Restriction map for
pFN21AB0466
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BbrPI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI BstEII BstPI Bsu36I CelII
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MluNI
MroI MscI MslI MunI NdeI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SexAI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Asp700I 997 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2549 GGATCC G^GATCC
BbsI 1190 GAAGAC GAAGAC(2/6)
BcgI 270 CGAGGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2067 GTATCC GTATCC(6/5)
BclI 1380 TGATCA T^GATCA
BpuAI 1190 GAAGAC GAAGAC(2/6)
BsaWI 143 ACCGGA [AT]^CCGG[AT]
BsmBI 19 GAGACG CGTCTC(1/5)
BsmI 1370 GAATGC GAATGC(1/-1)
BsrBI 165 GAGCGG CCGCTC(-3/-3)
BssHII 714 GCGCGC G^CGCGC
Bst1107I 1711 GTATAC GTA^TAC
BstBI 2048 TTCGAA TT^CGAA
BstXI 650 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1711 GTATAC GTA^TAC
DraI 2056 TTTAAA TTT^AAA
DraIII 849 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 21 GACGCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 912 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1380 TGATCA T^GATCA
MaeII 1836 ACGT A^CGT
NheI 2682 GCTAGC G^CTAGC
NspV 2048 TTCGAA TT^CGAA
PaeR7I 25 CTCGAG C^TCGAG
PflMI 882 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 1325 AGTACT AGT^ACT
SfuI 2048 TTCGAA TT^CGAA
SmlI 25 CTCGAG C^T[CT][GA]AG
TfiI 2833 GAATC G^A[AT]TC
Tsp45I 2162 GTGAC ^GT[GC]AC
Van91I 882 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 25 CTCGAG C^TCGAG
XmnI 997 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 82 AAATTC [GA]^AATT[CT]
1390 AAATTT
Alw44I 721 GTGCAC G^TGCAC
2609 GTGCAC
ApaI 238 GGGCCC GGGCC^C
307 GGGCCC
ApaLI 721 GTGCAC G^TGCAC
2609 GTGCAC
ApoI 82 AAATTC [GA]^AATT[CT]
1390 AAATTT
BbvCI 966 CCTCAGC CCTCAGC(-5/-2)
2573 CCTCAGC
BglI 248 GCCCGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
502 GCCCGGCCGGC
BglII 1311 AGATCT A^GATCT
2517 AGATCT
BsaI 934 GAGACC GGTCTC(1/5)
564 GGTCTC
BsgI 678 GTGCAG GTGCAG(16/14)
2129 GTGCAG
BsiEI 505 CGGCCG CG[GA][CT]^CG
668 CGGCCG
EaeI 505 CGGCCG [CT]^GGCC[GA]
668 CGGCCG
EagI 505 CGGCCG C^GGCCG
668 CGGCCG
EclXI 505 CGGCCG C^GGCCG
668 CGGCCG
Eco52I 505 CGGCCG C^GGCCG
668 CGGCCG
EcoT14I 1211 CCATGG C^C[AT][AT]GG
2669 CCATGG
KspI 234 CCGCGG CCGC^GG
376 CCGCGG
NaeI 208 GCCGGC GCC^GGC
507 GCCGGC
NcoI 1211 CCATGG C^CATGG
2669 CCATGG
NgoMIV 208 GCCGGC G^CCGGC
507 GCCGGC
NspI 794 GCATGT [GA]CATG^[CT]
1509 ACATGC
PstI 494 CTGCAG CTGCA^G
1795 CTGCAG
SacII 234 CCGCGG CCGC^GG
376 CCGCGG
SapI 1789 GAAGAGC GCTCTTC(1/4)
924 GCTCTTC
StyI 1211 CCATGG C^C[AT][AT]GG
2669 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI