Restriction map for
pFN21AE1635
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
Acc65I AccI AccII AclI AcsI
AflII AgeI AhdI AluI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstF5I BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
Fnu4HI FokI FseI FspI HaeII
HgaI HhaI HinP1I HincII HindII
HindIII HpaI ItaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MspA1I MunI MvnI
MwoI NaeI NarI NciI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfaNI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
TseI Tth111I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 169 GACGTC GACGT^C
AccIII 98 TCCGGA T^CCGGA
AcyI 169 GACGTC G[GA]^CG[CT]C
AflIII 103 ACGTGT A^C[GA][CT]GT
Alw44I 409 GTGCAC G^TGCAC
ApaLI 409 GTGCAC G^TGCAC
AvaI 429 CTCGAG C^[CT]CG[GA]G
AvaII 334 GGTCC G^G[AT]CC
BbsI 84 GTCTTC GAAGAC(2/6)
BpuAI 84 GTCTTC GAAGAC(2/6)
BsaHI 169 GACGTC G[GA]^CG[CT]C
BsaWI 98 TCCGGA [AT]^CCGG[AT]
BseAI 98 TCCGGA T^CCGGA
BsmI 252 GAATGC GAATGC(1/-1)
BsoBI 429 CTCGAG C^[CT]CG[GA]G
BspEI 98 TCCGGA T^CCGGA
BstBI 272 TTCGAA TT^CGAA
BstXI 114 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 76 GCTGGC GC[ACGT]^[ACGT]GC
DraII 333 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 284 GAAGAG CTCTTC(1/4)
EcoO109I 333 AGGTCCT [GA]G^G[ACGT]CC[CT]
HapII 99 CCGG C^CGG
Hin1I 169 GACGTC G[GA]^CG[CT]C
HinfI 427 GACTC G^A[ACGT]TC
HpaII 99 CCGG C^CGG
HphI 156 TCACC GGTGA(8/7)
Ksp632I 284 GAAGAG CTCTTC(1/4)
MroI 98 TCCGGA T^CCGGA
MspI 99 CCGG C^CGG
NspI 405 GCATGT [GA]CATG^[CT]
NspV 272 TTCGAA TT^CGAA
PaeR7I 429 CTCGAG C^TCGAG
PleI 427 GACTC GAGTC(4/5)
PpuMI 333 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 128 AGTACT AGT^ACT
SfuI 272 TTCGAA TT^CGAA
SmlI 429 CTCGAG C^T[CT][GA]AG
StuI 151 AGGCCT AGG^CCT
Tsp509I 321 AATT ^AATT
XhoI 429 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AciI 44 CCGC CCGC(-3/-1)
111 CCGC
AfaI 129 GTAC GT^AC
198 GTAC
AlwI 96 GATCC GGATC(4/5)
187 GGATC
AspHI 177 GTGCTC G[AT]GC[AT]^C
409 GTGCAC
BmyI 177 GTGCTC G[AGT]GC[ACT]^C
409 GTGCAC
BsiHKAI 177 GTGCTC G[AT]GC[AT]^C
409 GTGCAC
Bsp1286I 177 GTGCTC G[AGT]GC[ACT]^C
409 GTGCAC
BsrI 48 ACTGG ACTGG(1/-1)
211 ACTGG
BstYI 95 AGATCC [GA]^GATC[CT]
187 GGATCT
DpnII 96 GATC ^GATC
188 GATC
DsaI 58 CCATGG C^C[GA][CT]GG
114 CCATGG
EcoT14I 58 CCATGG C^C[AT][AT]GG
114 CCATGG
MaeII 103 ACGT A^CGT
170 ACGT
MboI 96 GATC ^GATC
188 GATC
MboII 284 GAAGA GAAGA(8/7)
85 TCTTC
MflI 95 AGATCC [GA]^GATC[CT]
187 GGATCT
NcoI 58 CCATGG C^CATGG
114 CCATGG
NdeII 96 GATC ^GATC
188 GATC
PflMI 159 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
205 CCATTCACTGG
RsaI 129 GTAC GT^AC
198 GTAC
Sau3AI 96 GATC ^GATC
188 GATC
StyI 58 CCATGG C^C[AT][AT]GG
114 CCATGG
TaqI 273 TCGA T^CGA
430 TCGA
TspRI 47 CACTG CA[GC]TG[ACGT][ACGT]^
210 CACTG
TthHB8I 273 TCGA T^CGA
430 TCGA
Van91I 159 CCAGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
205 CCATTCACTGG
XhoII 95 AGATCC [GA]^GATC[CT]
187 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BsaJI BsiYI BslI BssKI BstNI
Cfr13I EcoRII HaeIII MaeIII MnlI
MseI MslI MvaI NlaIII Sau96I
ScrFI Tru9I Tsp45I