Restriction map for
pFN21AB5872
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BbrPI BbvCI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrI Bst1107I BstBI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI Eam1105I EcoNI EcoRI
EcoRV FbaI FseI HgaI HincII
HindII HindIII HpaI KpnI MaeII
MamI MfeI MluI MluNI MroI
MscI MslI MunI NcoI NdeI
NheI NotI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tsp509I Tth111I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 361 GTCTAC GT^[AC][GT]AC
AcyI 223 GGCGCC G[GA]^CG[CT]C
AflII 877 CTTAAG C^TTAAG
Aor51HI 886 AGCGCT AGC^GCT
ApaI 230 GGGCCC GGGCC^C
AscI 52 GGCGCGCC GG^CGCGCC
AviII 270 TGCGCA TGC^GCA
BamHI 200 GGATCC G^GATCC
BanI 223 GGCGCC G^G[CT][GA]CC
BbeI 223 GGCGCC GGCGC^C
BbsI 756 GAAGAC GAAGAC(2/6)
BcgI 1139 CGATGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 658 CTAG C^TAG
BfrI 877 CTTAAG C^TTAAG
BpmI 307 CTGGAG CTGGAG(16/14)
BpuAI 756 GAAGAC GAAGAC(2/6)
BsaHI 223 GGCGCC G[GA]^CG[CT]C
BsaI 355 GAGACC GGTCTC(1/5)
BseRI 91 GAGGAG GAGGAG(10/8)
BsmI 1090 GCATTC GAATGC(1/-1)
BsrDI 861 CATTGC GCAATG(2/0)
BssHII 53 GCGCGC G^CGCGC
BssSI 163 CTCGTG CACGAG(-5/-1)
BstAPI 726 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 676 GGTCACC G^GT[ACGT]ACC
BstPI 676 GGTCACC G^GT[ACGT]ACC
DraIII 622 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 185 CGGCCG C^GGCCG
EclXI 185 CGGCCG C^GGCCG
Eco47III 886 AGCGCT AGC^GCT
Eco52I 185 CGGCCG C^GGCCG
EcoO65I 676 GGTCACC G^GT[ACGT]ACC
FspI 270 TGCGCA TGC^GCA
Hin1I 223 GGCGCC G[GA]^CG[CT]C
KasI 223 GGCGCC G^GCGCC
KspI 234 CCGCGG CCGC^GG
MaeI 658 CTAG C^TAG
MaeIII 677 GTCAC ^GT[ACGT]AC
MseI 878 TTAA T^TAA
NarI 223 GGCGCC GG^CGCC
NruI 931 TCGCGA TCG^CGA
PflMI 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 209 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 148 GAGCTC GAGCT^C
SacII 234 CCGCGG CCGC^GG
SapI 937 GAAGAGC GCTCTTC(1/4)
ScaI 950 AGTACT AGT^ACT
SexAI 672 ACCTGGT A^CC[AT]GGT
SfaNI 654 GATGC GCATC(5/9)
SfoI 223 GGCGCC GGC^GCC
StuI 553 AGGCCT AGG^CCT
TfiI 1063 GAATC G^A[AT]TC
Tru9I 878 TTAA T^TAA
Tsp45I 677 GTCAC ^GT[GC]AC
Van91I 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 333 GTGCAC G^TGCAC
870 GTGCAC
AlwNI 727 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
901 CAGATCCTG
AocI 596 CCTCAGG CC^T[ACGT]AGG
716 CCTCAGG
ApaLI 333 GTGCAC G^TGCAC
870 GTGCAC
AvaII 127 GGTCC G^G[AT]CC
210 GGTCC
BsmAI 276 GAGAC GTCTC(1/5)
355 GAGAC
Bsp1407I 347 TGTACA T^GTACA
833 TGTACA
BspMI 522 ACCTGC ACCTGC(4/8)
914 GCAGGT
BsrGI 347 TGTACA T^GTACA
833 TGTACA
BstYI 200 GGATCC [GA]^GATC[CT]
902 AGATCC
Bsu36I 596 CCTCAGG CC^T[ACGT]AGG
716 CCTCAGG
DdeI 597 CTCAG C^T[ACGT]AG
717 CTCAG
DpnII 201 GATC ^GATC
903 GATC
DsaI 234 CCGCGG C^C[GA][CT]GG
462 CCACGG
Eco81I 596 CCTCAGG CC^T[ACGT]AGG
716 CCTCAGG
EcoT14I 773 CCAAGG C^C[AT][AT]GG
854 CCAAGG
EcoT22I 385 ATGCAT ATGCA^T
1088 ATGCAT
HphI 638 TCACC GGTGA(8/7)
678 TCACC
MboI 201 GATC ^GATC
903 GATC
MflI 200 GGATCC [GA]^GATC[CT]
902 AGATCC
NaeI 187 GCCGGC GCC^GGC
220 GCCGGC
NdeII 201 GATC ^GATC
903 GATC
NgoMIV 187 GCCGGC G^CCGGC
220 GCCGGC
NsiI 385 ATGCAT ATGCA^T
1088 ATGCAT
NspI 344 GCATGT [GA]CATG^[CT]
986 ACATGC
PstI 559 CTGCAG CTGCA^G
733 CTGCAG
PvuII 7 CAGCTG CAG^CTG
979 CAGCTG
Sau3AI 201 GATC ^GATC
903 GATC
SfcI 559 CTGCAG C^T[GA][CT]AG
733 CTGCAG
SmaI 204 CCCGGG CCC^GGG
213 CCCGGG
SmlI 151 CTCAAG C^T[CT][GA]AG
877 CTTAAG
SspBI 347 TGTACA T^GTACA
833 TGTACA
StyI 773 CCAAGG C^C[AT][AT]GG
854 CCAAGG
TaqI 743 TCGA T^CGA
1045 TCGA
TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^
1145 CAGTG
TthHB8I 743 TCGA T^CGA
1045 TCGA
XhoII 200 GGATCC [GA]^GATC[CT]
902 AGATCC
XmaCI 204 CCCGGG C^CCGGG
213 CCCGGG
XmaI 204 CCCGGG C^CCGGG
213 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI BanII BbvI BcnI
BmyI BsaJI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
TseI