Restriction map for
pFN21AE1789
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstNI BstPI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRII EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI MvaI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tsp45I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 826 GTCTAC GT^[AC][GT]AC
AccII 168 CGCG CG^CG
AlwI 137 GGATC GGATC(4/5)
AspI 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 157 CCCGGG C^[CT]CG[GA]G
BbsI 808 GTCTTC GAAGAC(2/6)
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BciVI 783 GGATAC GTATCC(6/5)
BfaI 454 CTAG C^TAG
BglII 816 AGATCT A^GATCT
BpuAI 808 GTCTTC GAAGAC(2/6)
BsaWI 134 ACCGGA [AT]^CCGG[AT]
BsmAI 332 GAGAC GTCTC(1/5)
BsoBI 157 CCCGGG C^[CT]CG[GA]G
BspDI 773 ATCGAT AT^CGAT
BsrBI 162 GAGCGG CCGCTC(-3/-3)
BsrDI 484 GCAATG GCAATG(2/0)
BstUI 168 CGCG CG^CG
BstXI 291 CCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 816 AGATCT [GA]^GATC[CT]
Cac8I 52 GCGGGC GC[ACGT]^[ACGT]GC
ClaI 773 ATCGAT AT^CGAT
DsaI 447 CCATGG C^C[GA][CT]GG
EarI 590 CTCTTC CTCTTC(1/4)
EcoRI 752 GAATTC G^AATTC
EcoT14I 447 CCATGG C^C[AT][AT]GG
HincII 379 GTCAAC GT[CT]^[GA]AC
HindII 379 GTCAAC GT[CT]^[GA]AC
HphI 262 GGTGA GGTGA(8/7)
Ksp632I 590 CTCTTC CTCTTC(1/4)
MaeI 454 CTAG C^TAG
MflI 816 AGATCT [GA]^GATC[CT]
MseI 353 TTAA T^TAA
MslI 359 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 168 CGCG CG^CG
NcoI 447 CCATGG C^CATGG
PflFI 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 388 GAGCTC GAGCT^C
SapI 589 GCTCTTC GCTCTTC(1/4)
ScaI 723 AGTACT AGT^ACT
SfaNI 128 GCATC GCATC(5/9)
SfcI 789 CTATAG C^T[GA][CT]AG
SmaI 157 CCCGGG CCC^GGG
SmlI 385 CTTGAG C^T[CT][GA]AG
StyI 447 CCATGG C^C[AT][AT]GG
Tru9I 353 TTAA T^TAA
TspRI 207 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 424 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 816 AGATCT [GA]^GATC[CT]
XmaCI 157 CCCGGG C^CCGGG
XmaI 157 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 550 GAATTT [GA]^AATT[CT]
752 GAATTC
ApoI 550 GAATTT [GA]^AATT[CT]
752 GAATTC
AspHI 388 GAGCTC G[AT]GC[AT]^C
587 GTGCTC
BsgI 734 CTGCAC GTGCAG(16/14)
366 GTGCAG
BsiHKAI 388 GAGCTC G[AT]GC[AT]^C
587 GTGCTC
BspHI 110 TCATGA T^CATGA
852 TCATGA
BsrI 327 ACTGG ACTGG(1/-1)
521 CCAGT
BstBI 475 TTCGAA TT^CGAA
563 TTCGAA
BstF5I 531 GGATG GGATG(2/0)
583 GGATG
CfoI 126 GCGC GCG^C
167 GCGC
EaeI 11 TGGCCG [CT]^GGCC[GA]
305 TGGCCG
FokI 531 GGATG GGATG(9/13)
583 GGATG
HgaI 78 GCGTC GACGC(5/10)
93 GCGTC
HhaI 126 GCGC GCG^C
167 GCGC
HinP1I 126 GCGC G^CGC
167 GCGC
NspV 475 TTCGAA TT^CGAA
563 TTCGAA
PvuII 502 CAGCTG CAG^CTG
731 CAGCTG
RcaI 110 TCATGA T^CATGA
852 TCATGA
SfuI 475 TTCGAA TT^CGAA
563 TTCGAA
SspI 567 AATATT AAT^ATT
686 AATATT
TfiI 265 GATTC G^A[AT]TC
473 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI Cfr13I
DdeI DpnII DraII EcoO109I FauI
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspA1I
MspI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI RsaI Sau3AI
Sau96I ScrFI TaqI TseI Tsp509I
TthHB8I