 2316 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6105 CCAGCCCATGG
XhoI 3428 CTCGAG C^TCGAG
6392 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BamHI BanI BbvCI
BbvI BciVI BclI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I EcoT22I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmaI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI