Restriction map for
pFN21AE9537
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BpmI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MslI
MunI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 852 GAATTC [GA]^AATT[CT]
AcyI 85 GGCGCC G[GA]^CG[CT]C
AflIII 48 ACACGT A^C[GA][CT]GT
ApaI 499 GGGCCC GGGCC^C
ApoI 852 GAATTC [GA]^AATT[CT]
AspHI 172 GTGCTC G[AT]GC[AT]^C
AvaI 236 CCCGGG C^[CT]CG[GA]G
BalI 573 TGGCCA TGG^CCA
BanI 85 GGCGCC G^G[CT][GA]CC
BbeI 85 GGCGCC GGCGC^C
BbsI 79 GTCTTC GAAGAC(2/6)
BfaI 905 CTAG C^TAG
BlpI 19 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 19 GCTGAGC GC^T[ACGT]AGC
BpuAI 79 GTCTTC GAAGAC(2/6)
BsaHI 85 GGCGCC G[GA]^CG[CT]C
BsiHKAI 172 GTGCTC G[AT]GC[AT]^C
BsmI 567 GAATGC GAATGC(1/-1)
BsoBI 236 CCCGGG C^[CT]CG[GA]G
BsrBI 231 CCGCTC CCGCTC(-3/-3)
BstAPI 10 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 605 AGATCC [GA]^GATC[CT]
CelII 19 GCTGAGC GC^T[ACGT]AGC
DrdI 43 GACAGACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 573 TGGCCA [CT]^GGCC[GA]
EcoRI 852 GAATTC G^AATTC
EcoT14I 167 CCATGG C^C[AT][AT]GG
HaeII 85 GGCGCC [GA]GCGC^[CT]
HgaI 380 GCGTC GACGC(5/10)
Hin1I 85 GGCGCC G[GA]^CG[CT]C
HphI 640 GGTGA GGTGA(8/7)
KasI 85 GGCGCC G^GCGCC
MaeI 905 CTAG C^TAG
MaeII 50 ACGT A^CGT
MflI 605 AGATCC [GA]^GATC[CT]
MluNI 573 TGGCCA TGG^CCA
MscI 573 TGGCCA TGG^CCA
MseI 548 TTAA T^TAA
MspA1I 11 CAGCTG C[AC]G^C[GT]G
NarI 85 GGCGCC GG^CGCC
NcoI 167 CCATGG C^CATGG
PflMI 609 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 11 CAGCTG CAG^CTG
SfaNI 580 GATGC GCATC(5/9)
SfoI 85 GGCGCC GGC^GCC
SmaI 236 CCCGGG CCC^GGG
SmlI 175 CTCAAG C^T[CT][GA]AG
SspI 654 AATATT AAT^ATT
StuI 866 AGGCCT AGG^CCT
StyI 167 CCATGG C^C[AT][AT]GG
Tru9I 548 TTAA T^TAA
Van91I 609 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 605 AGATCC [GA]^GATC[CT]
XmaCI 236 CCCGGG C^CCGGG
XmaI 236 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 222 GATCC GGATC(4/5)
606 GATCC
BanII 460 GAGCCC G[GA]GC[CT]^C
499 GGGCCC
BsgI 257 GTGCAG GTGCAG(16/14)
803 GTGCAG
BsiEI 291 CGACCG CG[GA][CT]^CG
704 CGGTCG
DsaI 167 CCATGG C^C[GA][CT]GG
200 CCGTGG
EarI 552 GAAGAG CTCTTC(1/4)
595 GAAGAG
FauI 230 CCCGC CCCGC(4/6)
184 GCGGG
HapII 237 CCGG C^CGG
465 CCGG
HpaII 237 CCGG C^CGG
465 CCGG
Ksp632I 552 GAAGAG CTCTTC(1/4)
595 GAAGAG
MaeIII 631 GTCAC ^GT[ACGT]AC
641 GTGAC
MspI 237 CCGG C^CGG
465 CCGG
PpuMI 395 GGGTCCC [GA]G^G[AT]CC[CT]
784 AGGACCC
SphI 136 GCATGC GCATG^C
368 GCATGC
TfiI 688 GAATC G^A[AT]TC
826 GAATC
Tsp45I 631 GTCAC ^GT[GC]AC
641 GTGAC
Tsp509I 853 AATT ^AATT
896 AATT
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MboI MboII MnlI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI Sau3AI
Sau96I ScrFI SfcI TseI TspRI