Restriction map for
pFN21AE5270
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII BamHI BbeI BbrPI BbvCI
BclI BfrI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KspI MaeII MamI MfeI
MluI MroI MseI MslI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 554 GGTACC G^GTACC
AfaI 555 GTAC GT^AC
Asp718I 554 GGTACC G^GTACC
AvaI 405 CCCGGG C^[CT]CG[GA]G
AvrII 156 CCTAGG C^CTAGG
BalI 248 TGGCCA TGG^CCA
BanI 554 GGTACC G^G[CT][GA]CC
BbsI 323 GTCTTC GAAGAC(2/6)
BcgI 43 GCAGTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 133 GTATCC GTATCC(6/5)
BglI 490 GCCGTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 201 AGATCT A^GATCT
BlnI 156 CCTAGG C^CTAGG
BlpI 435 GCTGAGC GC^T[ACGT]AGC
BpmI 76 CTGGAG CTGGAG(16/14)
Bpu1102I 435 GCTGAGC GC^T[ACGT]AGC
BpuAI 323 GTCTTC GAAGAC(2/6)
BsaWI 173 ACCGGA [AT]^CCGG[AT]
BsiEI 165 CGGCCG CG[GA][CT]^CG
BsmI 358 GAATGC GAATGC(1/-1)
BsoBI 405 CCCGGG C^[CT]CG[GA]G
BsrBI 79 GAGCGG CCGCTC(-3/-3)
BstEII 409 GGTTACC G^GT[ACGT]ACC
BstPI 409 GGTTACC G^GT[ACGT]ACC
BstYI 201 AGATCT [GA]^GATC[CT]
Cac8I 96 GCTAGC GC[ACGT]^[ACGT]GC
CelII 435 GCTGAGC GC^T[ACGT]AGC
DdeI 436 CTGAG C^T[ACGT]AG
DpnII 202 GATC ^GATC
DraII 314 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 172 GACCGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 165 CGGCCG C^GGCCG
EclXI 165 CGGCCG C^GGCCG
Eco52I 165 CGGCCG C^GGCCG
EcoO109I 314 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 409 GGTTACC G^GT[ACGT]ACC
HinfI 218 GAGTC G^A[ACGT]TC
HphI 60 GGTGA GGTGA(8/7)
KpnI 554 GGTACC GGTAC^C
MboI 202 GATC ^GATC
MflI 201 AGATCT [GA]^GATC[CT]
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MspA1I 145 CAGCTG C[AC]G^C[GT]G
MwoI 490 GCCGTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 202 GATC ^GATC
NheI 96 GCTAGC G^CTAGC
PleI 218 GAGTC GAGTC(4/5)
PpuMI 314 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 316 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 41 CTGCAG CTGCA^G
PvuII 145 CAGCTG CAG^CTG
RsaI 555 GTAC GT^AC
SacI 376 GAGCTC GAGCT^C
Sau3AI 202 GATC ^GATC
SfcI 41 CTGCAG C^T[GA][CT]AG
SmaI 405 CCCGGG CCC^GGG
Tsp509I 616 AATT ^AATT
XcmI 482 CCATCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 201 AGATCT [GA]^GATC[CT]
XmaCI 405 CCCGGG C^CCGGG
XmaI 405 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 81 GCGG CCGC(-3/-1)
164 GCGG
AspHI 268 GAGCAC G[AT]GC[AT]^C
376 GAGCTC
BanII 376 GAGCTC G[GA]GC[CT]^C
383 GGGCTC
BseRI 265 GAGGAG GAGGAG(10/8)
566 GAGGAG
BsiHKAI 268 GAGCAC G[AT]GC[AT]^C
376 GAGCTC
BsmFI 170 GGGAC GGGAC(10/14)
314 GGGAC
BstF5I 348 CATCC GGATG(2/0)
483 CATCC
BstNI 75 CCTGG CC^[AT]GG
486 CCTGG
EarI 309 GAAGAG CTCTTC(1/4)
589 GAAGAG
EcoRII 75 CCTGG ^CC[AT]GG
486 CCTGG
FokI 348 CATCC GGATG(9/13)
483 CATCC
Ksp632I 309 GAAGAG CTCTTC(1/4)
589 GAAGAG
MvaI 75 CCTGG CC^[AT]GG
486 CCTGG
NcoI 351 CCATGG C^CATGG
414 CCATGG
SfaNI 347 GCATC GCATC(5/9)
395 GCATC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AvaII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI Cfr13I DsaI EaeI
EcoT14I Fnu4HI HaeIII HapII HpaII
ItaI MaeI MaeIII MboII MnlI
MspI NciI NlaIII NlaIV Sau96I
ScrFI StyI TseI Tsp45I TspRI