Restriction map for
pFN21AE1667
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaII AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KpnI Ksp632I KspI MaeII MaeIII
MamI MboI MflI MluI MluNI
MroI MscI MslI NaeI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I Tth111I Van91I
XbaI XcmI XhoII XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 99 CGCG CG^CG
AcyI 53 GGCGCC G[GA]^CG[CT]C
AluI 170 AGCT AG^CT
BanI 53 GGCGCC G^G[CT][GA]CC
BanII 238 GAGCCC G[GA]GC[CT]^C
BbeI 53 GGCGCC GGCGC^C
BbsI 246 GTCTTC GAAGAC(2/6)
BfaI 5 CTAG C^TAG
BmyI 238 GAGCCC G[AGT]GC[ACT]^C
BpuAI 246 GTCTTC GAAGAC(2/6)
BsaHI 53 GGCGCC G[GA]^CG[CT]C
BseRI 148 GAGGAG GAGGAG(10/8)
BsgI 94 CTGCAC GTGCAG(16/14)
BsiYI 44 CCCTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 44 CCCTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 40 GTCCC GGGAC(10/14)
Bsp1286I 238 GAGCCC G[AGT]GC[ACT]^C
BspMI 279 ACCTGC ACCTGC(4/8)
BsrBI 117 CCGCTC CCGCTC(-3/-3)
BsrI 525 ACTGG ACTGG(1/-1)
BstNI 74 CCTGG CC^[AT]GG
BstUI 99 CGCG CG^CG
BstXI 480 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRII 74 CCTGG ^CC[AT]GG
EcoT14I 384 CCAAGG C^C[AT][AT]GG
HaeII 53 GGCGCC [GA]GCGC^[CT]
HaeIII 426 GGCC GG^CC
Hin1I 53 GGCGCC G[GA]^CG[CT]C
KasI 53 GGCGCC G^GCGCC
MaeI 5 CTAG C^TAG
MboII 247 TCTTC GAAGA(8/7)
MfeI 182 CAATTG C^AATTG
MseI 416 TTAA T^TAA
MspA1I 251 CCGCTG C[AC]G^C[GT]G
MunI 182 CAATTG C^AATTG
MvaI 74 CCTGG CC^[AT]GG
MvnI 99 CGCG CG^CG
NarI 53 GGCGCC GG^CGCC
PaeR7I 122 CTCGAG C^TCGAG
PleI 389 GACTC GAGTC(4/5)
PstI 254 CTGCAG CTGCA^G
SfcI 254 CTGCAG C^T[GA][CT]AG
SfoI 53 GGCGCC GGC^GCC
SmaI 264 CCCGGG CCC^GGG
SmlI 122 CTCGAG C^T[CT][GA]AG
StyI 384 CCAAGG C^C[AT][AT]GG
TaqI 123 TCGA T^CGA
TfiI 453 GAATC G^A[AT]TC
Tru9I 416 TTAA T^TAA
TthHB8I 123 TCGA T^CGA
XhoI 122 CTCGAG C^TCGAG
XmaCI 264 CCCGGG C^CCGGG
XmaI 264 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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AvaI 122 CTCGAG C^[CT]CG[GA]G
264 CCCGGG
BpmI 75 CTGGAG CTGGAG(16/14)
191 CTGGAG
BsoBI 122 CTCGAG C^[CT]CG[GA]G
264 CCCGGG
BstF5I 381 CATCC GGATG(2/0)
210 GGATG
CfoI 54 GCGC GCG^C
100 GCGC
FokI 381 CATCC GGATG(9/13)
210 GGATG
HgaI 67 GACGC GACGC(5/10)
38 GCGTC
HhaI 54 GCGC GCG^C
100 GCGC
HinP1I 54 GCGC G^CGC
100 GCGC
HinfI 389 GACTC G^A[ACGT]TC
453 GAATC
MwoI 56 GCCTCCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
110 GCTACTTCCGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI BbvI BcnI BsaJI BsmAI
BssKI Cac8I DdeI Fnu4HI HapII
HpaII ItaI MnlI MspI NciI
NlaIV ScrFI TseI Tsp509I TspRI