Restriction map for
pFN21AE6508
##################################
# NO CUT ENZYMES ( 194 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tsp45I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 56 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 132 GGACC G^G[AT]CC
BbvI 67 GCTGC GCAGC(8/12)
BciVI 514 GTATCC GTATCC(6/5)
BfaI 221 CTAG C^TAG
BpmI 266 CTGGAG CTGGAG(16/14)
BsaI 9 GGTCTC GGTCTC(1/5)
BsmAI 10 GTCTC GTCTC(1/5)
BsrI 193 ACTGG ACTGG(1/-1)
BstF5I 424 GGATG GGATG(2/0)
BstYI 233 GGATCT [GA]^GATC[CT]
Cac8I 247 GCTCGC GC[ACGT]^[ACGT]GC
Cfr13I 132 GGACC G^G[ACGT]CC
DdeI 28 CTGAG C^T[ACGT]AG
EcoRV 178 GATATC GAT^ATC
FokI 424 GGATG GGATG(9/13)
HinfI 138 GACTC G^A[ACGT]TC
MaeI 221 CTAG C^TAG
MaeII 37 ACGT A^CGT
MflI 233 GGATCT [GA]^GATC[CT]
MseI 190 TTAA T^TAA
NspI 257 ACATGC [GA]CATG^[CT]
PflFI 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 138 GACTC GAGTC(4/5)
SapI 205 GAAGAGC GCTCTTC(1/4)
Sau96I 132 GGACC G^G[ACGT]CC
ScaI 385 AGTACT AGT^ACT
SexAI 94 ACCAGGT A^CC[AT]GGT
SfcI 62 CTGTAG C^T[GA][CT]AG
SmlI 140 CTCAAG C^T[CT][GA]AG
Tru9I 190 TTAA T^TAA
TseI 67 GCTGC G^C[AT]GC
Tsp509I 173 AATT ^AATT
Tth111I 93 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 233 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 25 GTAC GT^AC
386 GTAC
AluI 66 AGCT AG^CT
430 AGCT
AlwI 355 GATCC GGATC(4/5)
233 GGATC
BsaJI 83 CCAGGG C^C[ACGT][ACGT]GG
308 CCAGGG
BsiYI 77 CCGTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCTTTGATGGG
BslI 77 CCGTTTCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
328 CCTTTGATGGG
DpnII 234 GATC ^GATC
355 GATC
EarI 205 GAAGAG CTCTTC(1/4)
442 GAAGAG
Fnu4HI 4 GCGGC GC^[ACGT]GC
67 GCTGC
ItaI 4 GCGGC GC^[ACGT]GC
67 GCTGC
Ksp632I 205 GAAGAG CTCTTC(1/4)
442 GAAGAG
MboI 234 GATC ^GATC
355 GATC
MwoI 61 GCTGTAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
343 GCATCAACGGC
NdeII 234 GATC ^GATC
355 GATC
NlaIV 87 GGTTCC GG[ACGT]^[ACGT]CC
453 GGTTCC
RsaI 25 GTAC GT^AC
386 GTAC
Sau3AI 234 GATC ^GATC
355 GATC
SfaNI 397 GATGC GCATC(5/9)
343 GCATC
TspRI 20 CAGTG CA[GC]TG[ACGT][ACGT]^
56 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
AciI BssKI BstNI EcoRII MboII
MnlI MvaI NlaIII ScrFI