Restriction map for
pFN21AE6526
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HincII HindII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I Van91I XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AciI 175 GCGG CCGC(-3/-1)
AcsI 410 GAATTT [GA]^AATT[CT]
AcyI 183 GACGCC G[GA]^CG[CT]C
AfaI 351 GTAC GT^AC
AlwNI 214 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 132 CCTGAGG CC^T[ACGT]AGG
ApoI 410 GAATTT [GA]^AATT[CT]
AspHI 316 GAGCAC G[AT]GC[AT]^C
AspI 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 432 GGATCC G^GATCC
BanI 20 GGCACC G^G[CT][GA]CC
BbvI 148 GCAGC GCAGC(8/12)
BfaI 73 CTAG C^TAG
BmyI 316 GAGCAC G[AGT]GC[ACT]^C
BpmI 161 CTCCAG CTGGAG(16/14)
BsaHI 183 GACGCC G[GA]^CG[CT]C
BseRI 158 CTCCTC GAGGAG(10/8)
BsiHKAI 316 GAGCAC G[AT]GC[AT]^C
BsiYI 190 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 190 CCCCGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 181 GAGACG CGTCTC(1/5)
BsmFI 442 GGGAC GGGAC(10/14)
Bsp1286I 316 GAGCAC G[AGT]GC[ACT]^C
BspHI 402 TCATGA T^CATGA
BstBI 268 TTCGAA TT^CGAA
BstF5I 312 GGATG GGATG(2/0)
Bsu36I 132 CCTGAGG CC^T[ACGT]AGG
CfoI 151 GCGC GCG^C
EarI 52 GAAGAG CTCTTC(1/4)
Eco81I 132 CCTGAGG CC^T[ACGT]AGG
FokI 312 GGATG GGATG(9/13)
HaeIII 116 GGCC GG^CC
HgaI 183 GACGC GACGC(5/10)
HhaI 151 GCGC GCG^C
Hin1I 183 GACGCC G[GA]^CG[CT]C
HinP1I 151 GCGC G^CGC
HindIII 336 AAGCTT A^AGCTT
HphI 341 TCACC GGTGA(8/7)
Ksp632I 52 GAAGAG CTCTTC(1/4)
MaeI 73 CTAG C^TAG
MaeII 227 ACGT A^CGT
MaeIII 44 GTTAC ^GT[ACGT]AC
MspA1I 173 CAGCGG C[AC]G^C[GT]G
MwoI 97 GCTTTATATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 268 TTCGAA TT^CGAA
PaeR7I 65 CTCGAG C^TCGAG
PflFI 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 169 GACTC GAGTC(4/5)
RcaI 402 TCATGA T^CATGA
RsaI 351 GTAC GT^AC
SfuI 268 TTCGAA TT^CGAA
TfiI 33 GATTC G^A[AT]TC
TseI 148 GCAGC G^C[AT]GC
Tsp509I 411 AATT ^AATT
Tth111I 226 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 72 TCTAGA T^CTAGA
XhoI 65 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 63 AGCT AG^CT
337 AGCT
AvaI 65 CTCGAG C^[CT]CG[GA]G
191 CCCGAG
AvaII 129 GGACC G^G[AT]CC
231 GGTCC
BbsI 76 GAAGAC GAAGAC(2/6)
247 GAAGAC
BpuAI 76 GAAGAC GAAGAC(2/6)
247 GAAGAC
BsaJI 112 CCTGGG C^C[ACGT][ACGT]GG
143 CCAGGG
BsmAI 181 GAGAC GTCTC(1/5)
156 GTCTC
BsoBI 65 CTCGAG C^[CT]CG[GA]G
191 CCCGAG
BstYI 215 AGATCC [GA]^GATC[CT]
432 GGATCC
Cac8I 83 GCAGGC GC[ACGT]^[ACGT]GC
275 GCTGGC
Fnu4HI 148 GCAGC GC^[ACGT]GC
175 GCGGC
HinfI 33 GATTC G^A[ACGT]TC
169 GACTC
ItaI 148 GCAGC GC^[ACGT]GC
175 GCGGC
MflI 215 AGATCC [GA]^GATC[CT]
432 GGATCC
NlaIII 94 CATG CATG^
403 CATG
PstI 81 CTGCAG CTGCA^G
389 CTGCAG
SfcI 81 CTGCAG C^T[GA][CT]AG
389 CTGCAG
SmlI 65 CTCGAG C^T[CT][GA]AG
330 CTCAAG
TaqI 66 TCGA T^CGA
269 TCGA
TthHB8I 66 TCGA T^CGA
269 TCGA
XhoII 215 AGATCC [GA]^GATC[CT]
432 GGATCC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AlwI BsrI BssKI BstNI Cfr13I
DdeI DpnII EcoRII MboI MboII
MnlI MvaI NdeII NlaIV Sau3AI
Sau96I ScrFI TspRI