Restriction map for
pFN21AE4311
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AvrII
BamHI BbeI BbrPI BcgI BciVI
BfrI BlnI BsaBI BsaWI BseAI
BsiHKAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FseI HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 71 GGTACC G^GTACC
AccII 166 CGCG CG^CG
AcsI 946 GAATTC [GA]^AATT[CT]
AcyI 771 GACGCC G[GA]^CG[CT]C
ApoI 946 GAATTC [GA]^AATT[CT]
Asp718I 71 GGTACC G^GTACC
AspI 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 265 CCCGAG C^[CT]CG[GA]G
AvaII 756 GGACC G^G[AT]CC
BalI 467 TGGCCA TGG^CCA
BanII 262 GAGCCC G[GA]GC[CT]^C
BbsI 680 GTCTTC GAAGAC(2/6)
BclI 641 TGATCA T^GATCA
BcnI 807 CCCGG CC^[GC]GG
BfaI 76 CTAG C^TAG
BglI 194 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 451 AGATCT A^GATCT
BmyI 262 GAGCCC G[AGT]GC[ACT]^C
BpuAI 680 GTCTTC GAAGAC(2/6)
BsaHI 771 GACGCC G[GA]^CG[CT]C
BsgI 235 CTGCAC GTGCAG(16/14)
BsiWI 908 CGTACG C^GTACG
BsmFI 666 GGGAC GGGAC(10/14)
BsoBI 265 CCCGAG C^[CT]CG[GA]G
Bsp1286I 262 GAGCCC G[AGT]GC[ACT]^C
BspMI 703 GCAGGT ACCTGC(4/8)
BsrBI 403 GAGCGG CCGCTC(-3/-3)
BsrDI 548 GCAATG GCAATG(2/0)
BstUI 166 CGCG CG^CG
BstYI 451 AGATCT [GA]^GATC[CT]
EagI 226 CGGCCG C^GGCCG
EarI 115 CTCTTC CTCTTC(1/4)
EclXI 226 CGGCCG C^GGCCG
Eco52I 226 CGGCCG C^GGCCG
EcoRI 946 GAATTC G^AATTC
FauI 135 GCGGG CCCGC(4/6)
FbaI 641 TGATCA T^GATCA
HaeII 121 AGCGCC [GA]GCGC^[CT]
HgaI 771 GACGC GACGC(5/10)
Hin1I 771 GACGCC G[GA]^CG[CT]C
KpnI 71 GGTACC GGTAC^C
Ksp632I 115 CTCTTC CTCTTC(1/4)
MaeI 76 CTAG C^TAG
MflI 451 AGATCT [GA]^GATC[CT]
MluNI 467 TGGCCA TGG^CCA
MscI 467 TGGCCA TGG^CCA
MvnI 166 CGCG CG^CG
NciI 807 CCCGG CC^[GC]GG
NspI 185 ACATGC [GA]CATG^[CT]
PflFI 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 612 CTGCAG CTGCA^G
SapI 114 GCTCTTC GCTCTTC(1/4)
SexAI 293 ACCAGGT A^CC[AT]GGT
SfcI 612 CTGCAG C^T[GA][CT]AG
SmlI 352 CTCAAG C^T[CT][GA]AG
Tth111I 292 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 813 CCAGATGAAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 451 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 669 ACACGT A^C[GA][CT]GT
867 ACACGT
AlwI 103 GATCC GGATC(4/5)
381 GGATC
AviII 241 TGCGCA TGC^GCA
845 TGCGCA
BanI 71 GGTACC G^G[CT][GA]CC
176 GGTGCC
BlpI 189 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
Bpu1102I 189 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
BsaAI 670 CACGTA [CT]AC^GT[GA]
868 CACGTA
BsaI 297 GGTCTC GGTCTC(1/5)
852 GGTCTC
BseRI 268 GAGGAG GAGGAG(10/8)
441 GAGGAG
BsiEI 222 CGACCG CG[GA][CT]^CG
226 CGGCCG
BsrFI 68 GCCGGT [GA]^CCGG[CT]
224 ACCGGC
BstF5I 282 CATCC GGATG(2/0)
385 CATCC
Cac8I 141 GCTGGC GC[ACGT]^[ACGT]GC
260 GCGAGC
CelII 189 GCTGAGC GC^T[ACGT]AGC
400 GCTGAGC
Cfr10I 68 GCCGGT [GA]^CCGG[CT]
224 ACCGGC
EaeI 226 CGGCCG [CT]^GGCC[GA]
467 TGGCCA
FokI 282 CATCC GGATG(9/13)
385 CATCC
FspI 241 TGCGCA TGC^GCA
845 TGCGCA
MaeII 671 ACGT A^CGT
869 ACGT
MseI 514 TTAA T^TAA
688 TTAA
MslI 670 CACGTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
837 CACTGTGGTG
PflMI 459 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCAGGAAATGG
PleI 564 GACTC GAGTC(4/5)
485 GAGTC
SfaNI 446 GCATC GCATC(5/9)
603 GCATC
TaqI 305 TCGA T^CGA
606 TCGA
TfiI 348 GATTC G^A[AT]TC
506 GATTC
Tru9I 514 TTAA T^TAA
688 TTAA
Tsp509I 627 AATT ^AATT
947 AATT
TthHB8I 305 TCGA T^CGA
606 TCGA
Van91I 459 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
496 CCAGGAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI BbvCI BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstNI CfoI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI TseI Tsp45I
TspRI